Structure determinations of human and chimaeric antibodies by solution scattering and constrained molecular modelling

Biochemical Society Transactions

Volumn 36, Issue 1, 2008, Pages 37-42

  Perkins, Stephen J ^a   Bonner, Alexandra ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Analytical ultracentrifugation; Chimaeric antibody; Constrained modelling; Hinge conformation; Neutron scattering; X ray scattering

Indexed keywords

CHIMERIC ANTIBODY; IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN G; SERUM ALBUMIN;

ARTICLE; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; DATA ANALYSIS; HUMAN; MACROMOLECULE; MOLECULAR BIOLOGY; MOLECULAR MODEL; NEUTRON SCATTERING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; RADIATION SCATTERING; SIMULATION; STRUCTURE ANALYSIS; ULTRACENTRIFUGATION;

ALGORITHMS; ANIMALS; ANTIBODIES; HUMANS; IMMUNOGLOBULIN A; MICE; MODELS, MOLECULAR; RECOMBINANT FUSION PROTEINS; SCATTERING, RADIATION; SOLUTIONS;

CHIMAERIFORMES;

EID: 39449131699     PISSN: 03005127     EISSN: None     Source Type: Journal    
DOI: 10.1042/BST0360037     Document Type: Article

References (19)

1. Glatter, O. and Kratky, O. (eds) (1982) Small Angle X-ray Scattering, Academic Press, New York
2. Perkins, S.J., Ashton, A.W., Boehm, M.K. and Chamberlain, D. (1998) Molecular structures from low angle X-ray and neutron scattering studies. Int. J. Biol. Macromol. 22, 1-16
3. Perkins, S.J., Okemefuna, A.I., Fernando, A.N., Bonner, A., Gilbert, H.E. and Furtado, P.B. (2008) X-ray and neutron scattering data and their constrained molecular modelling. Methods Cell Biol. 84, 375-423
4. Perkins, S.J. (2000) High-flux X-ray and neutron scattering studies, in Protein-Ligand Interactions: A Practical Approach (Chowdhry, B. and Harding, S.E., eds), pp. 223-262, Oxford University Press, Oxford
5. Perkins, S.J. and Furtado, P.B. (2005) Complement and immunoglobulin protein structures by X-ray and neutron solution scattering and analytical ultracentrifugation. in Structural Biology of the Complement System (Morikis, D. and Lambris, J.D., eds), pp. 293-315, Taylor and Francis, Boca Raton
6. Perkins, S.J., Nealis, A.S., Sutton, B.J. and Feinstein, A. (1991) The solution structure of human and mouse immunoglobulin IgM by synchrotron X-ray scattering and molecular graphics modelling: a possible mechanism for complement activation. J. Mol. Biol. 221, 1345-1366
7. Boehm, M.K., Woof, J.M., Kerr, M.A. and Perkins, S.J. (1999) The Fab and Fc fragments of IgA1 exhibit a different arrangement from that in IgG: a study by X-tay and neutron solution scattering and homology modelling. J. Mol. Biol. 286, 1421-1447
8. Perkins, S.J. (2001) X-ray and neutron scattering analyses of hydration shells: a molecular interpretation based on sequence predictions and modelling fits. Biophys. Chem. 93, 129-139
9. Ashton, A.W., Boehm, M.K., Gallimore, J.R., Pepys, M.B. and Perkins, S.J. (1997) Pentameric and decameric structures in solution of the serum amyloid P component by X-ray and neutron scattering and molecular modelling analyses. J. Mol. Biol. 272, 408-422
10. Furtado, P.B., Whitty, P.W., Robertson, A., Eaton, J.T., Almogren, A., Kerr, M.A., Woof, J.M. and Perkins, S.J. (2004) Solution structure determination of human IgA2 by X-ray and neutron scattering and analytical ultracentrifugation and constrained modelling: a comparison with human IgA1. J. Mol. Biol. 338, 921-941
11. Bonner, A., Perrier, C., Corthésy, B. and Perkins, S.J. (2007) Solution structure of human secretory component and implications for biological function. J. Biol. Chem. 282, 16969-16980
12. Garcia de la Torre, J., Huertas, M.L. and Carrasco, B. (2000) Calculation of hydrodynamic properties of globular proteins from their atomic-level structure. Biophys. J. 78, 719-730
13. Perkins, S.J., Gilbert, H.E., Lee, Y.C., Sun, Z. and Furtado, P.B. (2005) Relating small angle scattering and analytical ultracentrifugation in multidomain proteins, in Modern Analytical Ultracentrifugation: Techniques and Methods (Scott, D.J., Harding, S.E. and Rowe, A.J., eds), pp. 291-319, Royal Society of Chemistry, London
14. Sun, Z., Almogren, A., Furtado, P.B., Chowdhury, B., Kerr, M.A. and Perkins, S.J. (2005) Semi-extended solution structure of human myeloma immunoglobulin D determined by constrained X-ray scattering. J. Mol. Biol. 353, 155-173
15. Aslam, M., Guthridge, J.M., Hack, B.K., Quigg, R.J., Holers, V.M. and Perkins, S.J. (2003) The extended multidomain solution structures of the complement protein Crry and its chimaeric conjugate Crry-Ig by scattering, analytical ultracentrifugation and constrained modelling: implications for function and therapy. J. Mol. Biol. 329, 525-550
16. Gilbert, H.E., Aslam, M., Guthridge, J.M., Holers, V.M. and Perkins, S.J. (2006) Extended linkers between CR2 SCR-1 and SCR-2 and the Fc fragment in CR2-Ig by X-ray and neutron scattering, analytical ultracentrifugation and constrained molecular modelling. J. Mol. Biol. 356, 397-412
17. Boehm, M.K. and Perkins, S.J. (2000) Structural models for carcinoembryonic antigen and its complex with the single-chain Fv antibody molecule MFE23. FEBS Lett. 475, 11-16
18. Bonner, A., Furtado, P.B., Almogren, A., Kerr, M.A. and Perkins, S.J. (2008) Implications of the near-planar solution structure of human myeloma dimeric IgA1 for mucosal immunity and IgA nephropathy. J. Immunol. 180, in the press
19. Almogren, A., Furtado, P.B., Sun, Z., Perkins, S.J. and Kerr, M.A. (2006) Biochemical and structural properties of the complex between human immunoglobulin A1 and human serum albumin by X-ray and neutron scattering and analytical ultracentrifugation. J. Mol. Biol. 356, 413-431