An automated system for intracellular and intranuclear injection

Journal of Neuroscience Methods

Volumn 169, Issue 1, 2008, Pages 65-75

  Hogg, Ron C ^a   Bandelier, Florence ^a   Benoit, Audrey ^a   Dosch, Roland ^a   Bertrand, Daniel ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

Author keywords

Drug screening; Electrophysiology; Intranuclear injection; Lab automation; RNA injection; Xenopus oocyte; Zebrafish

Indexed keywords

COMPLEMENTARY DNA; ION CHANNEL;

ARTICLE; AUTOMATION; COMPUTER AIDED DESIGN; CONTROLLED STUDY; ELECTROPHYSIOLOGY; NONHUMAN; OOCYTE; PRIORITY JOURNAL; VALIDATION STUDY; XENOPUS; ZEBRA FISH;

ANIMALS; AUTOMATION; BIOLOGICAL ASSAY; CELL CULTURE TECHNIQUES; CELL NUCLEUS; CYTOLOGICAL TECHNIQUES; DNA, COMPLEMENTARY; DRUG EVALUATION, PRECLINICAL; FEMALE; ION CHANNELS; MICROINJECTIONS; OOCYTES; RNA, MESSENGER; XENOPUS LAEVIS; ZEBRAFISH;

EID: 39749112484     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2007.11.028     Document Type: Article

References (31)

