A primer-introduced restriction analysis-polymerase chain reaction method to detect knockdown resistance mutations in Anopheles gambiae

Journal of Medical Entomology

Volumn 45, Issue 2, 2008, Pages 237-241

  Janeira, F ^a   Vicente, J L ^a   Kanganje, Y ^a   Moreno, M ^b,c   Do Rosario V E ^a   Cravo, P ^a   Pinto, J ^a  

^a INSTITUTO DE HIGIENE E MEDICINA TROPICAL   (Portugal)

^b Red de Investigación de Centros de Enfermedades Tropicales RICET Madrid   (Spain)

^c NONE   (Spain)

Author keywords

Anopheles gambiae; Kdr mutations; PIRA PCR; Resistance

Indexed keywords

INSECTICIDE; PRIMER DNA; PYRETHROID; SODIUM CHANNEL;

AMINO ACID SUBSTITUTION; ANIMAL; ANOPHELES GAMBIAE; ARTICLE; FEMALE; GENETICS; INSECTICIDE RESISTANCE; METHODOLOGY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

AMINO ACID SUBSTITUTION; ANIMALS; ANOPHELES GAMBIAE; DNA MUTATIONAL ANALYSIS; DNA PRIMERS; FEMALE; INSECTICIDE RESISTANCE; INSECTICIDES; POLYMERASE CHAIN REACTION; PYRETHRINS; SODIUM CHANNELS;

ANOPHELES GAMBIAE;

EID: 41049098666     PISSN: 00222585     EISSN: None     Source Type: Journal    
DOI: 10.1603/0022-2585(2008)45[237:APRACR]2.0.CO;2     Document Type: Article

References (15)

1. Bass, C., D. Nikou, M. J. Donnelly, M. S. Williamson, H. Ranson, A. Ball, J. Vontas, and L. M. Field. 2007. Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods. Malar. J. 6: 111.
2. Collins, F. H., M. A. Mendez, M. O. Rasmussen, P. C. Mehaffey, N. J. Besansky, and V. Finnerty. 1987. A ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex. Am. J. Trop. Med. Hyg. 37: 37-41.
3. della Torre, A., C. Fanello, M. Akogbeto, J. Dossou-yovo, G. Favia, V. Petrarca, and M. Coluzzi. 2001. Molecular evidence of incipient speciation within Anopheles gambiae s.s. in West Africa. Insect Mol. Biol. 10: 9-18.
4. Etang, J., E. Fondjo, F. Chandre, I. Morlais, C. Brengues, P. Nwane, M. Chouaibou, H. Ndjemai, and F. Simard. 2006. First report of knockdown mutations in the malaria vector Anopheles gambiae from Cameroon. Am. J. Trop. Med. Hyg. 74: 795-797.
5. Fanello, C., F. Santolamazza, and A. della Torre. 2002. Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP. Med. Vet. Entomol. 16: 461-464.
6. Ke, X., A. Collins, and S. Ye. 2001. PIRA PCR designer for restriction analysis of single nucleotide polymorphisms. Bioinformatics 17: 838-839.
7. Kulkarni, M., M. Rowland, M. Alifrangis, F. W. Mosha, J. Matowo, R. Malima, J. Peter, E. Kweka, I. Lyimo, S. Magesa, A. Salanti, M. E. Rau, and C. Drakeley. 2006. Occurrence of the leucine-to-phenylalanine knockdown resistance (kdr) mutation in Anopheles arabiensis populations in Tanzania, detected by a simplified high-throughput SSOP-ELISA method. Malar. J. 5: 56.
8. Lynd, A., H. Ranson, P. J. McCall, N. P. Randle, W. C. Black 4th, E. D. Walker, and M. J. Donnelly. 2005. A simplified high-throughput method for pyrethroid knock-down resistance (kdr) detection in Anopheles gambiae. Malar J. 4: 16.
9. Martinez-Torres, D., F. Chandre, M. S. Williamson, F. Darriet, J. B. Bergé, A. L. Devonshire, P. Guillet, N. Pasteur, and D. Pauron. 1998. Molecular characterization of pyrethroid knockdown resistance (kdr) in the major malaria vector Anopheles gambiae s.s. Insect Mol. Biol. 7: 179-184.
10. Moreno, M., J. L. Vicente, J. Cano, P. J. Berzosa, A. de Lucio, S. Nzambo, L. Bobuakasi, J. N. Buatiche, M. Ondo, F. Micha, V. E. do Rosario, J. Pinto, and A. Benito. 2008. Knockdown resistance mutations (kdr) and insecticide susceptibility to DDT and pyrethroids in Anopheles gambiae from Equatorial Guinea. Trop. Med. Int. Health (in press).
11. Pinto, J., A. Lynd, N. Elissa, M. J. Donnelly, C. Costa, G. Gentile, A. Caccone, and V. E. do Rosário. 2006. Co-occurrence of East and West African kdr mutations suggests high levels of resistance to pyrethroid insecticides in Anopheles gambiae from Libreville, Gabon. Med. Vet. Entomol. 20: 27-32.
12. Ranson, H., B. Jensen, J. M. Vulule, X. Wang, J. Hemingway, and F. H. Collins. 2000. Identification of a point mutation in the voltage-gated sodium channel gene of Kenyan Anopheles gambiae associated with resistance to DDT and pyrethroids. Insect Mol. Biol. 9: 491-497.
13. Tripet, F., J. Wright, and G. Lanzaro. 2006. A new high-performance PCR diagnostic for the detection of pyrethroid knockdown resistance kdr in Anopheles gambiae. Am. J. Trop. Med. Hyg. 74: 658-662.
14. Verhaeghen, K., W. Van Bortel, P. Roelants, T. Backeljau, and M. Coosemans. 2006. Detection of the East and West African kdr mutation in Anopheles gambiae and Anopheles arabiensis from Uganda using a new assay based on FRET/Melt Curve analysis. Malar. J. 5: 16.
15. [WHO] World Health Organization. 2006. WHO gives indoor use of DDT a clean bill of health for controlling malaria, (www.who.int/mediacentre/news/ releases/2006/pr50/en/print.html).