Gel electrophoretic analysis of corneal collagen after photodynamic cross-linking treatment

Cornea

Volumn 27, Issue 3, 2008, Pages 353-356

  Wollensak, Gregor ^a,c   Redl, Bernhard ^b  

^a VIVANTES KLINIKUM NEUKÖLLN   (Germany)

^b INNSBRUCK MEDICAL UNIVERSITY   (Austria)

^c NONE   (Germany)

Author keywords

Collagen; Cornea; Cross linking; Electrophoresis; Riboflavin; UVA

Indexed keywords

COLLAGEN TYPE 1; DODECYL SULFATE SODIUM; POLYACRYLAMIDE GEL; POLYMER; RIBOFLAVIN; SODIUM CHLORIDE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIOCHEMISTRY; CONTROLLED STUDY; CORNEA; CORRELATION ANALYSIS; CROSS LINKING; ELECTROPHORESIS; EPITHELIUM; GEL ELECTROPHORESIS; HEAT; MOLECULAR SIZE; MOLECULAR WEIGHT; NONHUMAN; PHOTODYNAMIC THERAPY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SWINE; ULTRAVIOLET A RADIATION;

ANIMALS; COLLAGEN TYPE I; CORNEA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLAVIN MONONUCLEOTIDE; PHOTOCHEMOTHERAPY; PHOTOSENSITIZING AGENTS; SWINE; ULTRAVIOLET RAYS;

EID: 41149107466     PISSN: 02773740     EISSN: None     Source Type: Journal    
DOI: 10.1097/ICO.0b013e31815cf66a     Document Type: Article

References (39)

