Detection of virulence factors in Pseudomonas aeruginosa strains isolated from contact lens-associated corneal ulcers

Cornea

Volumn 27, Issue 3, 2008, Pages 320-326

  Pinna, Antonio ^a   Usai, Donatella ^a   Sechi, Leonardo A ^a   Molicotti, Paola ^a   Zanetti, Stefania ^a   Carta, Arturo ^b  

^a UNIVERSITY OF SASSARI   (Italy)

^b UNIVERSITY OF PARMA   (Italy)

Author keywords

Antibiotic susceptibility testing; Bacterial exoproteases; Biofilm formation; Contact lens associated Pseudomonas aeruginosa keratitis; Human corneal epithelial cells; Polymerase chain reaction with enterobacterial repetitive intergenic consensus primers

Indexed keywords

ALKALINE PROTEINASE; AMIKACIN; AMINOGLYCOSIDE ANTIBIOTIC AGENT; AMOXICILLIN PLUS CLAVULANIC ACID; AMPICILLIN; ATROPINE; AZTREONAM; CEFEPIME; CEFOTAXIME; CEFTAZIDIME; CEFTRIAXONE; CIPROFLOXACIN; COTRIMOXAZOLE; ELASTASE; GELATINASE; GENTAMICIN; IMIPENEM; LEVOFLOXACIN; PIPERACILLIN; QUINOLINE DERIVED ANTIINFECTIVE AGENT; SULTAMICILLIN; TOBRAMYCIN; VIRULENCE FACTOR;

ADOLESCENT; ADULT; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATION; BIOFILM; CLINICAL ARTICLE; CONTACT LENS; CORNEA EPITHELIUM; CORNEA ULCER; DNA FINGERPRINTING; EPITHELIUM CELL; FEMALE; HUMAN; KERATITIS; MALE; MICROBIOLOGY; PATHOGENESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

ADOLESCENT; ADULT; BACTERIAL ADHESION; BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; BIOFILMS; COLONY COUNT, MICROBIAL; CONTACT LENSES; CORNEAL ULCER; DRUG RESISTANCE, BACTERIAL; DRUG RESISTANCE, MULTIPLE, BACTERIAL; ENDOPEPTIDASES; EYE INFECTIONS, BACTERIAL; FEMALE; GELATINASES; HUMANS; MALE; MICROBIAL SENSITIVITY TESTS; PANCREATIC ELASTASE; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; VIRULENCE; VIRULENCE FACTORS;

EID: 41149165817     PISSN: 02773740     EISSN: None     Source Type: Journal    
DOI: 10.1097/ICO.0b013e31815c5a3f     Document Type: Article

References (58)

