Genetic typing of the 56-kDa type-specific antigen gene of contemporary Orientia tsutsugamushi isolates causing human scrub typhus at two sites in north-eastern and western Thailand

FEMS Immunology and Medical Microbiology

Volumn 52, Issue 3, 2008, Pages 335-342

  Blacksell, Stuart D ^a,b   Luksameetanasan, Rungnapa ^a   Kalambaheti, Thareerat ^a   Aukkanit, Nuntipa ^a   Paris, Daniel H ^a,b   McGready, Rose ^a,b,c   Nosten, François ^a,b,c   Peacock, Sharon J ^a,b   Day, Nicholas P J ^a,b  

^a MAHIDOL OXFORD TROPICAL MEDICINE RESEARCH UNIT   (Thailand)

^b JOHN RADCLIFFE HOSPITAL   (United Kingdom)

^c SHOKLO MALARIA RESEARCH UNIT   (Thailand)

Author keywords

56 kDa; Orientia tsutsugamushi; Phylogenetic; Scrub typhus; Senotype; Thailand

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATION; CONTROLLED STUDY; GENE SEQUENCE; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; ORIENTIA TSUTSUGAMUSHI; PHYLOGENY; PRIORITY JOURNAL; SCRUB TYPHUS; THAILAND;

ANTIGENS, BACTERIAL; DNA, BACTERIAL; GENOTYPE; HUMANS; ORIENTIA TSUTSUGAMUSHI; PHYLOGENY; SCRUB TYPHUS; SEQUENCE ANALYSIS, DNA; THAILAND;

EID: 41049091520     PISSN: 09288244     EISSN: 1574695X     Source Type: Journal    
DOI: 10.1111/j.1574-695X.2007.00375.x     Document Type: Article

References (26)

