Statistical assessment of the accuracy and precision of bacteria- and virus-sized microsphere enumerations by epifluorescence microscopy

Water Research

Volumn 42, Issue 6-7, 2008, Pages 1431-1440

  Chae, Gi Tak ^a   Stimson, Jesse ^b   Emelko, Monica B ^b   Blowes, David W ^b   Ptacek, Carol J ^b   Mesquita, Maria M ^b  

^a Korea Institute of Geosceince and Mineral Resources   (South Korea)

^b UNIVERSITY OF WATERLOO   (Canada)

Author keywords

Enumeration; Epifluorescence microscopy; Pathogen sized microsphere; Precision and accuracy; Statistical assessment

Indexed keywords

CALCITE; CONCENTRATION (PROCESS); GROUNDWATER FLOW; IMPURITIES; IONIC STRENGTH; PARTICLE SIZE; PATHOGENS; POTABLE WATER;

EPIFLUORESCENCE MICROSCOPY; PATHOGEN-SIZED MICROSPHERE; STATISTICAL ASSESSMENT;

BACTERIA;

GROUND WATER; MICROSPHERE;

BACTERIA; CALCITE; CONCENTRATION (PROCESS); GROUNDWATER FLOW; IMPURITIES; IONIC STRENGTH; PARTICLE SIZE; PATHOGENS; POTABLE WATER;

BACTERIUM; COLLOID; CONCENTRATION (COMPOSITION); DRINKING WATER; POLLUTANT TRANSPORT; STATISTICAL ANALYSIS; VIRUS;

ACCURACY; ARTICLE; BACTERIAL COUNT; BACTERIUM DETECTION; EPIFLUORESCENCE MICROSCOPY; IONIC STRENGTH; NONHUMAN; PRIORITY JOURNAL; STATISTICAL ANALYSIS; VIRUS DETECTION; VIRUS TITRATION; WATER ANALYSIS; WATER CONTAMINATION;

BACTERIA (MICROORGANISMS);

EID: 40849124331     PISSN: 00431354     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.watres.2007.10.015     Document Type: Article

References (29)

