In vitro expansion affects the response of chondrocytes to mechanical stimulation

Osteoarthritis and Cartilage

Volumn 16, Issue 3, 2008, Pages 385-391

  Das, R H J ^a   Jahr, H ^a   Verhaar, J A N ^a   van der Linden, J C ^a,b   van Osch, G J V M ^a   Weinans, H ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

^b DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

Cell culture; Cell deformation; Chondrocyte phenotype; Dedifferentiation; Gene expression; Mechanical loading; Stretch; Tissue engineering

Indexed keywords

AGGRECAN; COLLAGEN TYPE 1; COLLAGEN TYPE 10; COLLAGEN TYPE 2; COLLAGENASE 3; INTERSTITIAL COLLAGENASE; STROMELYSIN; TRANSCRIPTION FACTOR SOX9;

ARTICLE; CARTILAGE CELL; CELL DIFFERENTIATION; CELL STIMULATION; CONTROLLED STUDY; CULTURE MEDIUM; DOWN REGULATION; GENE EXPRESSION; HUMAN; HUMAN CELL; IN VITRO STUDY; MECHANICAL STRESS; MONOLAYER CULTURE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION;

AGGRECANS; CARTILAGE, ARTICULAR; CELL DIFFERENTIATION; CELL SIZE; CELLS, CULTURED; CHONDROCYTES; COLLAGEN TYPE X; CULTURE MEDIA, CONDITIONED; DOWN-REGULATION; EXTRACELLULAR MATRIX; FIBRILLAR COLLAGENS; GENE EXPRESSION; GLYCOPROTEINS; HIGH MOBILITY GROUP PROTEINS; HUMANS; MATRIX METALLOPROTEINASES; PHENOTYPE; REGENERATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STRESS, MECHANICAL; TISSUE ENGINEERING; TRANSCRIPTION FACTORS; UP-REGULATION;

EID: 39749097208     PISSN: 10634584     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.joca.2007.07.014     Document Type: Article

References (38)