1. Audy JR Harrison JL (1951) A review of investigations on mite typhus in Burma and Malaya, 1945-1950. Trans R Soc Trop Med Hyg 44 : 371 404.
2. Coleman RE, Sangkasuwan V, Suwanabun N et al 2002) Comparative evaluation of selected diagnostic assays for the detection of IgG and IgM antibody to Orientia tsutsugamushi in Thailand. Am J Trop Med Hyg 67 : 497 503.
3. Elisberg BL, Campbell JM Bozeman FM (1968) Antigenic diversity of Rickettsia tsutsugamushi: epidemiologic and ecologic significance. J Hyg Epidemiol Microbiol Immunol 12 : 18 25.
4. Enatsu T, Urakami H Tamura A (1999) Phylogenetic analysis of Orientia tsutsugamushi strains based on the sequence homologies of 56-kDa type-specific antigen genes. FEMS Microbiol Lett 180 : 163 169.
5. Hanson B (1985) Identification and partial characterization of Rickettsia tsutsugamushi major protein immunogens. Infect Immun 50 : 603 609.
6. Khuntirat B, Lerdthusnee K, Leepitakrat W, Kengluecha A, Wongkalasin K, Monkanna T, Mungviriya S, Jones JW Coleman RE (2003) Characterization of Orientia tsutsugamushi isolated from wild-caught rodents and chiggers in northern Thailand. Ann NY Acad Sci 990 : 205 212.
7. Kim IS, Seong SY, Woo SG, Choi MS Chang WH (1993) High-level expression of a 56-kilodalton protein gene (bor56) of Rickettsia tsutsugamushi Boryong and its application to enzyme-linked immunosorbent assays. J Clin Microbiol 31 : 598 605.
8. Kollars TM, Bodhidatta D, Phulsuksombati D, Tippayachai B Coleman RE (2003) Short report: variation in the 56-kD type-specific antigen gene of Orientia tsutsugamushi isolated from patients in Thailand. Am J Trop Med Hyg 68 : 299 300.
9. Kumar S, Tamura K Nei M (2004) MEGA3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5 : 150 163.
10. Leelarasamee A, Chupaprawan C, Chenchittikul M Udompanthurat S (2004) Etiologies of acute undifferentiated febrile illness in Thailand. J Med Assoc Thai 87 : 464 472.
11. Luksameetanasan R, Blacksell SD, Kalambaheti T et al 2007) Patient and sample-related factors that effect the success of in vitro isolation of Orientia tsutsugamushi. Southeast Asian J Trop Med Public Health 38 : 91 96.
12. Manosroi J, Chutipongvivate S, Auwanit W Manosroi A (2006) Determination and geographic distribution of Orientia tsutsugamushi serotypes in Thailand by nested polymerase chain reaction. Diagn Microbiol Infect Dis 55 : 185 190.
13. Ohashi N, Tamura A, Ohta M Hayashi K (1989) Purification and partial characterization of a type-specific antigen of Rickettsia tsutsugamushi. Infect Immun 57 : 1427 1431.
14. Ohashi N, Nashimoto H, Ikeda H Tamura A (1992) Diversity of immunodominant 56-kDa type-specific antigen (TSA) of Rickettsia tsutsugamushi. Sequence and comparative analyses of the genes encoding TSA homologues from four antigenic variants. J Biol Chem 267 : 12728 12735.
15. Phongmany S, Rolain JM, Phetsouvanh R et al 2006) Rickettsial infections and fever, Vientiane, Laos. Emerg Infect Dis 12 : 256 262.
16. Qiang Y, Tamura A, Urakami H, Makisaka Y, Koyama S, Fukuhara M Kadosaka T (2003) Phylogenetic characterization of Orientia tsutsugamushi isolated in Taiwan according to the sequence homologies of 56-kDa type-specific antigen genes. Microbiol Immunol 47 : 577 583.
17. Seong SY, Huh MS, Jang WJ, Park SG, Kim JG, Woo SG, Choi MS, Kim IS Chang WH (1997) Induction of homologous immune response to Rickettsia tsutsugamushi Boryong with a partial 56-kilodalton recombinant antigen fused with the maltose-binding protein MBP-Bor56. Infect Immun 65 : 1541 1545.
18. Seong SY, Kim MK, Lee SM, Odgerel Z, Choi MS, Han TH, Kim IS, Kang JS Lim BU (2000) Neutralization epitopes on the antigenic domain II of the Orientia tsutsugamushi 56-kDa protein revealed by monoclonal antibodies. Vaccine 19 : 2 9.
19. Shirai A, Tanskul PL, Andre RG, Dohany AL Huxsoll DL (1981) Rickettsia tsutsugamushi strains found in chiggers collected in Thailand. Southeast Asian J Trop Med Public Health 12 : 1 6.
20. Stover CK, Marana DP, Carter JM, Roe BA, Mardis E Oaks EV (1990) The 56-kilodalton major protein antigen of Rickettsia tsutsugamushi: molecular cloning and sequence analysis of the sta56 gene and precise identification of a strain-specific epitope. Infect Immun 58 : 2076 2084.
21. Suttinont C, Losuwanaluk K, Niwatayakul K et al 2006) Causes of acute, undifferentiated, febrile illness in rural Thailand: results of a prospective observational study. Ann Trop Med Parasitol 100 : 363 370.
22. Tamura A, Ohashi N, Urakami H, Takahashi K Oyanagi M (1985) Analysis of polypeptide composition and antigenic components of Rickettsia tsutsugamushi by polyacrylamide gel electrophoresis and immunoblotting. Infect Immun 48 : 671 675.
23. Tamura A, Yamamoto N, Koyama S, Makisaka Y, Takahashi M, Urabe K, Takaoka M, Nakazawa K, Urakami H Fukuhara M (2001) Epidemiological survey of Orientia tsutsugamushi distribution in field rodents in Saitama Prefecture, Japan, and discovery of a new type. Microbiol Immunol 45 : 439 446.
24. Tay ST, Rohani YM, Ho TM Shamala D (2005) Sequence analysis of the hypervariable regions of the 56 kDa immunodominant protein genes of Orientia tsutsugamushi strains in Malaysia. Microbiol Immunol 49 : 67 71.
25. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 : 4876 4882.
26. Watt G Parola P (2003) Scrub typhus and tropical rickettsioses. Curr Opin Infect Dis 16 : 429 436.