Standardization of cytokine flow cytometry assays

BMC Immunology

Volumn 6, Issue , 2005, Pages

  Maecker, Holden T ^a   Rinfret, Aline ^b   D'Souza, Patricia ^c   Darden, Janice ^c   Roig, Eva ^b   Landry, Claire ^b   Hayes, Peter ^d   Birungi, Josephine ^e   Anzala, Omu ^f   Garcia, Miguel ^g   Harari, Alexandre ^g   Frank, Ian ^h   Baydo, Ruth ^h   Baker, Megan ⁱ   Holbrook, Jennifer ⁱ   Ottinger, Janet ⁱ   Lamoreaux, Laurie ^j   Epling, C Lorrie ^k   Sinclair, Elizabeth ^k   Suni, Maria A ^a   Punt, Kara ^l   Calarota, Sandra ^m   El Bahi, Sophia ⁿ   Alter, Gailet ^o   Maila, Hazel ^p   Kuta, Ellen ^q   Cox, Josephine ^q   Gray, Clive ^p   Altfeld, Marcus ^o   Nougarede, Nolwenn ⁿ   Boyer, Jean ^m   Tussey, Lynda ^l   Tobery, Timothy ^l   Bredt, Barry ^k   Roederer, Mario ^j   Koup, Richard ^j   Maino, Vernon C ^a   Weinhold, Kent ⁱ   Pantaleo, Giuseppe ^g   Gilmour, Jill ^d   Horton, Helen ^h   Sekaly, Rafick P ^b   more..

^a BD BIOSCIENCES   (United States)

^b UNIVERSITÉ DE MONTRÉAL   (Canada)

^c NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^d CHELSEA AND WESTMINSTER HOSPITAL   (United Kingdom)

^e UGANDA VIRUS RESEARCH INSTITUTE   (Uganda)

^f UNIVERSITY OF NAIROBI   (Kenya)

^g LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^h UNIVERSITY OF WASHINGTON   (United States)

ⁱ DUKE UNIVERSITY MEDICAL CENTER   (United States)

^j NATIONAL INSTITUTES OF HEALTH   (United States)

^k UNIVERSITY OF CALIFORNIA   (United States)

^l MERCK RESEARCH LABORATORIES   (United States)

^m UNIVERSITY OF PENNSYLVANIA   (United States)

ⁿ SANOFI PASTEUR   (France)

^o MASSACHUSETTS GENERAL HOSPITAL   (United States)

^p NATIONAL INSTITUTE FOR COMMUNICABLE DISEASES   (South Africa)

^q Henry Jackson Foundation   (United States)

more..

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; CD8 ANTIGEN; CYTOKINE; CYTOMEGALOVIRUS ANTIGEN PP65; GAMMA INTERFERON; CYTOMEGALOVIRUS MATRIX PROTEIN 65KDA; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; MATRIX PROTEIN; PHOSPHOPROTEIN;

ACCURACY; ANTIGEN SPECIFICITY; ARTICLE; BLOOD CELL; CELL ACTIVATION; CELL VIABILITY; CELLULAR IMMUNITY; CONSTANTS AND COEFFICIENTS; CONTROLLED STUDY; CRYOPRESERVATION; CYTOKINE FLOW CYTOMETRY; CYTOKINE PRODUCTION; CYTOMEGALOVIRUS INFECTION; FLOW CYTOMETRY; FREEZE DRYING; GOOD LABORATORY PRACTICE; HUMAN; HUMAN CELL; INTRACELLULAR CYTOKINE STAINING; PERIPHERAL BLOOD MONONUCLEAR CELL; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; STANDARDIZATION; T LYMPHOCYTE; BLOOD; BLOOD STORAGE; CHEMISTRY; CLINICAL TRIAL; COMPARATIVE STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EVALUATION; IMMUNOLOGY; LABORATORY; LABORATORY DIAGNOSIS; LYMPHOCYTE; METHODOLOGY; MULTICENTER STUDY; STANDARD;

BLOOD PRESERVATION; CRYOPRESERVATION; CYTOKINES; CYTOMEGALOVIRUS INFECTIONS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; FREEZE DRYING; HUMANS; INDICATORS AND REAGENTS; LABORATORIES; LYMPHOCYTES; PHOSPHOPROTEINS; REPRODUCIBILITY OF RESULTS; SPECIMEN HANDLING; T-LYMPHOCYTES; VIRAL MATRIX PROTEINS;

EID: 26444448037     PISSN: 14712172     EISSN: 14712172     Source Type: Journal    
DOI: 10.1186/1471-2172-6-13     Document Type: Article

