In vitro diffusion barriers of the mouse jejunum in Ussing chambers

Journal of Nutritional Science and Vitaminology

Volumn 54, Issue 1, 2008, Pages 30-38

  Inagaki Tachibana, Eiko ^a   Natori, Yasuo ^a   Hayashi, Hisayoshi ^b   Suzuki, Yuichi ^b  

^a NAGOYA UNIVERSITY OF ARTS AND SCIENCES   (Japan)

^b UNIVERSITY OF SHIZUOKA   (Japan)

Author keywords

Chloride secretion; Gastrointestinal injury; Intestinal barrier; Mucosal defense

Indexed keywords

CALCIUM; ELECTROLYTE; FORSKOLIN; SODIUM CHLORIDE;

ANIMAL TISSUE; ARTICLE; DIFFUSION CHAMBER; ELECTRIC CONDUCTIVITY; EPITHELIUM; JEJUNUM; MALE; MOUSE; NONHUMAN; POTENTIAL DIFFERENCE;

ANIMALS; BIOLOGICAL TRANSPORT, ACTIVE; CELL MEMBRANE PERMEABILITY; DIFFUSION; DIFFUSION CHAMBERS, CULTURE; ELECTRIC CONDUCTIVITY; EPITHELIAL CELLS; FLUORESCENT DYES; FORSKOLIN; INTESTINAL MUCOSA; ISOQUINOLINES; JEJUNUM; MALE; MEMBRANE POTENTIALS; MICE; PERMEABILITY; TIME FACTORS;

EID: 40949159395     PISSN: 03014800     EISSN: 18817742     Source Type: Journal    
DOI: 10.3177/jnsv.54.30     Document Type: Article

References (21)

1. Ma TY, Anderson JM. 2006. Tight junctions and the intestinal barrier. In: Physiology of the Gastrointestinal Tract (Johnson LR, Barrett KE, Ghishan FK, eds), Vol 2, p 1559-1594. Academic Press, London.
2. MacDonald TT, Monteleone G. 2005. Immunity, inflammation, and allergy in the gut. Science 307 (5717): 1920-1925.
3. Allen A, Flemström G. 2005. Gastroduodenal mucus bicarbonate barrier: protection against acid and pepsin. Am J Physiol 288: C1-C19.
4. Dietrich CG, Geier A, Oude Elferink RP. 2003. ABC of oral bioavailability: transporters as gatekeepers in the gut. Gut 52: 1788-1795.
5. Montrose MH, Akiba Y, Takeuchi K, Kaunitz JD. 2006. Gastroduodenal mucosal defense. In: Physiology of the Gastrointestinal Tract (Johnson LR, Barrett KE, Ghishan FK, eds), Vol 2, p 1259-1291. Academic Press, London.
6. Powell DW. 1981. Barrier function of epithelia. Am J Physiol 241: G275-G288.
7. Watson AJM, Chu S, Sieck L, Gerasimenko O, Bullen T, Campbell F, McKenna M, Rose T, Montrose MH. 2005. Epithelial barrier function in vivo is sustained despite gaps in epithelial layers. Gastroenterology 129: 902-912.
8. Inagaki E, Natori Y, Ohgishi Y, Hayashi H, Suzuki Y. 2005. Segmental difference of mucosal damage along the length of a mouse small intestine in Ussing chambers. J Nutr Sci Vitaminol 51: 406-412.
9. Takeichi M. 1988. The Cadherins: cell-cell adhesion molecules controlling animal morphogenesis. Development 102: 639-655.
10. Benjamin MA, McKay DM, Yang PC, Cameron H, Perdue MH. 2000. Glucagon-like peptide-2 enhances intestinal epithelial barrier function of both transcellular and paracellular pathways in the mouse. Gut 47: 112-119.
11. Cameron HL, Perdue MH. 2005. Stress impairs murine intestinal barrier function: improvement by glucagon-like peptide-2. J Pharmacol Exp Ther 314: 214-220.
12. Fromm M, Schulzke JD, Hegel U. 1985. Epithelial and subepithelial contributions to transmural electrical resistance of intact rat jejunum in vitro. Pflügers Arch 405: 400-402.
13. Polentarutti BI, Peterson AL, Sjöberg SK, Anderberg EK, Utter LM, Ungell AL. 1999. Evaluation of viability of excised rat intestinal segments in the Ussing chamber: investigation of morphology, electrical parameters, and permeability characteristics. Pharm Res 16 (3): 446-454.
14. Söderholm JD, Hedman L, Artursson P, Franzén L, Larsson J, Pantzar N, Permert J, Olaison G. 1998. Integrity and metabolism of human ileal mucosa in vitro in the Ussing chamber. Acta Physiol Scand 162: 47-56.
15. Bajka BH, Gillespie CM, Steeb CB, Read LC, Howarth GS. 2003. Applicability of the Ussing chamber technique to permeability determinations in functionally distinct regions of the gastrointestinal tract in the rat. Scand J Gastroenterol 38: 732-741.
16. Esposito G, Faelli A, Tosco M, Burlini N, Capraro V. 1979. Extracellular space determination in rat small intestine by using markers of different molecular weights. Pflügers Arch 382: 67-71.
17. Naftalin RJ, Tripathi S. 1985. Passive water flows driven across the isolated rabbit ileum by osmotic, hydrostatic and electrical gradients. J Physiol 360: 27-50.
18. Bode L, Eklund EA, Murch S, Freeze HH. 2005. Heparan sulfate depletion amplifies TNF-α-induced protein leakage in an in vitro model of protein-losing enteropathy. Am J Physiol 288: G1015-G1023.
19. Powell DW, Adegboyega PA, DiMari JF, Mifflin RC. 2005. Epithelial cells and their neighbors I. Role of intestinal myofibroblasts in development, repair, and cancer. Am J Physiol Gastrointest Liver Physiol 289: G2-G7.
20. Jezyk N, Rubas W, Grass GM. 1992. Permeability characteristics of various intestinal regions of rabbit, dog, and monkey. Pharm Res 9 (12): 1580-1586.
21. Nejdfors P, Ekelund M, Jeppsson B, Westrom BR. 2000. Mucosal in vitro permeability in the intestinal tract of the pig, the rat, and man: species- and region-related differences. Scand J Gastroenterol 35: 501-507.