Tetramer-based quantification of cytomegalovirus (CMV)-specific CD8+ T lymphocytes in T-cell-depleted stem cell grafts and after transplantation may identify patients at risk for progressive CMV infection

Blood

Volumn 98, Issue 5, 2001, Pages 1358-1364

  Gratama, Jan W ^a,b   Van Esser, Joost W J ^a,b   Lamers, Cor H J ^a,b   Tournay, Claire ^a,b   Löwenberg, Bob ^a,b   Bolhuis, Reinder L H ^a,b   Cornelissen, Jan J ^a,b  

^a ERASMUS MEDICAL CENTER   (Netherlands)

^b Immunotech   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CD8 ANTIGEN; GANCICLOVIR; HLA A2 ANTIGEN; TETRAMER;

ADOLESCENT; ADULT; ARTICLE; CELLULAR IMMUNITY; CLINICAL ARTICLE; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; CYTOTOXIC T LYMPHOCYTE; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST; FEMALE; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HUMAN; HUMAN CELL; IMMUNE RESPONSE; MALE; MEMORY CELL; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SERODIAGNOSIS; STEM CELL TRANSPLANTATION; T LYMPHOCYTE SUBPOPULATION;

EID: 0035469815     PISSN: 00064971     EISSN: None     Source Type: Journal    
DOI: 10.1182/blood.V98.5.1358     Document Type: Article

References (23)

1. Quinnan GV Jr, Kirmani N, Rook AH, et al. Cytotoxic T cells in cytomegalovirus infection: recovery from cytomegalovirus infection in bone marrow transplant recipients. N Engl J Med. 1982;307:6-13.
2. Reusser P, Riddell SR, Meyers JD, Greenberg PD. Cytotoxic T-lymphocyte response to cytomegalovirus after human allogeneic bone marrow transplantation: pattern of recovery and correlation with cytomegalovirus infection and disease. Blood. 1991;78:1373-1380.
3. Li CR, Greenberg PD, Gilbert MJ, Goodrich JM, Riddell SR. Recovery of HLA-restricted cytomegalovirus (CMV)-specific T-cell responses after allogeneic bone marrow transplant: correlation with CMV disease and effect of ganciclovir prophylaxis. Blood. 1994;83:1971-1979.
4. + virus-specific cytotoxic T lymphocytes. J Med Virol. 1994;43:103-110.
5. Wills MR, Carmichael AJ, Mynard K, et al. The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp65: frequency, specificity and T-cell receptor usage of pp65-specific CTL. J Virol. 1996;70:7569-7579.
6. pos subsets defined by CD57, CD27 and CD45 isoforms. Eur J Immunol. 1999;29:2908-2915.
7. Altman J, Moss PAH, Goulder P, et al. Direct visualization and phenotypic analysis of virus-specific T lymphocytes in HIV-infected individuals. Science. 1996;274:94-96.
8. + T lymphocytes using MHC tetramers in kidney transplanted and healthy participants. Transplantation. 2000;69:2243-2250.
9. Bodinier M, Peyrat MA, Tournay C, et al. Efficient detection and immunomagnetic sorting of specific T cells using multimers of MHC class I and peptide with reduced CD8 binding. Nat Med. 2000;6:707-710.
10. Broers AEC, Van der Holt B, Van Esser JWJ, et al. Increased transplant related morbidity and mortality in CMV seropositive patients despite highly effective prevention of CMV disease after allogeneic T-cell depleted stem cell transplantation. Blood. 2000;95:2240-2245.
11. Middeldorp JM, Jongsma J, Ter Haar A, Schirm J, The TH. Conditions for the detection of IgM and IgG antibodies against cytomegalovirus early and late antigens by ELISA. J Clin Microbiol. 1984;20:763-771.
12. Van der Bij W, Schirm J, Torensma R, Van Son WJ, Tegzess AM, The TH. Comparison between viremia and antigenemia for detection of cytomegalovirus in blood. J Clin Microbiol. 1988;26:2531-2535.
13. Ljungman P, Engelhard D, Link H, et al. Treatment of interstitial pneumonitis due to cytomegalovirus with ganciclovir and intravenous immune globulin: experience of the European Bone Marrow Transplant Group. Clin Infect Dis. 1992;14:831-835.
14. Nicholson JKA, Stein D, Mui T, Mack R, Hubbard M, Denny T. Evaluation of a method for counting absolute numbers of cells with a flow cytometer. Clin Diag Lab Immunol. 1997;4:309-313.
15. Cwynarski K, Ainsworth J, Cobbold M, et al. Direct visualization of cytomegalovirus-specific T-cell reconstitution after allogeneic stem cell transplantation. Blood. 2001;97:1232-1240.
16. Krause H, Hebart H, Jahn G, Müller CA, Einsele H. Screening for CMV-specific T cell proliferation to identify patients at risk of developing late onset CMV disease. Bone Marrow Transplant. 1997;19:1111-1116.
17. Boland GJ, Vlieger AM, Ververs C, De Gast GC. Evidence for transfer of cellular and humoral immunity to cytomegalovirus from donor to recipient in altogeneic bone marrow transplantation. Clin Exp Immunol. 1992;88:506-511.
18. Solache A, Morgan CL, Dodi AI, et al. Identification of three HLA-A*0201-restricted cytotoxic T cell epitopes in the cytomegalovirus protein pp65 that are conserved between eight strains of the virus. J Immunol. 1999;163:5512-5518.
19. + T-cell response to human cytomegalovirus: the 72-kilodalton major immediate early previsited. J Virol. 1999;73:8179-8184.
20. Borysiewicz LK, Hickling JK, Graham S, et al. Human cytomegalovirus-specific cytotoxic T cells: relative frequency of stage-specific CTL recognizing the 72-kD immediate early protein and glycoprotein B expressed by recombinant vaccinia viruses. J Exp Med. 1988;168:919-931.
21. + T cell frequencies by flow cytometry: evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency. J Clin Invest. 1997;99:1739-1750.
22. Kern F, Surel IP, Brock C, et al. T-cell epitope mapping by flow cytometry. Nat Med. 1998;4:975-978.
23. Kern F, Faulhaber N, Frommel C, et al. Analysis of CD8 T cell reactivity to cytomegalovirus using protein-spanning pools of overlapping pentadecapeptides. Eur J Immunol. 2000;30:1676-1682.