Antibody index and specific antibody quotient in horses after intragastric administration of Sarcocystis neurona sporocysts

American Journal of Veterinary Research

Volumn 69, Issue 3, 2008, Pages 403-409

  Heskett, Katherine A ^a,b   MacKay, Robert J ^a  

^a University of Florida   (United States)

^b NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIPROTOZOAL AGENT; IMMUNOGLOBULIN G; PONAZURIL; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANTIBODY DETECTION; ANTIBODY SPECIFICITY; ARTICLE; BLOOD SAMPLING; CEREBROSPINAL FLUID; CONTROLLED STUDY; DRUG INTERMITTENT THERAPY; ENZYME LINKED IMMUNOSORBENT ASSAY; HORSE DISEASE; IMMUNODIFFUSION; IMMUNOGLOBULIN BLOOD LEVEL; INOCULATION; NONHUMAN; PROTOZOAL INFECTION; SARCOCYSTIS NEURONA; SPOROCYST;

ANIMALS; ANTIBODIES, PROTOZOAN; ANTIBODY SPECIFICITY; ENCEPHALOMYELITIS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HORSE DISEASES; HORSES; IMMUNODIFFUSION; IMMUNOGLOBULIN G; SARCOCYSTIS; SARCOCYSTOSIS;

EQUIDAE; SARCOCYSTIS NEURONA;

EID: 41049115220     PISSN: 00029645     EISSN: None     Source Type: Journal    
DOI: 10.2460/ajvr.69.3.403     Document Type: Article

References (41)

