Functional analysis of cytomegalovirus-specific T lymphocytes compared to tetramer assay in patients undergoing hematopoietic stem cell transplantation

Bone Marrow Transplantation

Volumn 41, Issue 6, 2008, Pages 515-521

  Morita Hoshi, Y ^a,b   Heike, Y ^a   Kawakami, M ^a   Sugita, T ^c   Miura, O ^b   Kim, S W ^a   Mori, S I ^a   Fukuda, T ^a   Tanosaki, R ^a   Tobinai, K ^a   Takaue, Y ^a  

^a NATIONAL CANCER CENTER HOSPITAL   (Japan)

^b TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^c SRL INC   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIVIRUS AGENT; BUSULFAN; CLADRIBINE; CYCLOPHOSPHAMIDE; CYCLOSPORIN; CYTOKINE; FLUDARABINE; GAMMA INTERFERON; GANCICLOVIR; METHOTREXATE; METHYLPREDNISOLONE; TACROLIMUS; TETRAMER; THYMOCYTE ANTIBODY;

ACUTE GRAFT VERSUS HOST DISEASE; ADULT; AGED; ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION; ANTIGEN DETECTION; ARTICLE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CLINICAL ARTICLE; COLITIS; CYTOMEGALOVIRUS INFECTION; CYTOTOXIC T LYMPHOCYTE; DOSE RESPONSE; FUNCTIONAL ASSESSMENT; GRAFT FAILURE; HUMAN; IMMUNOHISTOCHEMISTRY; IMMUNOSURVEILLANCE; MYELOABLATIVE CONDITIONING; PREDICTION; PRIORITY JOURNAL; VIRUS REACTIVATION; WHOLE BODY RADIATION;

ADULT; AGED; ANTIGENS, VIRAL; COLITIS; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; EPITOPES, T-LYMPHOCYTE; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HLA-A ANTIGENS; HUMANS; INTERFERON TYPE II; LYMPHOCYTE COUNT; MIDDLE AGED; PHOSPHOPROTEINS; RISK FACTORS; T-LYMPHOCYTES, CYTOTOXIC; TIME FACTORS; VIRAL MATRIX PROTEINS; VIRUS ACTIVATION;

EID: 41549162925     PISSN: 02683369     EISSN: 14765365     Source Type: Journal    
DOI: 10.1038/sj.bmt.1705932     Document Type: Article

References (28)

