Fed-batch cultivation of the marine bacterium Sulfitobacter pontiacus using immobilized substrate and purification of sulfite oxidase by application of membrane adsorber technology

Biotechnology and Bioengineering

Volumn 99, Issue 4, 2008, Pages 870-875

  Muffler, Kai ^a   Ulber, Roland ^a  

^a Rheinland Pfälzische Technische Universität Kaiserslautern Landau   (Germany)

Author keywords

Cultivation performance; Fed batch; Immobilized substrate; Membrane adsorber; Optimization; Sulfite oxidase

Indexed keywords

BIOLOGICAL MEMBRANES; BIOSENSORS; BIOSYNTHESIS; CELL GROWTH; ENZYME IMMOBILIZATION; MARINE BIOLOGY; PURIFICATION;

CULTIVATION PERFORMANCE; FED BATCHES; GRAM NEGATIVE HETEROTROPHIC BACTERIA; IMMOBILIZED SUBSTRATES; MEMBRANE ADSORBERS; SULFITE OXIDASE;

BACTERIA;

SULFITE OXIDASE; SULFITE;

ARTICLE; BACTERIAL STRAIN; BIOREACTOR; BIOSENSOR; CAPILLARY ELECTROPHORESIS; ENZYME PURIFICATION; ENZYME SUBSTRATE; ENZYME SYNTHESIS; FED BATCH CULTURE; FERMENTATION; FOOD; MARINE BACTERIUM; MEMBRANE ADSORBER; PROTEIN DETERMINATION; SPECIES CULTIVATION; SULFITOBACTER PONTIACUS; ADSORPTION; BIOLOGY; CULTURE TECHNIQUE; GRAM NEGATIVE BACTERIUM; IMMOBILIZED CELL; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGY; SEA; ULTRAFILTRATION;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; SULFITOBACTER PONTIACUS;

ADSORPTION; BIOREACTORS; CELL CULTURE TECHNIQUES; CELLS, IMMOBILIZED; GRAM-NEGATIVE BACTERIA; MARINE BIOLOGY; OCEANS AND SEAS; SULFITE OXIDASE; SULFITES; ULTRAFILTRATION;

EID: 39549107606     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21631     Document Type: Article

References (15)

1. Baker GJ, Collett P, Allen DH. 1981. Bronchospasm induced by metabisulfite-containing foods and drugs. Med J Aust 2:614-617.
2. Hinson DL, Webber RJ. 1988. Miniaturization of the BCA protein assay. BioTechniques 6:14-19.
3. Jeude M, Dittrich B, Niederschulte H, Anderlei T, Knocke C, Klee D, Büchs J. 2006. Fed-batch mode in shake flasks by slow-release technique. Biotechnol Bioeng 95:433-445.
4. Muffler K, Ulber R. 2005. Downstream processing in marine biotechnology. Adv Biochem Engin/Biotechnol 97:63-103.
5. Ng LT, Guthrie JT, Yuan YJ. 2000. UV cured biocompatible membrane for biosensor application. Polym Int 49:1017-1020.
6. Rajagopalan KV. 1980. Sulfite oxidase. In: Coughlan M, ed. Molybdenum and molybdenum-containing enzymes. Oxford: Pergamon Press, p 242.
7. Rodin IV, Margulis EV. 1983. Solubility of magnesium, calcium and manganese sulfites in water. Russ J Inorg Chem 28:258-259.
8. Sasaki S, Arikawa Y, Shimomura M, Ikebukuro K, Karube I. 1997. Measurement of sulfite using sulfite oxidase and luminol chemiluminescence. Anal Commun 34:299-301.
9. Simon RA, Green L, Stevenson DD. 1982. The incidence of ingested metabisulfite sensitivity in an asthmatic population. J Allergy Clin Immunol 69:118-125.
10. Situmorang M, Hibbert DB, Gooding JJ, Barnett D. 1999. A sulfite biosensor fabricated using electrodeposited polytyramine: Application to wine analysis. Analyst 124:1775-1779.
11. Smith VJ. 1987. Determination of sulfite using a sulfite oxidase enzyme electrode. Anal Chem 59:2256-2259.
12. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC. 1985. Measurement of protein using bicinchoninic acid. Anal Biochem 150:76-85.
13. Sorokin DY. 1995. Sulfitobacter pontiacus gen. nov., sp. nov. - A new Heterotrophic Bacterium from the black sea, specialized of sulfite oxidation. Microbiology 64:295-305.
14. Stevenson DD, Simon RA. 1981. Sensitivity to ingested metabisulfites in asthmatic subjects. J Allergy Clin Immunol 68:26-32.
15. van't Riet K. 1979. Review of measuring methods and results in nonviscous gas-liquid mass transfer in stirred vessels. Ind Eng Chem Process Des Dev 18:357-364.