1. Berghmans S., Hunt J., Roach A., and Goldsmith P. Zebrafish offer the potential for a primary screen to identify a wide variety of potential anticonvulsants. Epilepsy Res 75 (2007) 18-28
2. Bertrand D., Cooper E., Valera S., Rungger D., and Ballivet M. Electrophysiology of neuronal nicotinic acetylcholine receptors expressed in Xenopus oocytes following nuclear injection of genes or cDNA. Methods Neurosci 4 (1991) 174-183
3. Brand M., Granato M., and Nüsslein-Volhard C. In: Nüsslein-Volhard C., and Dahm R. (Eds). Keeping and raising zebrafish (2002), Zebrafish Oxford University Press, Oxford 7-37
4. Burns C.G., Milan D.J., Grande E.J., Rottbauer W., MacRae C.A., and Fishman M.C. High-throughput assay for small molecules that modulate zebrafish embryonic heart rate. Nat Chem Biol 1 (2005) 263-264
5. Gamarnik A.V., and Andino R. Replication of poliovirus in Xenopus oocytes requires two human factors. EMBO J 15 (1996) 5988-5998
6. Gamarnik A.V., and Andino R. Exploring RNA virus replication in Xenopus oocytes. Methods Mol Biol 322 (2006) 367-378
7. Gamarnik A.V., Boddeker N., and Andino R. Translation and replication of human rhinovirus type 14 and mengovirus in Xenopus oocytes. J Virol 74 (2000) 11983-11987
8. Gurdon J.B., Lane C.D., Woodland H.R., and Marbaix G. Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. Nature 233 (1971) 177-182
9. Gurdon J.B., Lingrel J.B., and Marbaix G. Message stability in injected frog oocytes: long life of mammalian alpha and beta globin messages. J Mol Biol 80 (1973) 539-551
10. Gurdon J.B., Woodland H.R., and Lingrel J.B. The translation of mammalian globin mRNA injected into fertilized eggs of Xenopus laevis. I. Message stability in development. Dev Biol 39 (1974) 125-133
11. Kimmel C.B., Ballard W.W., Kimmel S.R., Ullmann B., and Schilling T.F. Stages of embryonic development of the zebrafish. Dev Dyn 203 (1995) 253-310
12. Laskey R.A., and Gurdon J.B. The translation of viral RNAs in frog oocytes. Hamatol Bluttransfus 14 (1974) 272-274
13. Laskey R.A., Gurdon J.B., and Crawford L.V. Translation of encephalomyocarditis viral RNA in oocytes of Xenopus laevis. Proc Natl Acad Sci USA 69 (1972) 3665-3669
14. Lewis T.M., Harkness P.C., Sivilotti L.G., Colquhoun D., and Millar N.S. The ion channel properties of a rat recombinant neuronal nicotinic receptor are dependent on the host cell type. J Physiol 505 Pt. 2 (1997) 299-306
15. Lieschke G.J., and Currie P.D. Animal models of human disease: zebrafish swim into view. Nat Rev Genet 8 (2007) 353-367
16. Marsal J., Tigyi G., and Miledi R. Incorporation of acetylcholine receptors and Cl-channels in Xenopus oocytes injected with Torpedo electroplaque membranes. Proc Natl Acad Sci USA 92 (1995) 5224-5228
17. Miledi R., Parker I., and Sumikawa K. Properties of acetylcholine receptors translated by cat muscle mRNA in Xenopus oocytes. EMBO J 1 (1982) 1307-1312
18. Miledi R., Parker I., and Sumikawa K. Synthesis of chick brain GABA receptors by frog oocytes. Proc R Soc Lond B: Biol Sci 216 (1982) 509-515
19. Miledi R., Parker I., and Sumikawa K. Recording of single gamma-aminobutyrate- and acetylcholine-activated receptor channels translated by exogenous mRNA in Xenopus oocytes. Proc R Soc Lond B: Biol Sci 218 (1983) 481-484
20. Miledi R., Eusebi F., Martinez-Torres A., Palma E., and Trettel F. Expression of functional neurotransmitter receptors in Xenopus oocytes after injection of human brain membranes. Proc Natl Acad Sci USA 99 (2002) 13238-13242
21. Miledi R., Palma E., and Eusebi F. Microtransplantation of neurotransmitter receptors from cells to Xenopus oocyte membranes: new procedure for ion channel studies. Methods Mol Biol 322 (2006) 347-355
22. Morales A., Aleu J., Ivorra I., Ferragut J.A., Gonzalez-Ros J.M., and Miledi R. Incorporation of reconstituted acetylcholine receptors from Torpedo into the Xenopus oocyte membrane. Proc Natl Acad Sci USA 92 (1995) 8468-8472
23. Palma E., Trettel F., Fucile S., Renzi M., Miledi R., and Eusebi F. Microtransplantation of membranes from cultured cells to Xenopus oocytes: a method to study neurotransmitter receptors embedded in native lipids. Proc Natl Acad Sci USA 100 (2003) 2896-2900
24. Romero M.F., Kanai Y., Gunshin H., and Hediger M.A. Expression cloning using Xenopus laevis oocytes. Methods Enzymol 296 (1998) 17-52
25. Sakai T., Tochio H., Tenno T., Ito Y., Kokubo T., Hiroaki H., et al. In-cell NMR spectroscopy of proteins inside Xenopus laevis oocytes. J Biomol NMR 36 (2006) 179-188
26. Selenko P., and Wagner G. Looking into live cells with in-cell NMR spectroscopy. J Struct Biol 158 (2007) 244-253
27. Selenko P., Serber Z., Gadea B., Ruderman J., Wagner G., and Quantitative. NMR analysis of the protein G B1 domain in Xenopus laevis egg extracts and intact oocytes. Proc Natl Acad Sci USA 103 (2006) 11904-11909
28. Sivilotti L.G., McNeil D.K., Lewis T.M., Nassar M.A., Schoepfer R., and Colquhoun D. Recombinant nicotinic receptors, expressed in Xenopus oocytes, do not resemble native rat sympathetic ganglion receptors in single-channel behaviour. J Physiol 500 Pt. 1 (1997) 123-138
29. In: Westerfield M. (Ed). The zebrafish book: a guide for the laboratory use of zebrafish (Danio rerio). 4th ed. (2000), University of Oregon Press, Eugene
30. Wickens M.P., Woo S., O'Malley B.W., and Gurdon J.B. Expression of a chicken chromosomal ovalbumin gene injected into frog oocyte nuclei. Nature 285 (1980) 628-634
31. Woodland H.R., Gurdon J.B., and Lingrel J.B. The translation of mammalian globin mRNA injected into fertilized eggs of Xenopus laevis. II. The distribution of globin synthesis in different tissues. Dev Biol 39 (1974) 134-140