1. Cheng KH, Leung SL, Hoekman HW, et al. Incidence of contact lens-associated microbial keratitis and its related morbidity. Lancet. 1999;354:181-185.
2. Zaidi TS, Fleiszig SM, Preston MJ, et al. Lypopolysaccharide outer core is a ligand for corneal cell binding and ingestion of Pseudomonas aeruginosa. Invest Ophthalmol Vis Sci. 1996;37:976-986.
3. Willcox MD, Holden BA. Contact lens related corneal infections. Biosci Rep. 2001;21:445-461.
4. Pillar CM, Hobden JA. Pseudomonas aeruginosa exotoxin A and keratitis in mice. Invest Ophthalmol Vis Sci. 2002;43:1437-1444.
5. Zegans ME, Becker HI, Budzik J, et al. The role of bacterial biofilms in ocular infections. DNA Cell Biol. 2002;21:415-420.
6. Zolfaghar I, Evans DJ, Fleiszig SMJ. Twitching motility contributes to the role of pili in corneal infection caused by Pseudomonas aeruginosa. Infect Immun. 2003;71:5389-5393.
7. Matsumoto K. Role of bacterial proteases in pseudomonal and serratial keratitis. Biol Chem. 2004;385:1007-1016.
8. Harris A, Torres-Viera C, Venkataraman L, et al. Epidemiology and clinical outcomes of patients with multiresistant Pseudomonas aeruginosa. Clin Infect Dis. 1999;28:1128-1133.
9. Karlowsky JA, Draghi DC, Jones ME, et al. Surveillance for antimicrobial susceptibility among clinical isolates of Pseudomonas aeruginosa and Acinetobacter baumannii from hospitalized patients in the United States, 1998 to 2001. Antimicrob Agents Chemother. 2003;47:1681-1688.
10. Wilhelmus KR, Liesegang TJ, Osato MS, et al. Cumitech 13A: Laboratory Diagnosis of Ocular Infections. Washington, DC: American Society for Microbiology; 1994.
11. Pinna A, Sechi LA, Zanetti S, et al. Detection of virulence factors in a corneal isolate of Klebsiella pneumoniae. Ophthalmology. 2005;112:883-887.
12. Araki-Sasaki K, Ohashi Y, Sasabe T, et al. An SV-40-immortalized human corneal epithelial cell line and its characterization. Invest Ophthalmol Vis Sci. 1995;36:614-621.
13. Fleiszig SM, Zaidi TS, Fletcher EL, et al. Pseudomonas aeruginosa invades corneal epithelial cells during experimental infection. Infect Immun. 1994;62:3485-3493.
14. Sechi LA, Pinna A, Pusceddu C, et al. Molecular characterization and antibiotic susceptibilities of ocular isolates of Staphylococcus epidermidis. J Clin Microbiol. 1999;37:3031-3033.
15. Pinna A, Sechi LA, Zanetti S, et al. Bacillus cereus keratitis associated with contact lens wear. Ophthalmology. 2001;108:1830-1834.
16. Pavlicek A, Hrda S, Flegr J. Free-Tree - freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/jackknife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia. Folia Biol (Praha). 1999;45:97-99.
17. Hampl V, Pavlicek A, Flegr J. Construction and bootstrap analysis of DNA fingerprinting-based phylogenetic trees with the freeware program FreeTree: application to trichomonad parasites. Int J Syst Evol Microbiol. 2001;51:731-735.
18. Page RD. TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci. 1996;12:357-358.
19. Donzis PB, Mondino BJ, Weissman BA, et al. Microbial contamination of contact lens care systems. Am J Ophthalmol. 1987;104:325-333.
20. Gray TB, Curson RTM, Sherwan JF, et al. Acanthamoeba, bacterial, and fungal contamination of contact lens storage cases. Br J Ophthalmol. 1995;79:601-605.
21. Dart JKG, Stapleton F, Minassian D. Contact lenses and other risk factors in microbial keratitis. Lancet. 1991;338:650-653.
22. Weissman BA, Mondino BJ, Pettit TH, et al. Corneal ulcers associated with extended-wear soft contact lenses. Am J Ophthalmol. 1984;97:476-481.
23. Mondino BJ, Weissman BA, Farb MD, et al. Corneal ulcers associated with daily-wear and extended-wear contact lenses. Am J Ophthalmol. 1986;102:58-65.
24. Mayo MS, Cook WL, Schlitzer RL, et al. Antibiograms, serotypes, and plasmid profiles of Pseudomonas aeruginosa associated with corneal ulcers and contact lens wear. J Clin Microbiol. 1986;24:372-376.
25. Alexandrakis G, Alfonso EC, Miller D. Shifting trends in bacterial keratitis in south Florida and emerging resistance to fluoroquinolones. Ophthalmology. 2000;107:1497-1502.
26. Joyanes P, del Carmen Conejo M, Martinez-Martinez L, et al. Evaluation of the VITEK 2 system for the identification and susceptibility testing of three species of nonfermenting gram-negative rods frequently isolated from clinical samples. J Clin Microbiol. 2001;39:3247-3253.
27. Ling TK, Tam PC, Liu ZK, et al. Evaluation of VITEK 2 rapid identification and susceptibility testing system against gram-negative clinical isolates. J Clin Microbiol. 2001;39:2964-2966.
28. Rantakokko-Jalava K, Elo-Lehtonen E, Meurman O. Comparison of workflow and accuracy of identification and antimicrobial susceptibility testing of clinical isolates of Enterobacteriaceae, Pseudomonas aeruginosa and enterococci by Vitek 2 and routine methods. APMIS. 2006;114:43-49.
29. Funke G, Funke-Kissling P. Evaluation of the new Vitek 2 card for identification of clinically relevant Gram-negative rods. J Clin Microbiol. 2004;42:4067-4071.
