Cytometry-acquired calcium-flux data analysis in activated lymphocytes

Cytometry Part A

Volumn 73, Issue 3, 2008, Pages 246-253

  Kaposi, A S ^a   Veress, G ^b   Vasarhelyi B ^a   Macardle, P ^c   Bailey, S ^c   Tulassay, T ^a   Treszl, Andras ^a  

^a SEMMELWEIS UNIVERSITY   (Hungary)

^b Analytix Ltd   (Hungary)

^c FLINDERS UNIVERSITY   (Australia)

Author keywords

Activation; Calcium flux; Data analysis; Flow cytometry; Hormesis function; Jurkat cells; T lymphocytes

Indexed keywords

CALCIUM; CD3 ANTIBODY; FLUORESCENT DYE; PHYTOHEMAGGLUTININ; THAPSIGARGIN;

ALGORITHM; ARTICLE; CALCIUM BINDING; CALCIUM TRANSPORT; CALCULATION; CD4+ T LYMPHOCYTE; COMPARATIVE STUDY; CONCENTRATION (PARAMETERS); CYTOMETRY; FLOW CYTOMETRY; HORMESIS; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; LYMPHOCYTE ACTIVATION; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; STATISTICAL ANALYSIS;

ADULT; CALCIUM; FLOW CYTOMETRY; HUMANS; LEUKOCYTES, MONONUCLEAR; LYMPHOCYTE ACTIVATION; LYMPHOCYTES;

EID: 40149097950     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20518     Document Type: Article

References (22)

1. Vereb G, Feuerstein BG, Hyun WC, Fulwyler MJ, Malazs M, Szollosi J. Biphasic calcium response of platelet-derived growth factor stimulated glioblastoma cells is a function of cell confluence. Cytometry Part A 200567A:172-179.
2. Weir IE, Maddumage R, Allan AC, Ferguson IB. Flow cytometric analysis of tracheary element differentiation in Zinnia elegans cells. Cytometry Part A 200568A:81-91.
3. Rethi B, Detre C, Gogolak P, Kolonics A, Magocsi M, Rajnavolgyi E. Flow cytometry used for the analysis of calcium signaling induced by antigen-specific T-cell activation. Cytometry 2002;47:207-216.
4. Nel AE, Slaughter N. T-cell activation through the antigen receptor, part 2: role of signaling cascades in T-cell differentiation, anergy, immune senescence, and development of immunotherapy. J Allergy Clin Immunol 2002;109:901-915.
5. Carroll HP, McNaull BB, Gadina M. Immunodeficiency is a tough nut to CRAC: The importance of calcium flux in T cell activation. Mol Interv 2006;6:253-256.
6. 2+ indicators with greatly improved fluorescence properties. J Biol Chem 1985;260:3440-3450.
7. Minta A, Kao JP, Tsien RY. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J Biol Chem 1989;264:8171-8178.
8. Novak EJ, Rabinovitch PS. Improved sensitivity in flow cytometric intracellular ionized calcium measurement using fluo-3/Fura Red fluorescence ratios. Cytometry 1994;17:135-141.
9. Rabinovitch PS, June CH, Grossmann A, Ledbetter JA. Heterogeneity among T cells in intracellular free calcium responses after mitogen stimulation with PHA-P or anti-CD3. Simultaneous use of indo-1 and immunofluorescence with flow cytometry. J Immunol 1986;137:952-961.
10. FCS Press Version 1.4b. Available at: www.fcspress.com. Accessed 1 Nov 2007.
11. FlowJo Version 8.3.3. Available at: www.flowjo.com. Accessed 1 Nov 2007.
12. R Development Core Team. R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing; 2006. ISBN 3-900051-07-0.
13. Rossini AJ, Wan JY, Le Meur N, Moodie Z.Rflowcyt: Statistical tools and data structures for analytic flow cytometry. R package version 1.5.4. Available at: http://bioconductor.org/packages/bioc/1.8/html/rflowcyt.html. Accessed 1 Nov 2007.
14. Bailey S, Macardle PJ. A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux. J Immunol Methods 2006;311:220-225.
15. June CH, Moore JS. Measurement of intracellular ions by flow cytometry. Curr Protocols Immunol 2004;1:5.5.1-5.5.20.
16. Shui JW, Boomer JS, Han J, Xu J, Dement GA, Zhou G, Tan TH. Hematopoietic progenitor kinase 1 negatively regulates T cell receptor signaling and T cell-mediated immune responses. Nat Immunol 2007;8:84-91.
17. Lamoureux J, Stankova J, Rola-Pleszczynski M. Leukotriene D4 enhances immunoglobulin production in CD40-activated human B lymphocytes. J Allergy Clin Immunol 2006;117:924-930.
18. Bridge PD, Sawilowsky SS. Increasing physicians' awareness of the impact of statistics on research outcomes: Comparative power of the t-test and and Wilcoxon Rank-Sum test in small samples applied research. J Clin Epidemiol 1999;52:229-235.
19. Calabrese EJ, Baldwin LA. Defining hormesis. Hum Exp Toxicol 2002;21:91-97.
20. Rozman KK, Doull J. Scientific foundations of hormesis, part 2: maturation, strengths, limitations, and possible applications in toxicology, pharmacology, and epidemiology. Crit Rev Toxicol 2003;33:451-462.
21. Xu S, Huo J, Tan JE, Lam KP. Cbp deficiency alters Csk localization in lipid rafts but does not affect T-cell development. Mol Cell Biol 2005;25:8486-8495.
22. Yi JS, Holbrook BC, Michalek RD, Laniewski NG, Grayson JM. Electron transport complex I is required for CD8+ T cell function. J Immunol 2006;177:852-862.