Employment of the mouse median nerve model for the experimental assessment of peripheral nerve regeneration

Journal of Neuroscience Methods

Volumn 169, Issue 1, 2008, Pages 119-127

  Tos, P ^a   Ronchi, G ^b   Nicolino, S ^b   Audisio, C ^b   Raimondo, S ^b   Fornaro, M ^b   Battiston, B ^a   Graziani, A ^c   Perroteau, I ^b   Geuna, S ^b  

^a CTO HOSPITAL   (Italy)

^b UNIVERSITY OF TURIN   (Italy)

^c UNIVERSITY OF EASTERN PIEDMONT   (Italy)

Author keywords

Grasping test; Median nerve repair; Mouse; Peripheral nerve regeneration; Quantitative morphology

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; CONTROLLED STUDY; ELECTRON MICROSCOPY; GRIP STRENGTH; IMMUNOHISTOCHEMISTRY; MALE; MEDIAN NERVE; MOUSE; NERVE FIBER; NERVE REGENERATION; NONHUMAN; PERIPHERAL NERVE; PRIORITY JOURNAL; RAT; SURGICAL TECHNIQUE; SUTURE;

ANIMALS; AXONS; BIOLOGICAL ASSAY; BIOLOGICAL MARKERS; DISEASE MODELS, ANIMAL; FORELIMB; HAND STRENGTH; IMMUNOHISTOCHEMISTRY; MALE; MEDIAN NERVE; MICE; MUSCLE, SKELETAL; NERVE FIBERS, MYELINATED; NERVE REGENERATION; NERVE TISSUE PROTEINS; NEUROSURGICAL PROCEDURES; PARALYSIS; RECOVERY OF FUNCTION; SUTURE TECHNIQUES;

EID: 39749084597     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2007.11.030     Document Type: Article

References (36)