30. Sader HS, Fritsche TR, Jones RN. Accuracy of three automated systems (MicroScan WalkAway, VITEK, and VITEK 2) for susceptibility testing of Pseudomonas aeruginosa against five broad-spectrum beta-lactam agents. J Clin Microbiol. 2006;44:1101-1104.
31. Juretschko S, Labombardi VJ, Lerner SA, et al. Accuracies of beta-lactam susceptibility test results for Pseudomonas aeruginosa with four automated systems (BD Phoenix, MicroScan WalkAway, Vitek, and Vitek 2). J Clin Microbiol. 2007;45:1339-1342.
32. Obritsch MD, Fish DN, MacLaren R, et al. National surveillance of antimicrobial resistance in Pseudomonas aeruginosa isolates obtained from intensive care unit patients from 1993 to 2002. Antimicrob Agents Chemother. 2004;48:4606-4610.
33. Garg P, Sharma S, Rao GN. Ciprofloxacin-resistant Pseudomonas keratitis. Ophthalmology. 1999;106:1319-1323.
34. Rhee MK, Kowalski RP, Romanowski EG, et al. A laboratory evaluation of antibiotic therapy for ciprofloxacin-resistant Pseudomonas aeruginosa. Am J Ophthalmol. 2004;138:226-230.
35. Costerton JW, Lewandowski Z, DeBeer D, et al. Biofilms, the customized microniche. J Bacteriol. 1994;176:2137-2142.
36. Potera C. Forging a link between biofilms and disease. Science. 1999;283:1837-1839.
37. McLaughlin-Borlace L, Stapleton F, Matheson M, et al. Bacterial biofilm on contact lenses and lens storage cases in wearers with microbial keratitis. J Appl Microbiol. 1998;84:827-838.
38. Stapleton F, Dart J. Pseudomonas keratitis associated with biofilm formation on a disposable soft contact lens. Br J Ophthalmol. 1995;79:864-865.
39. Olofsson AC, Hermansson M, Elwing H. N-acetyl-L-cysteine affects growth, extracellular polysaccharide production and bacterial biofilm formation on solid surfaces. Appl Environ Microbiol. 2003;69:4814-4822.
40. Howe TR, Iglewski BH. Isolation and characterization of alkaline protease-deficient mutant of Pseudomonas aeruginosa in vitro and in a mouse eye model. Infect Immun. 1984;43:1058-1063.
41. Steuhl KP, Doring G, Henni A, et al. Relevance of host-derived and bacterial factors in Pseudomonas aeruginosa corneal infections. Invest Ophthalmol Vis Sci. 1987;28:1559-1568.
42. Kernacki KA, Hobden JA, Hazlett LD. In vivo bacterial proteases production during Pseudomonas aeruginosa corneal infection. Invest Ophthalmol Vis Sci. 1995;36:1371-1378.
43. Doig P, Todd T, Sastry PA, et al. Role of pili in adhesion of Pseudomonas aeruginosa to human respiratory epithelial cells. Infect Immun. 1988;56:1641-1646.
44. Hahn HP. The type-4 pilus is the major virulence-associated adhesion of Pseudomonas aeruginosa - a review. Gene. 1997;192:99-108.
45. Hazlett LD, Moon MM, Singh A, et al. Analysis of adhesion, piliation, protease production and ocular infectivity of several P. aeruginosa strains. Curr Eye Res. 1991;10:351-362.
46. Ramphal R, Sadoff JC, Pyle M, et al. Role of pili in the adherence of Pseudomonas aeruginosa to injured tracheal epithelium. Infect Immun. 1984;44:38-40.
47. Spurr-Michaud SJ, Barza M, Gipson IK. An organ culture system for study of adherence of Pseudomonas aeruginosa to normal and wounded corneas. Invest Ophthalmol Vis Sci. 1988;29:379-386.
48. Gupta SK, Masinick SA, Hobden JA, et al. Bacterial proteases and adherence of Pseudomonas aeruginosa to mouse cornea. Exp Eye Res. 1996;62:641-650.
49. Stern GA, Lubniewski A, Allen C. The interaction between Pseudomonas aeruginosa and the corneal epithelium. Arch Ophthalmol. 1985;103:1221-1225.
50. Fleiszig SM, Zaidi TS, Pier GB. Pseudomonas aeruginosa invasion of and multiplication within corneal epithelial cells in vitro. Infect Immun. 1995;63:4072-4077.
51. Syrmis MW, O'Carroll MR, Sloots TP, et al. Rapid genotyping of Pseudomonas aeruginosa isolates harboured by adult and paediatric patients with cystic fibrosis using repetitive-element-based PCR assays. J Med Microbiol. 2004;53:1089-1096.
52. Fleiszig SMJ, Evans DJ. The pathogenesis of bacterial keratitis: studies with Pseudomonas aeruginosa. Clin Exp Opt. 2002;85:271-278.
53. Fleiszig SMJ. The pathogenesis of contact lens-related keratitis. Optom Vis Sci. 2006;83:866-873.
54. Zhu H, Thuruthyil SJ, Willcox MD. Determination of quorum-sensing signal molecules and virulence factors of Pseudomonas aeruginosa isolates from contact lens-induced microbial keratitis. J Med Microbiol. 2002;51:1063-1070.
55. Sakuragi Y, Kolter R. Quorum-sensing regulation of the biofilm matrix genes (pel) of Pseudomonas aeruginosa. J Bacteriol. 2007;189:5383-5386.
56. Abbas A, Adams C, Scully N, et al. A role for TonB1 in biofilm formation and quorum sensing in Pseudomonas aeruginosa. FEMS Microbiol Lett. 2007;274:269-278.
57. Parsek MR, Val DL, Hanzelka BL, et al. Acyl homoserine-lactone quorum-sensing signal generation. Proc Natl Acad Sci USA. 1999;96:4360-4365.
58. Zhu H, Bandara R, Conibear TC, et al. Pseudomonas aeruginosa with lasI quorum-sensing deficiency during corneal infection. Invest Ophthalmol Vis Sci. 2004;45:1897-1903.