References (34)

1. Suni MA, Dunn HS, Orr PL, deLaat R, Sinclair E, Ghanekar SA, Bredt BM, Dunne JF, Maino VC, Maecker HT: Performance of plate-based cytokine flow cytometry with automated data analysis. BMC Immunology 2003, 4:9.
2. Maecker HT: Cytokine flow cytometry. In Flow Cytometry Protocols 2nd edition. Edited by: Hawley TS, Hawley RG. Totowa, NJ, Humana Press; 2004:95-107.
3. Cox JH, Ferrari G, Kalams SA, Lopaczynski W, Oden N, Group ELISPOTS: Results of an ELISPOT proficiency panel conducted in 11 laboratories participating in international human immunodeficiency virus type 1 vaccine trials. AIDS Res Hum Retroviruses 2005 in press.
4. McMichael AJ, Hanke T: HIV vaccines 1983-2003. Nat Med 2003, 9(7):874-880.
5. Pantaleo G, Koup RA: Correlates of immune protection in HIV-1 i nfection: what we know, what we don't know, what we should know. Nat Med 2004, 10(8):806-810.
6. Nomura LE, Walker JM, Maecker HT: Optimization of whole blood antigen-specific cytokine assays for CD4(+) T cells. Cytometry 2000, 40(1):60-68.
7. Landay A: Performance of single cell immune response assays. In NCCLS Standards and Guidelines Wayne, PA, NCCLS; 2004:I/LA26-A.
8. Kern F, Faulhaber N, Frommel C, Khatamzas E, Prosch S, Schonemann C, Kretzschmar I, Volkmer-Engert R, Volk HD, Reinke P: Analysis of CD8 T cell reactivity to cytomegalovirus using protein- spanning pools of overlapping pentadecapeptides. Eur J Immunol 2000, 30(6):1676-1682.
9. Maecker HT, Dunn HS, Suni MA, Khatamzas E, Pitcher CJ, Bunde T, Persaud N, Trigona W, Fu TM, Sinclair E, Bredt BM, McCune JM, Maino VC, Kern F, Picker LJ: Use of overlapping peptide mixtures as antigens for cytokine flow cytometry. J Immunol Methods 2001, 255(1-2):27-40.
10. Kern F, Bunde T, Faulhaber N, Kiecker F, Khatamzas E, Rudawski IM, Pruss A, Gratama JW, Volkmer-Engert R, Ewert R, Reinke P, Volk HD, Picker LJ: Cytomegalovirus (CMV) phosphoprotein 65 makes a large contribution to shaping the T cell repertoire in CMV-exposed individuals. J Infect Dis 2002, 185(12):1709-1716.
11. Kleeberger CA, Lyles RH, Margolick JB, Rinaldo CR, Phair JP, Giorgi JV: Viability and recovery of peripheral blood mononuclear cells cryopreserved for up to 12 years in a multicenter study. Clin Diagn Lab Immunol 1999, 6(1):14-19.
12. Weinberg A, Zhang L, Brown D, Erice A, Polsky B, Hirsch MS, Owens S, Lamb K: Viability and functional activity of cryopreserved mononuclear cells. Clin Diagn Lab Immunol 2000, 7(4):714-716.
13. Currier JR, Kuta EG, Turk E, Earhart LB, Loomis-Price L, Janetzki S, Ferrari G, Birx DL, Cox JH: A panel of MHC class I restricted viral peptides for use as a quality control for vaccine trial ELISPOT assays. J Immunol Methods 2002, 260(1-2):157-172.
14. De Rosa SC, Lu FX, Yu J, Perfetto SP, Falloon J, Moser S, Evans TG, Koup R, Miller CJ, Roederer M: Vaccination in humans generates broad T cell cytokine responses. J Immunol 2004, 173(9):5372-5380.
15. Kuzushima K, Hoshino Y, Fujii K, Yokoyama N, Fujita M, Kiyono T, Kimura H, Morishima T, Morishima Y, Tsurumi T: Rapid determination of Epstein-Barr virus-specific CD8(+) T-cell frequencies by flow cytometry. Blood 1999, 94(9):3094-3100.
16. Moretto WJ, Drohan LA, Nixon DF: Rapid quantification of SIV-specific CD8 T cell responses with recombinant vaccinia virus ELISPOT or cytokine flow cytometry. Aids 2000, 14(16):2625-2627.
17. Asemissen AM, Nagorsen D, Keilholz U, Letsch A, Schmittel A, Thiel E, Scheibenbogen C: Flow cytometric determination of intracellular or secreted IFNgamma for the quantification of antigen reactive T cells. J Immunol Methods 2001, 251(1-2):101-108.