1. Baptista A.F., Gomes J.R., Oliveira J.T., Santos S.M., Vannier-Santos M.A., and Martinez A.M. A new approach to assess function after sciatic nerve lesion in the mouse-adaptation of the sciatic static index. J Neurosci Methods 161 (2007) 259-264
2. Bertelli J.A., and Mira J.C. The grasping test: a simple behavioral method for objective quantitative assessment of peripheral nerve regeneration in the rat. J Neurosci Methods 58 (1995) 151-155
3. Bontioti E., Kanje M., Lundborg G., and Dahlin L.B. End-to-side nerve repair in the upper extremity of rat. J Peripher Nerv Syst 10 (2005) 58-68
4. Chalfoun C.T., Wirth G.A., and Evans G.R. Tissue engineered nerve constructs: where do we stand?. J Cell Mol Med 10 (2006) 309-317
5. Chan W.Y., Matteucci P., and Southern S.J. Validation of microsurgical models in microsurgery training and competence: a review. Microsurgery 27 (2007) 494-499
6. Fazan V.P., Ma X., Chapleau M.W., and Barreira A.A. Qualitative and quantitative morphology of renal nerves in C57BL/6J mice. Anat Rec 268 (2002) 399-404
7. Galtrey C.M., and Fawcett J.W. Characterization of tests of functional recovery after median and ulnar nerve injury and repair in the rat forelimb. J Peripher Nerv Syst 12 (2007) 11-27
8. Geuna S. The revolution of counting "tops": two decades of the disector principle in morphological research. Microsc Res Tech 66 (2005) 270-274
9. Geuna S., Tos P., Battiston B., and Guglielmone R. Verification of the two-dimensional disector, a method for the unbiased estimation of density and number of myelinated nerve fibers in peripheral nerves. Ann Anat 182 (2000) 23-34
10. Geuna S., Tos P., Guglielmone R., Battiston B., and Giacobini-Robecchi M.G. A stereological study of long-term regeneration of rat severed sciatic nerve repaired by means of muscle-vein-combined grafts. Ital J Anat Embryol 105 (2000) 65-73
11. Geuna S., Tos P., Guglielmone R., Battiston B., and Giacobini-Robecchi M.G. Methodological issues in size estimation of myelinated nerve fibers in peripheral nerves. Anat Embryol 204 (2001) 1-10
12. Geuna S., Gigo-Benato D., and Rodrigues A.C. On sampling and sampling errors in histomorphometry of peripheral nerve fibers. Microsurgery 24 (2004) 72-76
13. Geuna S., Papalia I., and Tos P. End-to-side (terminolateral) nerve regeneration: a challenge for neuroscientists coming from an intriguing nerve repair concept. Brain Res Rev 52 (2006) 381-388
14. Geuna S., Nicolino S., Raimondo S., Gambarotta G., Battiston B., Tos P., et al. Nerve regeneration along bioengineered scaffolds. Microsurgery 27 (2007) 429-438
15. Hayashi A., Koob J.W., Liu D.Z., Tong A.Y., Hunter D.A., Parsadanian A., et al. A double-transgenic mouse used to track migrating Schwann cells and regenerating axons following engraftment of injured nerves. Exp Neurol 207 (2007) 128-138
16. Islamov R.R., Hendricks W.A., Jones R.J., Lyall G.J., Spanier N.S., and Murashov A.K. 17-Beta-estradiol stimulates regeneration of sciatic nerve in female mice. Brain Res 943 (2002) 283-286
17. Kanaya F., Firrell J.C., and Breidenbach W.C. Sciatic function index, nerve conduction tests, muscle contraction, and axon morphometry as indicators of regeneration. Plast Reconstr Surg 98 (1996) 1264-1271
18. Koob J.W., Moradzadeh A., Tong A., Hayashi A., Myckatyn T.M., Tung T.H., et al. Induction of regional collateral sprouting following muscle denervation. Laryngoscope 117 (2007) 1735-1740
19. Larsen J.O. Related stereology of nerve cross sections. J Neurosci Methods 85 (1998) 107-118
20. Lee J.M., Tos P., Raimondo S., Fornaro M., Papalia I., Geuna S., et al. Lack of topographic specificity in nerve fiber regeneration of rat forelimb mixed nerves. Neuroscience 144 (2007) 985-990
21. Luis A.L., Amado S., Geuna S., Rodrigues J.M., Simoes M.J., Santos J.D., et al. Long-term functional and morphological assessment of a standardized rat sciatic nerve crush injury with a non-serrated clamp. J Neurosci Methods 163 (2007) 92-104
22. Lundborg G. Nerve injury and repair (2005), Churchill Livingstone Edinburgh, Edinburgh
23. Mackinnon S.E., Dellon A.L., and O'Brien J.P. Changes in nerve fiber numbers distal to a nerve repair in the rat sciatic nerve model. Muscle Nerve 14 (1991) 1116-1122
24. Magill C.K., Tong A., Kawamura D., Hayashi A., Hunter D.A., Parsadanian A., et al. Reinnervation of the tibialis anterior following sciatic nerve crush injury: a confocal microscopic study in transgenic mice. Exp Neurol 207 (2007) 64-74
25. Pakkenberg B., and Gundersen H.J. Neocortical neuron number in humans: effect of sex and age. J Compd Neurol 384 (1997) 312-320
26. Papalia I., Tos P., Stagno d'Alcontres F., Battiston B., and Geuna S. On the use of the grasping test in the rat median nerve model: a re-appraisal of its efficacy for quantitative assessment of motor function recovery. J Neurosci Methods 127 (2003) 43-47
27. Papalia I., Tos P., Scevola A., Raimondo S., and Geuna S. The ulnar test: a method for the quantitative functional assessment of posttraumatic ulnar nerve recovery in the rat. J Neurosci Methods 154 (2006) 198-203
28. Pereira Lopes F.R., Camargo de Moura Campos L., Dias Correa Jr. J., Balduino A., Lora S., Langone F., et al. Bone marrow stromal cells and resorbable collagen guidance tubes enhance sciatic nerve regeneration in mice. Exp Neurol 198 (2006) 457-468
29. Pfister L.A., Papaloizos M., Merkle H.P., and Gander B. Nerve conduits and growth factor delivery in peripheral nerve repair. J Peripher Nerv Syst 12 (2007) 65-82
30. Robl J.M., Wang Z., Kasinathan P., and Kuroiwa Y. Transgenic animal production and animal biotechnology. Theriogenology 67 (2007) 127-133
31. Santos A.P., Suaid C.A., Fazan V.P., and Barreira A.A. Microscopic anatomy of brachial plexus branches in Wistar rats. Anat Rec 290 (2007) 477-485
32. Schmitz C. Variation of fractionator estimates and its prediction. Anat Embryol 198 (1998) 371-397
33. Shao C., Liu M., Wu X., and Ding F. Time-dependent expression of myostatin RNA transcript and protein in gastrocnemius muscle of mice after sciatic nerve resection. Microsurgery 27 (2007) 487-493
34. Sinis N., Schaller H.E., Becker S.T., Lanaras T., Schulte-Eversum C., Muller H.W., et al. Cross-chest median nerve transfer: a new model for the evaluation of nerve regeneration across a 40 mm gap in the rat. J Neurosci Methods 156 (2006) 166-172
35. Tos P., Battiston B., Nicolino S., Raimondo S., Fornaro M., Lee J.M., et al. Comparison of fresh and predegenerated muscle-vein-combined guides for the repair of rat median nerve. Microsurgery 27 (2007) 48-55
36. Varejão A.S.P., Melo-Pinto P., Meek M.F., Filipe V.M., and Bulas-Cruz J. Methods for the experimental functional assessment of rat sciatic nerve regeneration. Neurol Res 26 (2004) 186-194