Essential genes in the infection model of Pseudomonas aeruginosa PCR-based signature-tagged mutagenesis

Methods in Molecular Biology

Volumn 416, Issue , 2007, Pages 61-82

  Sanschagrin, François ^a   Kukavica Ibrulj, Irena ^a   Levesque, Roger C ^a  

^a NONE

Author keywords

Attenuation of virulence; Competitive index; En masse screening; Signature tagged mutagenesis

Indexed keywords

ANIMAL; ANIMAL MODEL; ARTICLE; BACTERIAL GENE; BIOLOGICAL MODEL; ESSENTIAL GENE; GENE EXPRESSION REGULATION; GENETICS; GRAM NEGATIVE INFECTION; METHODOLOGY; MOLECULAR BIOLOGY; PATHOPHYSIOLOGY; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; SITE DIRECTED MUTAGENESIS; VIRULENCE;

ANIMALS; GENES, BACTERIAL; GENES, ESSENTIAL; MODELS, ANIMAL; MODELS, BIOLOGICAL; MOLECULAR BIOLOGY; MUTAGENESIS, INSERTIONAL; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; VIRULENCE;

BACTERIA (MICROORGANISMS); PSEUDOMONAS AERUGINOSA; RATTUS;

EID: 40549088205     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Article

References (26)

1. Handfield, M., and Levesque, R. C. (1999) Strategies for isolation of in vivo expressed genes from bacteria. FEMS Microbiol. Rev. 23, 69-91.
2. Levesque, R. C. (2006) In Vivo Functional Genomics of Pseudomonas: PCR-Based Signature-Tagged Mutagenesis. Boston: Springer, pp. 99-120.
3. Handfield, M., Lehoux, D. E., Sanschagrin, F., Manan, M. J., Woods, D. E., and Levesque, R. C. (2000) In vivo-induced genes in Pseudomonas aeruginosa. Infect. Immun. 68, 2359-2362.
4. Hensel, M., Shea, J. E., Gleeson, C., Jones, M. D., Dalton, E., and Holden, D. W. (1995) Simultaneous identification of bacterial virulence genes by negative selection. Science 269, 400-403.
5. Lehoux, D. E., Sanschagrin, F., and Levesque, R. C. (1999) Defined oligonucleotide tag pools and PCR screening in signature-tagged mutagenesis of essential genes from bacteria. Biotechniques 26, 473-478, 480.
6. Autret, N., and Charbit, A. (2005) Lessons from signature-tagged mutagenesis on the infectious mechanisms of pathogenic bacteria. FEMS Microbiol. Rev. 29, 703-717.
7. Potvin, E., Lehoux, D. E., Kukavica-Ibrulj, I., Richard, K. L., Sanschagrin, F., Lau, G. W., and Levesque, R. C. (2003) In vivo functional genomics of Pseudomonas aeruginosa for high-throughput screening of new virulence factors and antibacterial targets. Environ. Microbiol. 5, 1294-1308.
8. Kukavica-Ibrulj, I., Peterson, A., Sanschagrin, F., Whiteley, M., and Levesque, R. C. (2006) Functional genomics of PycR, a major LysR family transcriptional regulator essential for maintenance of Pseudomonas aeruginosa in the lung. Microbiology, accepted, in revision.
9. Stover, C. K., Pham, X. Q., Erwin, A. L., Mizoguchi, S. D., Warrener, P., Hickey, M. J., et al. (2000) Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature 406, 959-964.
10. Winsor, G. L., Lo, R., Sui, S. J., Ung, K. S., Huang, S., Cheng, D., et al. (2005) Pseudomonas aeruginosa Genome Database and PseudoCAP: facilitating community-based, continually updated, genome annotation. Nucleic Acids Res. 33, D338-343.
11. Wiehlmann, L., Salunkhe, P., Larbig, K., Ritzka, R., and Tummler, B. (2002) Signaturetagged mutagenesis of Pseudomonas aeruginosa. Genome Lett. 1, 131-139.
12. Lehoux, D. E., Sanschagrin, F., and Levesque, R. C. (2001) Discovering essential and infection-related genes. Curr. Opin. Microbiol. 4, 515-519.
13. De Lorenzo, V., Herrero, M., Jakubzik, U., and Timmis, K. N. (1990) Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative eubacteria. J. Bacteriol. 172, 6568-6572.
14. Matthysse, A. G., Stretton, S., Dandie, C., McClure, N. C., and Goodman, A. E. (1996) Construction of GFP vectors for use in gram-negative bacteria other than Escherichia coli. FEMS Microbiol. Lett. 145, 87-94.
15. Choi, K. H., and Schweizer, H. P. (2005) An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants. BMC Microbiol. 5, 30.
16. Schweizer, H. P. (1991) Improved broad-host-range lac-based plasmid vectors for the isolation and characterization of protein fusions in Pseudomonas aeruginosa. Gene 103, 87-92.
17. Dewar, K., Sabbagh, L., Cardinal, G., Veilleux, F., Sanschagrin, F., Birren, B., and Levesque, R. C. (1998) Pseudomonas aeruginosa PAO1 bacterial artificial chromosomes: strategies for mapping, screening, and sequencing 100kb loci of the 5.9Mb genome. Microb. Comp. Genomics 3, 105-117.
18. Kwok, S., Kellogg, D. E., McKinney, N., Spasic, D., Goda, L., Levenson, C., and Sninsky, J. J. (1990) Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies. Nucleic Acids Res. 18, 999-1005.
19. Rychlik, W. (1993) Selection of primers for polymerase chain reaction. Totowa, NJ: Humana Press.
20. Sambrook, J., and Russell, D. W. (2001) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
21. Simon, R., Priefer, U., and Pühler, A. (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology 1, 784-791.
22. Cash, H. A., Woods, D. E., McCullough, B., Johanson, W. G. Jr., and Bass, J. A. (1979) A rat model of chronic respiratory infection with Pseudomonas aeruginosa. Am. Rev. Respir. Dis. 119, 453-459.
23. van Heeckeren, A. M., and Schluchter, M. D. (2002) Murine models of chronic Pseudomonas aeruginosa lung infection. Lab. Anim. 36, 291-312.
24. Schweizer, H. P. (1992) Allelic exchange in Pseudomonas aeruginosa using novel Co1E1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker. Mol. Microbiol. 6, 1195-1204.
25. Beuzon, C. R., and Holden, D. W. (2001) Use of mixed infections with Salmonella strains to study virulence genes and their interactions in vivo. Microbes Infect. 3, 1345-1352.
26. Hava, D. L., and Camilli, A. (2002) Large-scale identification of serotype 4 Streptococcus pneumoniae virulence factors. Mol. Microbiol. 45, 1389-1406.