1. von der Mark K., Gauss V., von der Mark H., and Muller P. Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture. Nature 267 (1977) 531-532
2. Mandl E.W., van der Veen S.W., Verhaar J.A., and van Osch G.J. Multiplication of human chondrocytes with low seeding densities accelerates cell yield without losing redifferentiation capacity. Tissue Eng 10 (2004) 109-118
3. Schnabel M., Marlovits S., Eckhoff G., Fichtel I., Gotzen L., Vecsei V., et al. Dedifferentiation-associated changes in morphology and gene expression in primary human articular chondrocytes in cell culture. Osteoarthritis Cartilage 10 (2002) 62-70
4. Guilak F., Ratcliffe A., and Mow V.C. Chondrocyte deformation and local tissue strain in articular cartilage: a confocal microscopy study. J Orthop Res 13 (1995) 410-421
5. Sah R.L., Kim Y.J., Doong J.Y., Grodzinsky A.J., Plaas A.H., and Sandy J.D. Biosynthetic response of cartilage explants to dynamic compression. J Orthop Res 7 (1989) 619-636
6. Larsson T., Aspden R.M., and Heinegard D. Effects of mechanical load on cartilage matrix biosynthesis in vitro. Matrix 11 (1991) 388-394
7. Kim Y.J., Sah R.L., Grodzinsky A.J., Plaas A.H., and Sandy J.D. Mechanical regulation of cartilage biosynthetic behavior: physical stimuli. Arch Biochem Biophys 311 (1994) 1-12
8. Buschmann M.D., Gluzband Y.A., Grodzinsky A.J., and Hunziker E.B. Mechanical compression modulates matrix biosynthesis in chondrocyte/agarose culture. J Cell Sci 108 Pt 4 (1995) 1497-1508
9. Quinn T.M., Grodzinsky A.J., Buschmann M.D., Kim Y.J., and Hunziker E.B. Mechanical compression alters proteoglycan deposition and matrix deformation around individual cells in cartilage explants. J Cell Sci 111 Pt 5 (1998) 573-583
10. Lee D.A., Noguchi T., Frean S.P., Lees P., and Bader D.L. The influence of mechanical loading on isolated chondrocytes seeded in agarose constructs. Biorheology 37 (2000) 149-161
11. Bonassar L.J., Grodzinsky A.J., Frank E.H., Davila S.G., Bhaktav N.R., and Trippel S.B. The effect of dynamic compression on the response of articular cartilage to insulin-like growth factor-I. J Orthop Res 19 (2001) 11-17
12. Nugent G.E., Aneloski N.M., Schmidt T.A., Schumacher B.L., Voegtline M.S., and Sah R.L. Dynamic shear stimulation of bovine cartilage biosynthesis of proteoglycan 4. Arthritis Rheum 54 (2006) 1888-1896
13. Smith R.L., Donlon B.S., Gupta M.K., Mohtai M., Das P., Carter D.R., et al. Effects of fluid-induced shear on articular chondrocyte morphology and metabolism in vitro. J Orthop Res 13 (1995) 824-831
14. Edlich M., Yellowley C.E., Jacobs C.R., and Donahue H.J. Oscillating fluid flow regulates cytosolic calcium concentration in bovine articular chondrocytes. J Biomech 34 (2001) 59-65
15. Holmvall K., Camper L., Johansson S., Kimura J.H., and Lundgren-Akerlund E. Chondrocyte and chondrosarcoma cell integrins with affinity for collagen type II and their response to mechanical stress. Exp Cell Res 221 (1995) 496-503
16. Akagi M., Nishimura S., Yoshida K., Kakinuma T., Sawamura T., Munakata H., et al. Cyclic tensile stretch load and oxidized low density lipoprotein synergistically induce lectin-like oxidized ldl receptor-1 in cultured bovine chondrocytes, resulting in decreased cell viability and proteoglycan synthesis. J Orthop Res 24 (2006) 1782-1790
17. Millward-Sadler S.J., Wright M.O., Davies L.W., Nuki G., and Salter D.M. Mechanotransduction via integrins and interleukin-4 results in altered aggrecan and matrix metalloproteinase 3 gene expression in normal, but not osteoarthritic, human articular chondrocytes. Arthritis Rheum 43 (2000) 2091-2099
18. Salter D.M., Millward-Sadler S.J., Nuki G., and Wright M.O. Differential responses of chondrocytes from normal and osteoarthritic human articular cartilage to mechanical stimulation. Biorheology 39 (2002) 97-108
19. Salter D.M., Wright M.O., and Millward-Sadler S.J. NMDA receptor expression and roles in human articular chondrocyte mechanotransduction. Biorheology 41 (2004) 273-281
20. Millward-Sadler S.J., Wright M.O., Flatman P.W., and Salter D.M. ATP in the mechanotransduction pathway of normal human chondrocytes. Biorheology 41 (2004) 567-575
21. Plumb M.S., and Aspden R.M. The response of elderly human articular cartilage to mechanical stimuli in vitro. Osteoarthritis Cartilage 13 (2005) 1084-1091
22. Wiseman M., Bader D.L., Reisler T., and Lee D.A. Passage in monolayer influences the response of chondrocytes to dynamic compression. Biorheology 41 (2004) 283-298
23. Weyts F.A., Bosmans B., Niesing R., van Leeuwen J.P., and Weinans H. Mechanical control of human osteoblast apoptosis and proliferation in relation to differentiation. Calcif Tissue Int 72 (2003) 505-512
24. Uitterlinden E.J., Jahr H., Koevoet J.L., Jenniskens Y.M., Bierma-Zeinstra S.M., Degroot J., et al. Glucosamine decreases expression of anabolic and catabolic genes in human osteoarthritic cartilage explants. Osteoarthritis Cartilage 14 (2006) 250-257
25. Livak K.J., and Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25 (2001) 402-408
26. Zhou D., Lee H.S., Villarreal F., Teng A., Lu E., Reynolds S., et al. Differential MMP-2 activity of ligament cells under mechanical stretch injury: an in vitro study on human ACL and MCL fibroblasts. J Orthop Res 23 (2005) 949-957
27. Yang G., Im H.J., and Wang J.H. Repetitive mechanical stretching modulates IL-1beta induced COX-2, MMP-1 expression, and PGE2 production in human patellar tendon fibroblasts. Gene 363 (2005) 166-172
28. Shelton L., and Rada J.S. Effects of cyclic mechanical stretch on extracellular matrix synthesis by human scleral fibroblasts. Exp Eye Res 84 (2007) 314-322
29. Yoshida M., Sagawa N., Itoh H., Yura S., Takemura M., Wada Y., et al. Prostaglandin F(2alpha), cytokines and cyclic mechanical stretch augment matrix metalloproteinase-1 secretion from cultured human uterine cervical fibroblast cells. Mol Hum Reprod 8 (2002) 681-687
30. Tyagi S.C., Lewis K., Pikes D., Marcello A., Mujumdar V.S., Smiley L.M., et al. Stretch-induced membrane type matrix metalloproteinase and tissue plasminogen activator in cardiac fibroblast cells. J Cell Physiol 176 (1998) 374-382
31. Sambajon V.V., Cillo Jr. J.E., Gassner R.J., and Buckley M.J. The effects of mechanical strain on synovial fibroblasts. J Oral Maxillofac Surg 61 (2003) 707-712
32. Reboul P., Pelletier J.P., Tardif G., Cloutier J.M., and Martel-Pelletier J. The new collagenase, collagenase-3, is expressed and synthesized by human chondrocytes but not by synoviocytes. A role in osteoarthritis. J Clin Invest 97 (1996) 2011-2019
33. Rannou F., Francois M., Corvol M.T., and Berenbaum F. Cartilage breakdown in rheumatoid arthritis. Joint Bone Spine 73 (2006) 29-36
34. Millward-Sadler S.J., and Salter D.M. Integrin-dependent signal cascades in chondrocyte mechanotransduction. Ann Biomed Eng 32 (2004) 435-446
35. Goessler U.R., Bieback K., Bugert P., Heller T., Sadick H., Hormann K., et al. In vitro analysis of integrin expression during chondrogenic differentiation of mesenchymal stem cells and chondrocytes upon dedifferentiation in cell culture. Int J Mol Med 17 (2006) 301-307
36. Knight M.M., van de Breevaart Bravenboer J., Lee D.A., van Osch G.J., Weinans H., and Bader D.L. Cell and nucleus deformation in compressed chondrocyte-alginate constructs: temporal changes and calculation of cell modulus. Biochim Biophys Acta 1570 (2002) 1-8
37. Guilak F., and Mow V.C. The mechanical environment of the chondrocyte: a biphasic finite element model of cell-matrix interactions in articular cartilage. J Biomech 33 (2000) 1663-1673
38. Lee M.S., Trindade M.C., Ikenoue T., Schurman D.J., Goodman S.B., and Smith R.L. Effects of shear stress on nitric oxide and matrix protein gene expression in human osteoarthritic chondrocytes in vitro. J Orthop Res 20 (2002) 556-561