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Let's get specific: the relationship between specificity and affinity
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Shows that the factors that contribute to high affinity are the same as those that determine specificity.
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Eaton B.E., Gold L., and Zichi D.A. Let's get specific: the relationship between specificity and affinity. Chem Biol 2 (1995) 633-638. Shows that the factors that contribute to high affinity are the same as those that determine specificity.
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Schneider D.J., Feigon J., Hostomsky Z., and Gold L. High-affinity ssDNA inhibitors of the reverse transcriptase of type 1 human immunodeficiency virus. Biochemistry 34 (1995) 9599-9610
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Wiley-VCH, Weinheim. An important collection of articles about aptamers that spans the range from practical to theoretical.
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Klussmann S. The Aptamer Handbook: Functional Oligonucleotides and their Applications (2006), Wiley-VCH, Weinheim. An important collection of articles about aptamers that spans the range from practical to theoretical.
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(2006)
The Aptamer Handbook: Functional Oligonucleotides and their Applications
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Klussmann, S.1
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7
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High-throughput proteomics using antibody microarrays: an update
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A useful review of the state of the art of antibody microarrays.
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Borrebaeck C.A., and Wingren C. High-throughput proteomics using antibody microarrays: an update. Expert Rev Mol Diagn 7 (2007) 673-686. A useful review of the state of the art of antibody microarrays.
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Expert Rev Mol Diagn
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Borrebaeck, C.A.1
Wingren, C.2
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Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project
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This work foreshadows the complete elucidation of the human transcriptome, and leads to the possibility that far more of the genome than expected appears as RNA - one wonders if all such RNAs are important or represent noise from unavoidably 'sloppy' DNA-dependent RNA polymerases.
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Birney E., Stamatoyannopoulos J.A., Dutta A., Guigo R., Gingeras T.R., Margulies E.H., Weng Z., Snyder M., Dermitzakis E.T., Thurman R.E., et al. Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. Nature 447 (2007) 799-816. This work foreshadows the complete elucidation of the human transcriptome, and leads to the possibility that far more of the genome than expected appears as RNA - one wonders if all such RNAs are important or represent noise from unavoidably 'sloppy' DNA-dependent RNA polymerases.
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(2007)
Nature
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Birney, E.1
Stamatoyannopoulos, J.A.2
Dutta, A.3
Guigo, R.4
Gingeras, T.R.5
Margulies, E.H.6
Weng, Z.7
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Dermitzakis, E.T.9
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A short overview of the HUPO Antibody Initiative and the Human Protein Atlas program (http://www.proteinatlas.org/).
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Uhlen M. Mapping the human proteome using antibodies. Mol Cell Proteomics 6 (2007) 1455-1456. A short overview of the HUPO Antibody Initiative and the Human Protein Atlas program (http://www.proteinatlas.org/).
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Uhlen, M.1
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13
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Identification of differentially expressed proteins in ovarian cancer using high-density protein microarrays
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Uses protein microarrays to detect autoantibodies in sera to identify proteins associated with ovarian cancer.
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Hudson M.E., Pozdnyakova I., Haines K., Mor G., and Snyder M. Identification of differentially expressed proteins in ovarian cancer using high-density protein microarrays. Proc Natl Acad Sci U S A 104 (2007) 17494-17499. Uses protein microarrays to detect autoantibodies in sera to identify proteins associated with ovarian cancer.
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Proc Natl Acad Sci U S A
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Hudson, M.E.1
Pozdnyakova, I.2
Haines, K.3
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Design of recombinant antibody microarrays for complex proteome analysis: choice of sample labeling-tag and solid support
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Wingren C., Ingvarsson J., Dexlin L., Szul D., and Borrebaeck C.A. Design of recombinant antibody microarrays for complex proteome analysis: choice of sample labeling-tag and solid support. Proteomics 7 (2007) 3055-3065
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A description of the state of the art in recombinant antibody microarrays.
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Wingren C., Steinhauer C., Ingvarsson J., Persson E., Larsson K., and Borrebaeck C.A. Microarrays based on affinity-tagged single-chain Fv antibodies: sensitive detection of analyte in complex proteomes. Proteomics 5 (2005) 1281-1291. A description of the state of the art in recombinant antibody microarrays.
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Wingren, C.1
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Identification of protein expression signatures associated with Helicobacter pylori infection and gastric adenocarcinoma using recombinant antibody microarrays
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Ellmark P., Ingvarsson J., Carlsson A., Lundin B.S., Wingren C., and Borrebaeck C.A. Identification of protein expression signatures associated with Helicobacter pylori infection and gastric adenocarcinoma using recombinant antibody microarrays. Mol Cell Proteomics 5 (2006) 1638-1646
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26
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Serum protein markers for early detection of ovarian cancer
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Mor G., Visintin I., Lai Y., Zhao H., Schwartz P., Rutherford T., Yue L., Bray-Ward P., and Ward D.C. Serum protein markers for early detection of ovarian cancer. Proc Natl Acad Sci U S A 102 (2005) 7677-7682
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Ward, D.C.9
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27
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33645241277
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Array-based proteomics: mapping of protein circuitries for diagnostics, prognostics, and therapy guidance in cancer
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A description of the drawbacks and advantages of several formats of microarrays used in proteomics.
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Sheehan, K.M.2
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Dimensionality is the issue: use of photoaptamers in protein microarrays
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Yet another discussion of obstacles on the path to high-density proteomics.
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Petach H., and Gold L. Dimensionality is the issue: use of photoaptamers in protein microarrays. Curr Opin Biotechnol 13 (2002) 309-314. Yet another discussion of obstacles on the path to high-density proteomics.
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Petach, H.1
Gold, L.2
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0000359208
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Kinetic proofreading: a new mechanism for reducing errors in biosynthetic processes requiring high specificity
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One of our favorite papers of all time - a clear exposition about the limitations of equilibrium binding when searching for a needle in a haystack.
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Hopfield J.J. Kinetic proofreading: a new mechanism for reducing errors in biosynthetic processes requiring high specificity. Proc Natl Acad Sci U S A 71 (1974) 4135-4139. One of our favorite papers of all time - a clear exposition about the limitations of equilibrium binding when searching for a needle in a haystack.
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(1974)
Proc Natl Acad Sci U S A
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Hopfield, J.J.1
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Collins, New York. An imaginative description of a better future for healthcare that depends on proteomic arrays and in vivo imaging.
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Kessler A. The End of Medicine: How Silicon Valley (and Naked Mice) will Reboot your Doctor (2006), Collins, New York. An imaginative description of a better future for healthcare that depends on proteomic arrays and in vivo imaging.
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(2006)
The End of Medicine: How Silicon Valley (and Naked Mice) will Reboot your Doctor
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Kessler, A.1
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