Dynamitin mutagenesis reveals protein-protein interactions important for dynactin structure

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Volumn 9, Issue 4, 2008, Pages 481-491

  Maier, Kerstin C ^a   Godfrey, Jamie E ^a   Echeverri, Christophe J ^b,c   Cheong, Frances K Y ^a   Schroer, Trina A ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^c Cenix BioScience GmbH   (Germany)

Author keywords

Dynactin; Dynein; Microtubule; Motility; Motor

Indexed keywords

DYNACTIN; DYNAMITIN; GAG PROTEIN; PROTEIN P50; PROTEIN SUBUNIT; UNCLASSIFIED DRUG;

ALPHA HELIX; AMINO TERMINAL SEQUENCE; ARTICLE; CELL MOTILITY; COMPLEX FORMATION; CONTROLLED STUDY; CORRELATION FUNCTION; DIMERIZATION; GENE MUTATION; HUMAN; HUMAN CELL; IN VITRO STUDY; MICROTUBULE ASSEMBLY; MUTAGENESIS; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE;

EID: 40449083576     PISSN: 13989219     EISSN: 16000854     Source Type: Journal    
DOI: 10.1111/j.1600-0854.2008.00702.x     Document Type: Article

References (28)

1. 1 Echeverri CJ, Paschal BM, Vaughan KT, Vallee RB. Molecular characterization of 50kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis. J Cell Biol 1996 ; 132: 617–633.
2. 2 Melkonian KA, Maier KC, Godfrey JE, Rodgers M, Schroer TA. Mechanism of dynamitin‐mediated disruption of dynactin. J Biol Chem 2007 ; 282: 19355–19364.
3. 3 Eckley DM, Gill SR, Melkonian KA, Bingham JB, Goodson HV, Heuser JE, Schroer TA. Analysis of dynactin subcomplexes reveals a novel actin‐related protein associated with the Arp1 filament pointed end. J Cell Biol 1999 ; 147: 307–319.
4. 4 Clark SW, Rose MD. Arp10p is a pointed‐end‐associated component of yeast dynactin. Mol Biol Cell 2006 ; 17: 738–748.
5. 5 Imai H, Narita A, Schroer TA, Maeda Y. Two‐dimensional averaged images of the dynactin complex revealed by single particle analysis. J Mol Biol 2006 ; 359: 833–839.
6. 6 Wolf E, Kim PS, Berger B. MultiCoil: a program for predicting two‐ and three‐stranded coiled coils. Protein Sci 1997 ; 6: 1179–1189.
7. 7 McGuffin LJ, Bryson K, Jones DT. The PSIPRED protein structure prediction server. Bioinformatics 2000 ; 16: 404–405.
8. 8 Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position‐specific gap penalties and weight matrix choice. Nucleic Acids Res 1994 ; 22: 4673–4680.
9. 9 Ward JJ, McGuffin LJ, Bryson K, Buxton BF, Jones DT. The DISOPRED server for the prediction of protein disorder. Bioinformatics 2004 ; 20: 2138–2139.
10. 10 Prilusky J, Felder CE, Zeev‐Ben‐Mordehai T, Rydberg EH, Man O, Beckmann JS, Silman I, Sussman JL. FoldIndex: a simple tool to predict whether a given protein sequence is intrinsically unfolded. Bioinformatics 2005 ; 21: 3435–3438.
11. 11 Valetti C, Wetzel DM, Schrader M, Hasbani MJ, Gill SR, Kreis TE, Schroer TA. Role of dynactin in endocytic traffic: effects of dynamitin overexpression and colocalization with CLIP‐170. Mol Biol Cell 1999 ; 10: 4107–4120.
12. 12 Cleveland DW. Peptide mapping in one dimension by limited proteolysis of sodium dodecyl sulfate‐solubilized proteins. Methods Enzymol 1983 ; 96: 222–229.
13. 13 Yue L, Lu S, Garces J, Jin T, Li J. Protein kinase C‐regulated dynamitin‐macrophage‐enriched myristoylated alanine‐rice C kinase substrate interaction is involved in macrophage cell spreading. J Biol Chem 2000 ; 275: 23948–23956.
14. 14 Burkhardt JK, Echeverri CJ, Nilsson T, Vallee RB. Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dynein‐dependent maintanance of membrane organelle distribution. J Cell Biol 1997 ; 139: 469–484.
15. 15 Burkhardt JK. The role of microtubule‐based motor proteins in maintaining the structure and function of the Golgi complex. Biochim Biophys Acta 1998 ; 1404: 113–126.
16. 16 Scheich C, Niesen FH, Seckler R, Bussow K. An automated in vitro protein folding screen applied to a human dynactin subunit. Protein Sci 2004 ; 13: 370–380.
17. 17 De Bernardez Clark E, Schwarz E, Rudolph R. Inhibition of aggregation side reactions during in vitro protein folding. Methods Enzymol 1999 ; 309: 217–236.
18. 18 Middelberg AP. Preparative protein refolding. Trends Biotechnol 2002 ; 20: 437–443.
19. 19 Schafer DA, Gill SR, Cooper JA, Heuser JE, Schroer TA. Ultrastructural analysis of the dynactin complex: an actin‐related protein is a component of a filament that resembles f‐actin. J Cell Biol 1994 ; 126: 403–412.
20. 20 Starr DA, Williams BC, Hays TS, Goldberg ML. ZW10 helps recruit dynactin and dynein to the kinetochore. J Cell Biol 1998 ; 142: 763–774.
21. 21 Eckley DM, Schroer TA. Interactions between the evolutionarily conserved, actin‐related protein, Arp11, protein, actin and Arp1. Mol Biol Cell 2003 ; 14: 2645–2654.
22. 22 Quintyne NJ, Gill SR, Eckley DM, Crego CL, Compton DA, Schroer TA. Dynactin is required for microtubule anchoring at fibroblast centrosomes. J Cell Biol 1999 ; 147: 321–334.
23. 23 Wittman T, Hyman A. Recombinant p50/Dynamitin as a Tool to Examine the Role of Dynactin in Intracellular Processes. San Diego: Academic Press, 1999.
24. 24 Cohn EJ, Edsall JT. Chapter 16: density and apparent specific volume of proteins. In: Proteins, Amino Acids and Peptides as Ions and Dipolar Ions. New York: Rheinhold ; 1943, pp. 370–377.
25. 25 Laue TM, Shah BD, Ridgeway TM, Pelletier SL. Computer‐aided interpretation of analytical sedimentation data for proteins. In: Harding S, Rowe A, editors. Analytical Ultracentrifugation in Biochemistry and Polymer Science. Cambridge: Royal Society of Chemistry ; 1992, pp. 90–125.
26. 26 Hayes D, Laue T, Philo J. Sedimentation interpretation (SENDTERP). In. 1.06 ed. URL http://www.jphilo.mailway.com/. (downloaded April, 2004).
27. 27 Laemmli UK. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 1970 ; 227: 680–685.
28. 28 Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 1979 ; 76: 4350–4354.