A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells

Journal of Microbiological Methods

Volumn 72, Issue 3, 2008, Pages 321-325

  Cockrell, Diane C ^a   Beare, Paul A ^a   Fischer, Elizabeth R ^b   Howe, Dale ^a   Heinzen, Robert A ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b Research Technologies Branch   (United States)

Author keywords

Cell lysis; Coxiella; Digitonin; Infectivity; Intracellular parasite; Purification; Q fever; Sonication; Vacuole

Indexed keywords

DIGITONIN;

ARTICLE; CENTRIFUGATION; COXIELLA BURNETII; CYTOLYSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PURIFICATION; TRANSMISSION ELECTRON MICROSCOPY; ULTRASOUND;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; CELL MEMBRANE; CERCOPITHECUS AETHIOPS; COXIELLA BURNETII; DIGITONIN; MICROSCOPY; MICROSCOPY, ELECTRON, TRANSMISSION; VERO CELLS;

BACTERIA (MICROORGANISMS); COXIELLA; COXIELLA BURNETII;

EID: 39949085413     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2007.12.015     Document Type: Article

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