Enumeration and isolation of viral particles from oligotrophic marine environments by tangential flow filtration

International Microbiology

Volumn 2, Issue 4, 1999, Pages 227-232

  Carmen Alonso, M ^a   Rodrîguez, Jaimc ^a   Borrego, Juan J ^a  

^a UNIVERSITY OF MÁLAGA   (Spain)

Author keywords

Oligotrophic environmentsmarine viruses; Tangential flow filtration; Transmission electron microscopy; Virus enumeration

Indexed keywords

SEA WATER;

ADSORPTION; ARTICLE; BACTERIUM; CENTRIFUGATION; ELECTRON MICROSCOPY; FILTRATION; ISOLATION AND PURIFICATION; MEDITERRANEAN SEA; METHODOLOGY; MICROBIOLOGY; PARTICLE SIZE; ULTRAFILTRATION; VIROLOGY; VIRUS;

ADSORPTION; BACTERIA; CENTRIFUGATION; FILTRATION; MEDITERRANEAN SEA; MICROSCOPY, ELECTRON; PARTICLE SIZE; SEAWATER; ULTRAFILTRATION; VIROLOGY; VIRUSES; WATER MICROBIOLOGY;

EID: 0033288609     PISSN: 11396709     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Ackermann HW ( 1992) Frequency of morphological phage description. ArchVirol 124:201-209
2. Barthel KG, Schneider G, Gradinger R, Lenz J (1989) Concentration of pico- and nanoplankton by means of tangential flow filtration. J Plankton Res 11:1213-1221
3. Bergh O, Borsheim KY, Bratbak G, Heldal M (1989) High abundance of viruses found in aquatic environments. Nature 340:467-468
4. Herman D, Rohr ME, Safferman RS (1980) Concentration of poliovirus in water by molecular filtration. Appl Environ Microbiol 40:426-428
5. Borsheim KY, Bratbak G, Heldal M (1990) Enumeration and biomass estimation of planktonic bacteria and viruses by transmission electron microscopy. Appl Environ Microbiol 56:352-356
6. Bradley DE (1967) Ultrastructure of bacteriophages and bacteriocins. Bacteriol Rev 31:230-314
7. Fuhrman JA, Suttle CA (1993) Viruses in marine planktonic systems. Oceanography 6:51-63
8. Giovannoni SJ, DeLong EF, Schmidt TM, Pace NR (1990) Tangential flow filtration and preliminary phylogenetic analysis of marine picoplankton. Appl Environ Microbiol 56:2572-2575
9. Guixa-Boixareu N, Calderön-Paz JI, Heldal M, Bratbak G, Pedrôs-Aliô C (1996) Viral lysis and bacterivory as prokaryotic loss factors along a salinity gradient. Aquat Microb Ecol 11:215-227
10. Hara S, Terauchi K, Koike I (1991) Abundance of viruses in marine waters: assessment by epifluorescence and transmission electron microscopy. Appl Environ Microbiol 57:2731-2734
11. Havelaar AH, Hogeboom WH (1983) Factors affecting the enumeration of coliphages in sewage and sewage-polluted water. Antonie van Leeuwenhoek J Microbiol 49:387-397
12. Hennés KP, Suttle CA (1995) Direct counts of viruses in natural waters and laboratory cultures by epifluorescence microscopy. Limnol Oceanogr 40:1050-1055
13. Katzenelson E, Fallal B, Hostovesky T (1976) Organic flocculation: an efficient second-step concentration of viruses in tap water. Appl Environ Microbiol 32:638-639
14. Paul JH, Jiang SC, Rose JB (1991) Concentration of viruses and dissolved DNA from aquatic environments by vortex flow filtration. Appl Environ Microbiol 57:2197-2204
15. Porter KG, Feig YS ( 1980) The use of DAP1 for identifying and counting aquatic microflora. Limnol Oceanogr 25:943-948
16. Proctor LM, Fuhrman JA ( 1990) Viral mortal ity of marine bacteria and cyanobacteria. Nature 343:60-62
17. Rodrfguez V, Bautista B, Jimenez-Gomez F, Guerrero F, Blanco JM, Rodrfguez J (1998) Loss of pico- and nanoplankton cells during concentration by tangential flow filtration. J Plankton Res 20:1087-1097
18. Shibley GP, Manousos M, Munch M, Zelljadt I, Fisher L, Mayyasi S, Harewood K, Stevens R, Jensen KE (1980) New method for large-scale growth and concentration of the Epstein-Barr viruses. Appl Environ Microbiol 40:1044-1048
19. Suttle CA, Chan AM, Cottrell MT (1990) Infection of phytoplankton by viruses and reduction of primary productivity. Nature 347:467-469
20. Suttle CA, Chan AM, Cottrell MT (1991) Use of ultrafiltration to isolate viruses from seawater which are pathogens of marine phytoplankton. Appl Environ Microbiol 57:721-726
21. Torrella F, Morita RY (1979) Evidence for a high incidence of bacteriophage particles in the waters of Yaquina Bay, Oregon: ecological and taxonomical implications. AppI Environ Microbiol 37:774-778
22. Watanabe RA, Fryer JL, Rohovec JS (1988) Molecular filtration for recovery of waterborne viruses of fish. Appl Environ Microbiol 54:1606-1609
23. Weinbauer MG, Fuks D, Peduzzi P (1993) Distribution of viruses and dissolved DNA along a coastal trophic gradient in the northern Adriatic Sea. Appl Environ Microbiol 59:4047-4084
24. Wommack KE, Hill RT, Kessel M, Russek-Cohen E, Colwell RR (1992) Distribution of viruses in the Chesapeake Bay. Appl Environ Microbiol 58:2965-2970