1. Dubey JP, Lindsay DS, Saville WJ, et al. A review of Sarcocystis neurona and equine protozoal myeloencephalitis (EPM). Vet Parasitol 2001;95:89-131.
2. Dubey JP, Davis SW, Speer CA, et al. Sarcocystis neurona n. sp. (Protozoa: Apicomplexa), the etiologic agent of equine protozoal myeloencephalitis. J Parasitol 1991;77:212-218.
3. Fenger CK, Granstrom DE, Langemeier JL, et al. Identification of opossums (Didelphis virginiana) as the putative definitive host of Sarcocystis neurona. J Parasitol 1995;81:916-919.
4. Granstrom DE, Dubey JP, Davis SW et al. Equine protozoal myeloencephalitis: antigen analysis of cultured Sarcocystis neurona merozoites. J Vet Diagn Invest 1993;5:88-90.
5. Dubey JP, Mitchell SM, Morrow JK, et al. Prevalence of antibodies to Neospora caninum, Sarcocystis neurona, and Toxoplasma gondii in mild horses from central Wyoming. J Parasitol 2003;89:716-720.
6. Duarte PC, Daft BM, Conrad PA, et al. Evaluation and comparison of an indirect fluorescent antibody test for detection of antibodies to Sarcocystis neurona, using serum and cerebrospinal fluid of naturally and experimentally infected, and vaccinated horses. J Parasitol 2004;90:379-386.
7. Granstrom DE. Recent advances in the laboratory diagnosis of equine parasitic diseases. Vet Clin North Am Equine Pract 1995; 11:437-442.
8. Duarte PC, Daft BM, Conrad PA, et al. Comparison of a serum indirect fluorescent antibody test with two Western blot tests for the diagnosis of equine protozoal myeloencephalitis. J Vet Diagn Invest 2003;15:8-13.
9. Granstrom DE. Diagnosis of equine protozoal myeloencephalitis: western blot analysis, in Proceedings. 11th Annu Meet Am Coll Vet Intern Med Forum 1993;587-589.
10. Daft BM, Barr BC, Gardner IA, et al. Sensitivity and specificity of western blot testing of cerebrospinal fluid and serum for diagnosis of equine protozoal myeloencephalitis in horses with and without neurologic abnormalities. J Am Vet Med Assoc 2002;221:1007-1013.
11. Bernard WV. Equine protozoal myelitis - laboratory tests and interpretation, in Proceedings. Int Equine Neurol Conf 1997;7-11.
12. Miller MM, Sweeney CR, Russell GE, et al. Effects of blood contamination of cerebrospinal fluid on western blot analysis for detection of antibodies against Sarcocystis neurona and on albumin quotient and immunoglobulin G index in horses. J Am Vet Med Assoc 1999;215:67-71.
13. Witonsky S, Morrow JK, Leger C, et al. Sarcocystis neurona-specific immunoglobulin G in the serum and cerebrospinal fluid of horses administered S neurona vaccine. J Vet Intern Med 2004;18:98-103.
14. Reiber H. Dynamics of brain-derived proteins in cerebrospinal fluid. Clin Chim Acta 2001;310:173-186.
15. Reiber H, Peter JB. Cerebrospinal fluid analysis: disease-related data patterns and evaluation programs. J Neurol Sci 2001;184:101-122.
16. Felgenhauer K, Reiber H. The diagnostic significance of antibody specificity indices in multiple sclerosis and herpes virus induced diseases of the nervous system. Clin Invest 1992;70:28-37.
17. Reiber H, Lange P. Quantification of virus-specific antibodies in cerebrospinal fluid and serum: sensitive and specific detection of antibody synthesis in brain. Clin Chem 1991;37:1153-1160.
18. Goldmann H, Witmer R. Antikorper im Kammerwasser. Ophthalmologica 1954;127:323-330.
19. Ganrot K, Laurell CB. Measurement of IgG and albumin content of cerebrospinal fluid, and its interpretation. Clin Chem 1974;20:571-573.
20. Andrews FM, Geiser DR, Sommardahl CS, et al. Albumin quotient, IgG concentration, and IgG index determinations in cerebrospinal fluid of neonatal foals. Am J Vet Res 1994;55:741-745.
21. Tumani H, Nolker G, Reiber H. Relevance of cerebrospinal fluid variables for early diagnosis of neuroborreliosis. Neurology 1995;45:1663-1670.
22. Dorta-Contreras AJ, Reiber H. Intrathecal synthesis of immunoglobulins in eosinophilic meningoencephalitis due to Angiostrongylus cantonensis. Clin Diagn Lab Immunol 1998;5:452-455.
23. Contini C, Fainardi E, Cultrera R, et al. Advanced laboratory techniques for diagnosing Toxoplasma gondii encephalitis in AIDS patients: significance of intrathecal. production and comparison with PCR and ECL-western blotting. J Neuroimmunol 1998;92:29-37.
24. Lejon V, Reiber H, Legros D, et al. Intrathecal immune response pattern for improved diagnosis of central nervous system involvement in trypanosomiasis. J Infect Dis 2003;187:1475-1483.
25. Sofaly CD, Reed SM, Gordon JC, et al. Experimental induction of equine protozoan myeloencephalitis (EPM) in the horse: effect of Sarcocystis neurona sporocyst inoculation dose on the development of clinical neurologic disease. J Parasitol 2002;88:1164-1170.
26. Cutler TJ, MacKay RJ, Ginn PE, et al. Immunoconversion against Sarcocystis neurona in normal and dexamethasone-treated horses challenged with S. neurona sporocysts. Vet Parasitol 2001;95:197-210.
27. Fenger CK, Granstrom DE, Gajadhar AA, et al. Experimental induction of equine protozoal myeloencephalitis in horses using Sarcocystis sp. sporocysts from the opossum (Didelphis virginiana). Vet Parasitol 1997;68:199-213.
28. Saville WJ, Sofaly CD, Reed SM, et al. An equine protozoal myeloencephalitis challenge model testing a second transport after inoculation with Sarcocystis neurona sporocysts. J Parasitol 2004;90:1406-1410.
29. Saville WJ, Stich RW, Reed SM, et al. Utilization of stress in the development of an equine model for equine protozoal myeloencephalitis. Vet Parasitol 2001;95:211-222.
30. MacKay RJ, Tanhauser ST, Gillis KD, et al. Effect of intermittent oral administration of ponazuril on experimental Sarcocystis neurona infection of horses. Am J Vet Res 2008;69:403-409.
31. Cutler TJ, MacKay RJ, Ginn PE, et al. Are Sarcocystis neurona and Sarcocystis falcatula synonymous? A horse infection challenge. J Parasitol 1999;85:301-305.
32. Andrews FM, Maddux JM, Faulk D. Total protein, albumin quotient, IgG and IgG index determinations for horse cerebrospinal fluid. Prog Vet Neurol 1996;1:197-204.
33. Long MT, Hines MT, Knowles DR et al. Sarcocystis neurona: parasitemia in a severe combined immunodeficient (SCID) horse fed sporocysts. Exp Parasitol 2002;100:150-154.
34. Harlow E, Lane DP. Immunoassays. In: Harlow E, Lane DR eds. Antibodies: a laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 1988;553-613.
35. Shapiro SS, Wilk MB. An analysis of variance test for normality (complete samples). Biometrika 1965;52:591-611.
36. Levene H. Robust tests for equality of variance. In: Olkin I, Ghurye SG, Hoeffding W et al, eds. Contributions to probability and statistics: Essays in honor of Harold Hotelling. Palo Alto, Calif: Stanford University Press, 1960;278-292.
37. Hoane JS, Morrow JK, Saville WJ, et al. Enzyme-linked immunosorbent assays for detection of equine antibodies specific to Sarcocystis neurona surface antigens. Clin Diagn Lab Immunol 2005;12:1050-1056.
38. Marsh AE, Hyun C, Barr BC, et al. Characterization of monoclonal antibodies developed against Sarcocystis neurona. Parasitol Res 2002;88:501-506.
39. Wilske B. Microbiological diagnosis in Lyme borreliosis. Int J Med Microbiol 2002;291(suppl 33):114-119.
40. Reiber H. Flow rate of cerebrospinal fluid (CSF) - a concept common to normal blood-CSF barrier function and to dysfunction in neurological diseases. J Neurol Sci 1994;122:189-203.
41. Murphy JE, Marsh AE, Reed SM, et al. Development and evaluation of a Sarcocystis neurona-specific IgM capture enzyme-linked immunosorbent assay. J Vet Intern Med 2006;20:322-328.