Real-time RT-PCR: New approaches for precise mRNA quantification;Real-time RT-PCR: Neue ansätze zur exakten mRNA quantifizierung

BioSpektrum

Volumn 10, Issue 1, 2004, Pages 92-95

  Pfaffl, Michael Walter ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA;

ACCURACY; ANALYTIC METHOD; CORRELATION ANALYSIS; CROSS LINKING; GENOME ANALYSIS; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; MOLECULAR MECHANICS; REACTION ANALYSIS; REACTION OPTIMIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SHORT SURVEY;

EID: 4143060092     PISSN: 09470867     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Short Survey

References (11)

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2. Pfaffl, M.W., and Hageleit, M., (2001): Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR. Biotechnology Letters, 23: 275-282
3. Rasmussen, R. (2001): Quantification on the LightCycler. In: Meuer, S, Wittwer, C, and Nakagawara, K, eds. Rapid Cycle Real-time PCR, Methods and Applications Springer Press, Heidelberg; ISBN 3-540-66736-9, 21-34
4. Pfaffl, M.W. (2001): A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res., 29(9): e45
5. Livak, K.J., and Schmittgen, T.D. (2001): Analysis of relative gene expression data using real-time quantitative PCR and the 2 ∧[-delta delta C(T)] Method. Methods., 25(4): 402-408
6. LightCycler Relative Quantification Software (2001): Version 1.0, Roche Molecular Biochemicals
7. Pfaffl, M.W., Horgan, G.W., and Dempfle, L. (2002): Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res., 30(9): e36
8. Peccoud, J., and Jacob, C. (1996): Theoretical uncertainty of measurements using quantitative polymerase chain reaction. Biophys J., 71(1): 101-108
9. Pfaffl, M.W. (2001): Development and validation of an externally standardised quantitative Insulin like growth factor-1 (IGF-1) RT-PCR using LightCycler SYBR Green I technology. In: Meuer, S., Wittwer, C., and Nakagawara, K., eds.: Rapid Cycle Real-time PCR, Methods and Applications Springer Press, Heidelberg, ISBN 3-540-66736-9, 281-191
10. Tichopad, A., Dzidic, A., and Pfaffl, M.W. (2003): Improving quantitative real-time RT-PCR reproducibility by boosting primer-linked amplification efficiency. Biotechnology Letters 24: 2053-2056
11. Tichopad, A., Dilger, M., Schwarz, G., and Pfaffl, M.W. (2003): Real-time PCR efficiency standardized estimation from single reaction setup. Nucleic Acids Res., 31(20): e122