New planar substrates for the in situ synthesis of peptide arrays

Molecular Diversity

Volumn 8, Issue 3, 2004, Pages 189-195

  Zander, Norbert ^a  

^a AIMS Scientific Products GmbH   (Germany)

Author keywords

cellulose membrane; peptide arrays; planar support; polypropylene membrane; SPOT synthesis

Indexed keywords

MACROGOL; PEPTIDE; POLYPROPYLENE; TRIFLUOROACETIC ACID;

ANTIGEN BINDING; AQUEOUS SOLUTION; ARTICLE; BILAYER MEMBRANE; ENZYME ASSAY; ENZYME PHOSPHORYLATION; MOLECULAR STABILITY; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN MICROARRAY; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

INDICATORS AND REAGENTS; MICROSCOPY, FLUORESCENCE; OLIGONUCLEOTIDES; PEPTIDES; PROTEIN ARRAY ANALYSIS; PROTEINS;

EID: 4043182780     PISSN: 13811991     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:MODI.0000036253.04738.ac     Document Type: Article

References (12)

1. Frank, R., Spot-Synthesis: An easy technique for the positionally addressable, parallel chemical synthesis on a membrane support, Tetrahedron, 48 (1992) 9217-9232.
2. Frank, R. and Overwin, H., SPOT Synthesis, In Morris, G.E. (Ed.) Epitope mapping protocols, Methods in molecular biology, Humana Press, Totowa, New Jersey, 1996, pp. 149-169.
3. All modified poylpropylene or cellulose membranes were manufactured by AIMS Scientific Products GmbH, Braunschweig, Germany (www.aims-sci.com).
4. Wenschuh, H., Volkmer-Engert, R., Schmidt, M., Schulz, M., Schneider-Mergener, J. and Reineke, U., Coherent membrane supports for parallel microsynthesis and screening of bioactive peptides, Biopolymers, 55 (2000) 188-206.
5. Daniels, S.B., Bernatowicz, M.S., Coull, J.M. and Köster, H., Membranes as solid supports for peptide synthesis, Tetrahedron Lett., 30 (1989) 4345-4348.
6. Bray, A.M., Maeji, N.J. and Geysen, H.M., The simultaneous multiple production of solution phase peptides; assessment of the Geysen method of simultaneous peptide synthesis, Tetrahedron Lett., 31 (1990) 5811-5814.
7. Chao, H.-G., Leiting, B., Reiss, P.D., Burkhardt, A.L., Klimas, C.E., Bolen, J.B. and Matsueda, G.R., Synthesis and application of Fmoc-O-[bis(dimethylamino)phosphono]-tyrosine, a versatile protected phosphotyrosine equivalent, J. Org. Chem., 60 (1995) 7710-7711.
8. Analytical reversed-phase HPLC was performed using a 50 mm x 2 mm i.d. 3 μ C18(2) column Phenomenex LUNA (Aschaffenburg, Germany) together with a Merck LaChrom HPLC system (Darmstadt, Germany). Solvent A was 0.1% TFA in HPLC-grade water, solvent B was 0.1% TFA in HPLC-grade acetonitrile. UV-detection was at 220 nm, flow rate was 0.8 mL/min. The gradient elution was: 0 min 95% A, 0-11 min 5% A, 11-12.5 min 5% A, 12.5-13 min 95% A, 13.5-17 min 95% A. HPLC-MS was performed using a 250 mm × 2 mm i.d. 5 μm C18 column Macherey & Nagel CC250/2 Nucleosil 100-5 (Düren, Germany) together with a Agilent 1100 Series HPLC system (Böblingen, Germany) and a PE Sciex API100 mass detector. Solvent A was 0.1% TFA in HPLC-grade water, solvent B was 0.1% TFA in HPLC-grade acetonitrile. UV-detection was at 214 nm, flow rate was 0.3 mL/min. The gradient elution was: 0 min 95% A, 0-20 min 5% A, 20-30 min 5% A, 30-31 min 95% A, 31-37 min 95% A.
9. Zander, N., Dittrich, F., Michaelis, K., Tegge, W. and Frank, R., A new high-performance polypropylene-based membrane support for the parallel synthesis of peptides and small organic compounds, In Benedetti, E. and Pedone, C. (Eds.), Peptides 2002 (Proceeding of the 27th European Peptide Symposium), Edizioni Ziino, Napoli, 2002, pp. 340-341.
10. Volkmer-Engert, R., Hoffmann, B. and Schneider-Mergener, J., Stable attachment of the HMB-linker to continous cellulose membranes for parallel solid phase spot synthesis, Tetrahedron Lett., 38 (1997) 1029-1032.
11. Ast, T., Heine, N., Germeroth, L., Schneider-Mergener, J. and Wenschuh, H., Efficient assambly of peptomers on continous surfaces, Tetrahedron Lett., 40 (1999) 4317-4318.
12. Zander, N., Gerhard, J. and Frank, R., Polystyrylsulfonyl-3-nitro-1H-1,2, 4-triazolide-resin: A new solid-supported reagent for the esterification of amino acids, Tetrahedron Lett., 44 (2003) 6557-6560.