Selected non-ionic biological detergents enhance signal intensity of intact bovine serum albumin by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

European Journal of Mass Spectrometry

Volumn 13, Issue 5, 2007, Pages 311-319

  Brinkworth, Craig S ^a   Bourne, David J ^a  

^a DEFENCE SCIENCE AND TECHNOLOGY ORGANISATION   (Australia)

Author keywords

BSA; Intact protein; MALDI MS; Non ionic detergents; Sensitivity

Indexed keywords

BOVINE SERUM ALBUMIN; DEOXYCHOLIC ACID; DETERGENT; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; MACROGOL DERIVATIVE; POLYSORBATE;

ARTICLE; CHEMISTRY; MASS SPECTROMETRY; SOLUTION AND SOLUBILITY;

DEOXYCHOLIC ACID; DETERGENTS; INDICATORS AND REAGENTS; POLYETHYLENE GLYCOLS; POLYSORBATES; SERUM ALBUMIN, BOVINE; SOLUTIONS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 39749110082     PISSN: 14690667     EISSN: None     Source Type: Journal    
DOI: 10.1255/ejms.886     Document Type: Article

References (17)

1. M. Kussmann, M. Affolter and L.B. Fay, "Proteomics in nutrition and health", Comb. Chem. High Throughput Screen 8, 679 (2005). doi: 10.2174/138620705774962526
2. R. J. Chalkley, K.C. Hansen and M. A. Baldwin, "Bioinformatic methods to exploit mass spectrometric data for proteomic applications", Methods Enzymol. 402, 289 (2005). doi: 10.1016/S0076-6879(05) 02009-4
3. B.J. Cargile, J.R. Sevinsky, A.S. Essader, J.L. Stephenson, Jr and J.L. Bundy, "Immobilized pH gradient isoelectric focusing as a first-dimension separation in shotgun proteomics", J. Biomol. Tech. 16, 181 (2005).
4. J. Li, A. Martin, A.D. Cox, E.R. Moxon, J.C. Richards and P. Thibault, "Mapping bacterial glycolipid complexity using capillary electrophoresis and electrospray mass spectrometry", Methods Enzymol. 405, 369 (2005). doi: 10.1016/S0076-6879(05)05013-5
5. L. Huang and A.L. Burlingame, "Comprehensive mass spectrometric analysis of the 20S proteasome complex", Methods Enzymol. 405, 187 (2005). doi: 10.1016/S0076-6879(05)05009-3
6. C.S. Jorgensen, M. Jagd, B.K. Sorensen, J. McGuire, V. Barkholt, P. Hojrup and G. Houen, "Efficacy and compatibility with mass spectrometry of methods for elution of proteins from sodium dodecyl sulfate-polyacrylamide gels and poly-vinyldifluoride membranes", Anal. Biochem. 330, 87 (2004). doi: 10.1016/j.ab.2004.03.012
7. D. Drexler, D.J. Barlow, P. Falk, J. Cantone, D. Hernandez, A. Ranasinghe, M. Sanders, B. Warrack and F. McPhee, "Development of an on-line automated sample clean-up method and liquid chromatography-tandem mass spectrometry analysis: application in an in vitro proteolytic assay", Anal. Bioanal. Chem. 384, 1145 (2006). doi: 10.1007/s00216-005- 0263-7
8. K.O. Bornsen, "Influence of salts, buffers, detergents, solvents, and matrices on MALDI-MS protein analysis in complex mixtures", Methods Mol. Biol. 146, 387 (2000).
9. C. Fenselau, "MALDI MS and strategies for protein analysis",Anal. Chem. 69, 661A (1997).
10. M. Kussmann and P. Roepstorff, "Sample preparation techniques for peptides and proteins analyzed by MALDI-MS", Methods Mol. Biol. 146, 405 (2000).
11. J. Zheng, N. Li, M. Ridyard, H. Dai, S.M. Robbins and L. Li, "Simple and robust two-layer matrix/sample preparation method for MALDI MS/MS analysis of peptides", J. Proteome Res. 4, 1709 (2005). doi: 10.1021/pr050157w
12. X. Chen, A. Murawski, G. Kuang, D.J. Sexton and W. Galbraith, "Sample preparation for MALDI mass spectrometry using an elastomeric device reversibly sealed on the MALDI target", Anal. Chem. 78, 6160 (2006). doi: 10.1021/ac060286b
13. F. Gharahdaghi, M. Kirchner, J. Fernandez and S.M. Mische, "Peptide-mass profiles of polyvinylidene difluoride-bound proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in the presence of nonionic detergents", Anal. Biochem. 233, 94 (1996). doi: 10.1006/abio.1996.0012
14. R.V. Rajnarayanan and K. Wang, "Ion-pair assisted recovery of matrix-assisted laser desorption/ionization mass spectral signals from SDS-containing peptide-protein mixtures", J. Mass Spectrom. 39, 79 (2004). doi: 10.1002/jms.572
15. S. Fukuzawa, M. Asanuma, K. Tachibana and H. Hirota, "On-probe sample preparation without washes for matrix-assisted laser desorption/ ionization mass spectrometry using an anion exchange medium", Anal. Chem. 77, 5750 (2005). doi: 10.1021/ac0500129
16. K.O. Bornsen, M.A. Gass, G.J. Bruin, J.H. von Adrichem, M.C. Biro, G.M. Kresbach and M. Ehrat, "Influence of solvents and detergents on matrix-assisted laser desorption/ionization mass spectrometry measurements of proteins and oligonucleotides", Rapid Commun. Mass Spectrom. 11, 603 (1997). doi: 10.1002/(SICI)1097-0231(199704)11:6<603:: AID-RCM879>3.0.CO;2-U
17. C.S. Brinkworth and D.J. Bourne, "The effect of Tween80 on signal intensity of intact proteins by MALDI time-of-flight mass spectrometry", J. Am. Soc. Mass Spectrom. 18, 102 (2007). doi: 10.1016/j.jasms.2006.09.003