1. Boeckh M, Nichols WG, Papanicolaou G, Rubin R, Wingard JR, Zaia J. Cytomegalovirus in hematopoietic stem cell transplant recipients: current status, known challenges, and future strategies. Biol Blood Marrow Transplant 2003; 9: 543-558.
2. Zaia JA, Sissons JG, Riddell S, Diamond DJ, Wills MR, Carmichael AJ et al. Status of Cytomegalovirus Prevention and Treatment in 2000. Hematology (Am Soc Hematol Educ Program) 2000, 339-355.
3. Lacey, SF, Villacres MC, La Rosa C, Wang Z, Longmate J, Martinez J et al. Relative dominance of HLA-B*07 restricted CD8 + T-lymphocyte immune responses to human cytomegalovirus pp65 in persons sharing HLA-A*02 and HLA-B*07 alleles. Hum Immunol 2003; 64: 440-452.
4. Singhal S, Shaw JC, Ainsworth J, Hathway M, Gillespie GM, Paris H et al. Direct visualization and quantitation of cytomegalovirus-specific CD8 + cytotoxic T-lymphocytes in liver transplant patients. Transplantation 2000; 69: 2251-2259.
5. Gratama JW, van Esser JW, Lamers CH, Tournay C, Lowenberg B, Bolhuis RL et al. Tetramer-based quantification of cytomegalovirus (CMV)-specific CD8+ T lyinphocytes in T-cell-depleted stem cell grafts and after transplantation may identify patients at risk for progressive CMV infection. Blood 2001; 98: 1358-1364.
6. Aubert G, Hassan-Walker AF, Madrigal JA, Emery VC, Morte C, Grace S et al. Cytomegalovirus-specific cellular immune responses and viremia in recipients of allogeneic stem cell transplants. J Infect Dis 2001; 184: 955-963.
7. Cwynarski K, Ainsworth J, Cobbold M, Wagner S, Mahendra P, Apperley J et al. Direct visualization of cytomegalovirus-specific T-cell reconstitution after allogeneic stem cell transplantation. Blood 2001; 97: 1232-1240.
8. Engstrand M, Tournay C, Peyrat MA, Eriksson BM, Wadstrom J, Wirgart BZ et al. Characterization of CMVpp65-specific CD8+ T lymphocytes using MHC tetramers in kidney transplant patients and healthy participants. Transplantation 2000; 69: 2243-2250.
9. Lacey SF, Gallez-Hawkins G, Crooks M, Martinez J, Senitzer D, Forman SJ et al. Characterization of cytotoxic function of CMV-pp65-specific CD8 + T-lymphocytes identified by HLA tetramers in recipients and donors of stem-cell transplants. Transplantation 2002; 74: 722-732.
10. Engstrand M, Lidehall AK, Totterman TH, Herrman B, Eriksson BM, Korsgren O. Cellular responses to cytomegalovirus in immunosuppressed patients: circulating CD8 + T cells recognizing CMVpp65 are present but display functional impairment. Clin Exp Immunol 2003; 132: 96-104.
11. Ozdemir E, St John LS, Gillespie G, Rowland-Jones S, Champlin RE, Molldrem JJ et al. Cythmegalovirus reactivation following allogeneic stem cell transplantation is associated with the presence of dysfunctional antigen-specific CD8 + T cells. Blood 2002; 100 3690-3697.
12. Morita Y, Hosokawa M, Ebisawa M, Sugita T, Miura O, Takaue Y et al Evaluation of cytomegalovirus-specific cytotoxic T-lymphocytes in patients with the HLA-A*02 or HLA-A*24 phenotype undergoing homatopoietic stem cell transplantation. Bone Marrow Transplant 2005; 36: 803-811.
13. Morita Y, Heike Y, Kawakami M, Miura O, Nakatsuka S, Ebisawa M et al Monitoring of WT1-specific cytotoxic T lymphocytes after allogeneic hematopoietic stem cell transplantation. Int J Cancer 1006; 119 1360-1367.
14. Rauser G, Einsele H, Sinzger C, Wernet D, Kuntz G, Assenmacher M et al. Rapid generation of combined CMV-specific CD4+ and CD8+ T-cell lines for adoptive transfer into recipients of allogeneic stem cell transplants. Blood 2004; 103: 3565-3572.
15. Tokunaga K, Ishikawa Y, Ogawa A, Wang H, Mitsunaga S, Moriyama S et al. Sequence-based association analysis of HLA class I and II alleles in Japanese supports conservation of common haplotypes. Immunogenetics 1997; 46: 199-205.
16. Waldrop SL, Pitcher CJ, Peterson DM, Maino VC, Picker LJ. Determination of antigen-specific memory/effector CD4+ T cell frequencies by flow cytometry: Evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency. J Clin Invest 1997; 99: 1739-1750.
17. Avetisyan G, Larsson K, Aschan J, Nilsson C, Hassan M, Ljungman P. Impact on the cytomegalovirus (CMV) viral load by CMV-specific T-cell immunity in recipients of allogeneic stem cell transplantation. Bone Marrow Transplant 2006; 38: 687-602.
18. Lifleri D, Gerna G, Fornara C, Lozza L, Maccario R, Locatelli F. Prospective simultaneous quantificatfon of human cytomegalovirus-specific CD4+ and CD8+ T-cell reconstitution in young recipients of allogeneic hematopoietic stem cell transplants. Blood 2006; 108: 1406-1412.
19. Ohnishi M, Sakurai T, Heike Y, Yamazaki R, Kanda Y, Takaue Y et al Evaluation of cytomegalovirus-specific T-cell reconstitution in patients after various allogeneic haematopoietic stem cell transplantation using interferon-gamma-enzyme-linked immunospot and human leucocyte antigen tetramer assays with an immunodominant T-cell epitope. Br J Haematol 2005; 131: 472-479.
20. Karlsson AC, Martin JN, Younger SR, Bredt BM, Epling L, Ronquillo R et al. Comparison of the ELISPOT and cytokine flow cytometry assays for the enumeration of antigen-specific T cells. J Immunol Methods 2003; 283: 141-153.
21. Betts MR, Nason MC, West SM, De Rosa SC, Migueles SA, Abraham J et al HIV nonprogressors preferentially maintain highly functional HIV-specific CD8 + T cells. Blood 2006; 107: 4781-4789.
22. Zimmerli SC, Harari A, Cellerai C, Vallelian F, Bart PA, Pantaleo G. HIV-1-specific IFN-gamma/IL-2-secreting CD8 T cells support CD4-independent proliferation of HIV-1-specific CD8 T cells. Proc Natl Acad Sci USA 2005; 102: 7239-7244.
23. Sinclair E, Tan QX, Sharp M, Girling V, Poon C, Natta MV et al. Protective immunity to cytomegalovirus (CMV) retinitis in AIDS is associated with CMV-specific T cells that express interferon- gamma and interleukin-2 and have a CD8 + cell early maturational phenotype. J Infect Dis 2006; 194: 1537-1546.
24. Foster AE, Gottlieb DJ, Sartor M, Hertzberg MS, Bradstock KF. Cytomegalovirus-specific CD4+ and CD8+ T-cells Follow a similar reconstitution pattern after allogeneic stem cell transplantation. Biol Blood Marrow Transplant 2002; 8: 501-511.
25. Cobbold M, Khan N, Pourgheysari B, Tauro S, McDonald D, Osman H et al Adoptive transfer of cytomegalovirus-specific CTL to stem cell transplant patients after selection by HLA-peptide tetramers. J Exp Med 2005; 202: 379-386.
26. Kern F, Faulhaber N, Frommel C, Khatamzas E, Prosch S, Schonemann C et al. Analysis of CD8 T cell reactivity to cytomegalovirus using protein-spanning pools of overlapping pentadecapeptides. Eur J Immunol 2000; 30: 1676-1682.
27. Bunde T, Kirchner A, Hoffmeister B, Habedank D, Hetzer R, Cherepnev G et al. Protection from cytomegalovirus after transplantation is correlated with immediate early 1-specific CD8 T cells. J Exp Med 2005; 201: 1031-1036.
28. Mohty M, Mohty AM, Blaise D, Faucher C, Bilger K, Isnardon D et al Cytomegalovirus-specific immune recovery following allogeneic HLA-identical sibling transplantation with reduced-intensity preparative regimen. Bone Marrow Transplant 2004; 33: 839-846.