1. Alonso M.C., Rodriques J., and Borrego J.J. Enumeration and isolation of viral particles from oligotrophic marine environments by tangential flow filtration. Int. Microbiol. 2 (1999) 227-232
2. APHA (American Public Health Association), AWWA (American Water Works Association), and WEF (Water Environmental Federation), 2005. In: Eaton, A.D., Clesceri, L.S., Rice, E.W., Greenberg, A.E., Franson, M. A. H. (Eds.), Standard Methods for the Examination of Water and Wastewater. Washington, DC, 1368pp.
3. Bales R.C., Li S., Yeh T.-C.J., Lenczewski M., and Gerba C.P. Bacteriophage and microspheres transport in saturated porous media: forced-gradient experiment at Borden, Ontario. Water Resour. Res. 33 4 (1997) 639-648
4. Becker M.W., Reimus P.W., and Vilks P. Transport and attenuation of carboxylate-modified microspheres in fractured rock laboratory and field tracer tests. Ground Water 37 3 (1999) 387-395
5. Becker M.W., Metge D.W., Collins S.A., Shapiro A.M., and Harvey R.W. Bacterial transport experiments in fractured crystalline bedrock. Ground Water 41 5 (2003) 682-689
6. Close M.E., Pang L., Flintoft M.J., and Sinton L.W. Distance and flow effects on microsphere transport in a large gravel column. J. Environ. Qual. 35 4 (2006) 1204-1212
7. Danovaro R., Dell'Anno A., Trucco A., Serresi M., and Vanucci S. Determination of virus abundance in marine sediments. Appl. Environ. Microbiol. 67 3 (2001) 1384-1387
8. Delie F. Evaluation of nano- and microparticle uptake by the gastrointestinal tract. Adv. Drug Delivery Rev. 34 (1998) 221-233
9. Gitis V., Adin A., Nasser A., Gun J., and Lev O. Fluorescent dye labeled bacteriophages-a new tracer for the investigation of viral transport in porous media: 1. Introduction and characterization. Water Res. 36 17 (2002) 4227-4234
10. Harvey R.W., Kinner N.E., MacDonald D., Metge D.W., and Bunn A. Role of physical heterogeneity in the interpretation of small-scale laboratory and field observations of bacteria, microbial-sized microsphere, and bromide transport through aquifer sediments. Water Resour. Res. 29 8 (1993) 2713-2722
11. Harvey R.W., Kinner N.E., Bunn A., MacDonald D., and Metge D. Transport behavior of groundwater protozoa and protozoan-sized microspheres in sandy aquifer sediments. Appl. Environ. Microbiol. 61 1 (1995) 209-217
12. Hobbie J.E., Daley R.J., and Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 33 5 (1977) 1225-1228
13. Jones J.G., and Simon B.M. An investigation of errors in direct counts of aquatic bacteria by epifluorescence microscopy, with reference to a new method for dyeing membrane filters. J. Appl. Bacteriol. 39 (1975) 317-329
14. Kirchman D., Sigda J., Kapuscinski R., and Mitchell R. Statistical analysis of the direct count method for enumerating bacteria. Appl. Environ. Microbiol. 44 2 (1982) 376-382
15. Lisle J.T., Hamilton M.A., Willse A.R., and McFeters G.A. Comparison of fluorescence microscopy and solid-phase cytometry methods for counting bacteria in water. Appl. Environ. Microbiol. 70 9 (2004) 5343-5348
16. McCarthy J.F., and Zachara J.M. Subsurface transport of contaminants: mobile colloids in the subsurface environment may alter the transport of contaminants. Environ. Sci. Technol. 23 5 (1989) 496-502
17. McCarthy J.F., McKay L.D., and Bruner D.D. Influence of ionic strength and cation charge on transport of colloidal particles in fractured shale saprolite. Environ. Sci. Technol. 36 17 (2002) 3735-3743
18. Montanié H., Hartmann H.J., Crottereau C., and Trichet C. Virus-like particle analysis in a seston-rich coastal pond using transmission electron microscopy. Aquat. Microb. Ecol. 28 2 (2002) 105-115
19. Moulin V., and Ouzounian G. Role of colloids and humic substances in the transport of radio-elements through the geosphere. Appl. Geochem. Suppl. 1 (1992) 179-186
20. Niehren S., and Kinzelbach W. An all-solid-state flow cytometer for counting fluorescent microspheres. Anal. Chem. 67 15 (1995) 2666-2671
21. Ryan J.N., Elimelech M., Ard R.A., Harvey R.W., and Johnson P.R. Bacteriophage PRD1 and silica colloid transport and recovery in an iron oxide-coated sand aquifer. Environ. Sci. Technol. 33 1 (1999) 63-73
22. Saiers J.E., and Lenhart J.J. Ionic-strength effects on colloid transport and interfacial reactions in partially saturated porous media. Water Resour. Res. 39 (2003) 1256
23. Schijven J.F., and Hassanizadeh S.M. Removal of viruses by soil passage: overview of modeling, processes, and parameters. Crit. Rev. Environ. Sci. Technol. 30 1 (2000) 49-127
24. Shapiro H.M. Practical Flow Cytometry (1995), Wiley-Liss, New York 542pp
25. Suttle C.A. Enumeration and isolation of viruses. In: Kemp P.F. (Ed). Handbook of Methods in Aquatic Microbial Ecology (1993), Lewis Publishers, Boca Raton, FL 121-134
26. Wen K., Ortmann A.C., and Suttle C.A. Accurate estimation of viral abundance by epifluorescence microscopy. Appl. Environ. Microbiol. 70 7 (2004) 3862-3867
27. Winer B.J. Statistical Principles in Experimental Design (1971), McGraw-Hill, New York 907pp
28. Zhang P., Johnson W.P., Piana M.J., Fuller C.C., and Naftz D.L. Potential artifacts in interpretation of differential breakthrough of colloids and dissolved tracers in the context of transport in a zero-valent iron permeable reactive barrier. Ground Water 39 6 (2001) 831-840
29. Zhuang J., Qi J., and Jin Y. Retention and transport of amphiphilic colloids under unsaturated flow conditions: effect of particle size and surface property. Environ. Sci. Technol. 39 20 (2005) 7853-7859