18. Pahar B, Li J, Rourke T, Miller CJ, McChesney MB: Detection of antigen-specific T cell interferon gamma expression by ELISPOT and cytokine flow cytometry assays in rhesus macaques. J Immunol Methods 2003, 282(1-2):103-115.
19. Sun Y, Iglesias E, Samri A, Kamkamidze G, Decoville T, Carcelain G, Autran B: A systematic comparison of methods to measure HIV-1 specific CD8 T cells. J Immunol Methods 2003, 272(1-2) :23-34.
20. Whiteside TL, Zhao Y, Tsukishiro T, Elder EM, Gooding W, Baar J: Enzyme-linked immunospot, cytokine flow cytometry, and tetramers in the detection of T-cell responses to a dendritic cell-based multipeptide vaccine in patients with melanoma. Clin Cancer Res 2003, 9(2):641-649.
21. Karlsson AC, Martin JN, Younger SR, Bredt BM, Epling L, Ronquillo R, Varma A, Deeks SG, McCune JM, Nixon DF, Sinclair E: Comparison of the ELISPOT and cytokine flow cytometry assays for the enumeration of antigen-specific T cells. J Immunol Methods 2003, 283(1-2):141-153.
22. Dunne JF, Maecker HT: Automation of cytokine flow cytometry assays. J Assoc Lab Automation 2004, 9:5-9.
23. Motulsky H: Intuitive Biostatistics. Oxford, Oxford Univ. Press; 1995:199-200.
24. Maecker HT: The role of immune monitoring in evaluating cancer immunotherapy. In Immunotherapy of Cancer Edited by: Disis ML. Totowa, NJ, Humana Press; 2005.
25. Trigona WL, Clair JH, Persaud N, Punt K, Bachinsky M, Sadasivan-Nair U, Dubey S, Tussey L, Fu TM, Shiver J: Intracellular staining for HIV-specific IFN-gamma production: statistical analyses establish reproducibility and criteria for distinguishing positive responses. J Interferon Cytokine Res 2003, 23(7):369-377.
26. Sinclair E, Black D, Epling CL, Carvidi A, Josefowicz SZ, Bredt BM, Jacobson MA: CMV antigen-specific CD4+ and CD8+ T cell IFNgamma expression and proliferation responses in healthy CMV-seropositive individuals. Viral Immunol 2004, 17(3):445-454.
27. Weinberg A, Wohl DA, Brown DG, Pott GB, Zhang L, Ray MG, van der Horst C: Effect of cryopreservation on measurement of cytomegalovirus-specific cellular immune responses in HIV-infected patients. J Acquir Immune Defic Syndr 2000, 25(2):109-114.
28. Costantini A, Mancini S, Giuliodoro S, Butini L, Regnery CM, Silvestri G, Montroni M: Effects of cryopreservation on lymphocyte immunophenotype and function. J Immunol Methods 2003, 278(1-2):145-155.
29. Lathey J: Preliminary steps toward validating a clinical bioassay: case study of the ELISpot assay. Biopharm Intl 2003, March:42-50.
30. Lathey J, Sathiyaseelan J, Matijevic M, Hedley ML: Validation of pretrial ELISspot measurements. BioProcess Intl 2003, Sept.:34-41.
31. Borg L, Kristiansen J, Christensen JM, Jepsen KF, Poulsen LK: Evaluation of accuracy and uncertainty of ELISA assays for the determination of interleukin-4, interleukin-5, interferon-gamma and tumor necrosis factor-alpha. Clin Chem Lab Med 2002, 40(5):509-519.
32. Findlay JW, Smith WC, Lee JW, Nordblom GD, Das I, DeSilva BS, Khan MN, Bowsher RR: Validation of immunoassays for bioanalysis: a pharmaceutical industry perspective. J Pharm Biomed Anal 2000, 21(6):1249-1273.
33. Reimann KA, O'Gorman MR, Spritzler J, Wilkening CL, Sabath DE, Helm K, Campbell DE: Multisite comparison of CD4 and CD8 T-lymphocyte counting by single- versus multiple-platform methodologies: evaluation of Beckman Coulter flow-count fluorospheres and the tetraONE system.The NIAID DAIDS New Technologies Evaluation Group. Clin Diagn Lab Immunol 2000, 7(3):344-351.
34. Koepke JA, Landay AL: Precision and accuracy of absolute lymphocyte counts. Clin Immunol Immunopathol 1989, 52(1):19-27.