Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP

Medical and Veterinary Entomology

Volumn 16, Issue 4, 2002, Pages 461-464

  Fanello, C ^a   Santolamazza, F ^b   Della Torre, Ale ^b  

^a LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Africa; Anopheles gambiae complex; Malaria vectors; Molecular markers; Mosquito identification; PCR RFLP; Sibling species

Indexed keywords

DISEASE VECTOR;

AFRICA; AGING; ANIMALS; ANOPHELES; BASE SEQUENCE; GENES, INSECT; LARVA; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SPECIES SPECIFICITY;

ANOPHELES GAMBIAE; CULICIDAE; DIPTERA; INSECTA;

EID: 0036917844     PISSN: 0269283X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2915.2002.00393.x     Document Type: Article

References (13)

1. Coetzee, M., Craig, M. & Le Sueur, D. (2000) Distribution of African malaria mosquitoes belonging to the Anopheles gambiae complex. Parasitology Today, 16, 74-77.
2. Coluzzi, M., Sabatini, A., Petrarca, V. & Di Deco, M.A. (1979) Chromosomal differentiation and adaptation to human environment in the Anopheles gambiae complex. Transaction of the Royal Society of Tropical Medicine and Hygiene, 73, 483-497.
3. Coluzzi, M., Petrarca, V. & Di Deco, M.A. (1985) Chromosomal inversion intergradation and incipient speciation in Anopheles gambiae. Bollettino di Zoologia, 52, 45-63.
4. Coluzzi, M., Sabatini, A., della Torre, A., Di Deco, M.A. & Petrarca, V. (2002) A polytene chromosome analysis of the Anopheles gambiae species complex. Science, in press.
5. della Torre, A., Costantini, C., Besansky, N.J., Caccone, A., Petrarca, V., Powell, J.R. & Coluzzi, M. (2002) Speciation within Anopheles gambiae: The glass is half full. Science, 298, 115-116.
6. della Torre, A., Fanello, C., Akogbeto, M., Dossou-yovo, J., Favia, G., Petrarca, V. & Coluzzi, M. (2001) Molecular evidence of incipient speciation within Anopheles gambiae s.s. in West Africa. Insect Molecular Biology, 10, 9-18.
7. Favia, G., della Torre, A., Bagayoko, M., Lanfrancotti, A., Sagnon, N., Touré, Y.T. & Coluzzi, M. (1997) Molecular identification of sympatric chromosomal forms of Anopheles gambiae and further evidence of their reproductive isolation. Insect Molecular Biology, 6, 377-383.
8. Favia, G., Lanfrancotti, A., Spanos, L., Siden-Kiamos, I. & Louis, C. (2001) Molecular characterization of ribosomal DNA polymorphisms discriminating among chromosomal forms of Anopheles gambiae s.s. Insect Molecular Biology, 10, 19-23.
9. Fettene, M., Koekemoer, L.L., Hunt, R.H. & Coetzee, M. (2002) PCR assay for identification of Anopheles quadriannulatus species B from Ethiopia and other sibling species of the Anopheles gambiae complex. Medical and Veterinary Entomology, 16, 214-217.
10. Gentile, G., Slotman, M., Ketmaier, V., Powell, J.R. & Caccone, A. (2001) Attempts to molecularly distinguish cryptic taxa in Anopheles gambiae s.s. Insect Molecular Biology, 10, 25-32.
11. Scott, J.A., Brogdon, W.G. & Collins, F.H. (1993) Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction. American Journal of Tropical Medicine and Hygiene, 49, 520-529.
12. Townson, H. & Onapa, A.W. (1994) Identification by rDNA-PCR of Anopheles bwambae, a geothermal spring species of the An. gambiae complex. Insect Molecular Biology, 3, 279-282.
13. Wondji, C., Simard, F. & Fontenille, D. (2002) Evidence for genetic differentiation between the molecular forms M and S within the Forest chromosomal form of Anopheles gambiae in an area of sympatry. Insect Molecular Biology, 11, 11-19.