1. Wollensak G, Spörl E, Seiler T. Behandlung von Keratokonus durch Kollagenvernetzung. Ophthalmologe. 2003;100:44-49.
2. Wollensak G, Spoerl E, Seiler T. Riboflavin/UV-A-induced collagen crosslinking for the treatment of keratoconus. Am J Ophthalmol. 2003;135:620-627.
3. Wollensak G. Crosslinking treatment of progressive keratoconus: new hope. Curr Opin Ophthalmol. 2006;17:356-360.
4. Wollensak G, Spoerl E, Seiler T. Stress-strain measurements of human and porcine corneas after riboflavin-ultraviolet-A-induced cross-linking. J Cataract Refract Surg. 2003;29:1780-1785.
5. Maffezoli A, Della Pietra A, Rengo S, et al. Photopolymerization of dental composite matrices. Biomaterials. 1994;15:1221-1228.
6. Andreassen TT, Simonsen AH, Oxlund H. Biomechanical properties of keratoconus and normal cornea. Exp Eye Res. 1980;312:435-441.
7. Spoerl E, Wollensak G, Dittert DD, et al. Thermomechanical behavior of collagen-cross-linked porcine cornea. Ophthalmologica. 2004;218:136-140.
8. Wollensak G, Aurich H, Pham D-T, et al. Hydration behavior of porcine cornea crosslinked with riboflavin and ultraviolet A. J Cataract Refract Surg. 2007;33:516-521.
9. Wollensak G, Spoerl E, Wilsch M, et al. Keratocyte apoptosis after corneal collagen-crosslinking using riboflavin/UVA treatment. Cornea. 2004;23:43-49.
10. Wollensak G, Spoerl E, Reber F, et al. Keratocyte cytotoxicity of riboflavin/UVA treatment in vitro. Eye. 2004;18:718-722.
11. Wollensak G, Spoerl E, Reber F, et al. Corneal endothelial cytotoxicity of riboflavin/UVA treatment in vitro. Ophthalmic Res. 2003;35:324-328.
12. Wollensak G, Spoerl E, Wilsch M, et al. Endothelial cell damage after riboflavin-ultraviolet-A treatment in the rabbit. J Cataract Refract Surg. 2003;29:1786-1790.
13. Wollensak G, Seiler T, Wilsch M, et al. Collagen fiber - diameter after riboflavin/UVA induced collagen-crosslinking in the rabbit cornea. Cornea. 2004;23:503-507.
14. Newsome DA, Gross J, Hassell JR. Human corneal stroma contains three distinct collagens. Invest Ophthalmol Vis Sci. 1982;22:376-381.
15. Meisenberg G, Simmons WH. The extracellular matrix. In: Meisenberg G, Simmons WH, eds. Principles of Medical Biochemistry. St. Louis, MO: Mosby; 2006:233-238.
16. von der Mark K. Localization of collagen types in tissues. Int Rev Connect Tissue Res. 1981;9:265-324.
17. Radda TM, Menzel EJ, Freyler H, et al. Collagen types in keratoconus. Graefes Arch Clin Exp Ophthalmol. 1982;218:262-264.
18. Praus R, Brettschneider I, Adam M. Heterogeneitiy of the bovine corneal collagen. Exp Eye Res. 1979;29:469-477.
19. Cintron C, Hong B-S, Kublin CL. Quantitative analysis of collagen from normal developing corneas and corneal scars. Curr Eye Res. 1981;1:1-8.
20. Cintron C, Hong B-S. Heterogeneity of collagens in rabbit cornea: type VI collagen. Invest Ophthalmol Vis Sci. 1988;29:760-766.
21. Laemmli WK. Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature. 1970;227:680-685.
22. Bailey AJ, Sims TJ. Chemistry of the collagen cross-links. Biochem J. 1976;153:211-215.
23. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-254.
24. Cintron C, Hong B-S, Covington HI, et al. Heterogeneitiy of collagens in rabbit cornea: type III collagen. Invest Ophthalmol Vis Sci. 1988;29:767-775.
25. Newsome DA, Foidart J-M, Hassell JR, et al. Detection of specific collagen types in normal and keratoconus corneas. Invest Ophthalmol Vis Sci. 1981;20:738-750.
26. Mencucci R, Mazzotta C, Rossi F, et al. Riboflavin and ultraviolet A collagen crosslinking: in vivo thermographic analysis of the corneal surface. J Cataract Refract Surg. 2007;33:1005-1008.
27. Gerrard JA, Brown PK, Fayle SE. Maillard crosslinking of food proteins I: the reaction of glutaraldehyde, formaldehyde and glyceraldehyde with ribonuclease. Food Chem. 2002;79:343-349.
28. H- crystallin in riboflavin-sensitized photooxidation. Exp Eye Res. 1988;47:1-15.
29. Goosey JD, Zigler JS, Kinoshita JH. Cross-linking of lens crystallins in a photodynamic system: a process mediated by singlet oxygen. Science. 1980;208:1278-1280.
30. Zigler JS, Goosey JD. Singlet oxygen as a possible factor in human senile nuclear cataract development. Curr Eye Res. 1984;3:59-65.
31. Lee K-W, Simpson G, Ortwerth B. A systematic approach to evaluate the modification of lens proteins by glycation-induced crosslinking. Biochim Biophys Acta. 1999;1453:141-151.
32. Akiba J, Ueno N, Chakrabarti B. Mechanisms of photo-induced vitreous liquefaction. Curr Eye Res. 1994;13:505-512.
33. Hikichi T, Akiba J, Ueno N, et al. Cross-linking of vitreous collagen and degradation of hyaluronic acid induced by bilirubin-sensitized photochemical reaction. Jpn J Ophthalmol. 1997;41:154-159.
34. Akiba J, Ueno N, Chakrabarti B. Age-related changes in the molecular properties of vitreous collagen. Curr Eye Res. 1993;12:951-954.
35. Spoerl E, Wollensak G, Seiler T. Increased resistance of crosslinked cornea against enzymatic digestion. Curr Eye Res. 2003;29:35-40.
36. Wollensak G, Iomdina E. Long term biomechanical properties of rabbit cornea after photodynamic collagen-crosslinking. Acta Ophthalmol. (in press).
37. Kato Y, Uchida K, Kawakishi S. Aggregation of collagen exposed to UVA in the presence of riboflavin: a plausible role of tyrosine modification. Photochem Photobiol. 1994;59:343-349.
38. Foote CS. Definition of type I and type II photosensitized oxidation. Photochem Photobiol. 1991;54:659.
39. Bailey AJ, Paul RG, Knott L. Mechanisms of maturation and ageing of collagen. Mech Ageing Dev. 1998;106:1-56.