Extended flexible linker structures in the complement chimaeric conjugate CR2-Ig by scattering, analytical ultracentrifugation and constrained modelling: Implications for function and therapy

Journal of Molecular Biology

Volumn 356, Issue 2, 2006, Pages 397-412

  Gilbert, Hannah E ^a   Aslam, Mohammed ^a   Guthridge, Joel M ^b   Holers, V Michael ^b   Perkins, Stephen J ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF COLORADO   (United States)

Author keywords

Analytical ultracentrifugation; Constrained modelling; Neutron scattering; Short consensus complement repeat; X ray scattering

Indexed keywords

COMPLEMENT COMPONENT C3D; COMPLEMENT COMPONENT C3D RECEPTOR; COMPLEMENT INHIBITOR; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G1;

AMINO ACID SEQUENCE; ARTICLE; CHIMERA; COMPLEMENT ACTIVATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; GLYCOSYLATION AND DEGLYCOSYLATION; HUMAN; HUMAN CELL; IMMUNOMODULATION; LIGAND BINDING; LIGHT CHAIN; MOLECULAR WEIGHT; MOUSE; NEUTRON SCATTERING; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; RADIATION SCATTERING; SEDIMENTATION RATE; STEREOCHEMISTRY; ULTRACENTRIFUGATION;

EID: 30744466395     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2005.11.050     Document Type: Article

References (53)

1. E. Fischer, C. Delibrias, and M.D. Kazatchkine Expression of CR2 (the C3d,g/EBV receptor, CD21) on normal human peripheral blood T lymphocytes J. Immunol. 146 1991 865 869
2. D. Watry, J.A. Hedrick, S. Siervo, G. Rhodes, J.J. Lamberti, J.D. Lambris, and C.D. Tsoukas Infection of human thymocytes by Epstein-Barr virus J. Expt. Med. 173 1991 971 980
3. M. Birkenbach, X. Tung, L.E. Bradbury, T.F. Tedder, and E. Kieff Characterization of an Epstein-Barr virus receptor on human epithelial cells J. Expt. Med. 176 1992 1405 1414
4. M. Reynes, J.P. Aubert, J.H.M. Cohen, J. Audouin, V. Tricottet, J. Diebold, and M.D. Kazatchkine Human follicular dendritic cells express CRI, CR2, and CR3 complement receptor antigens J. Immunol. 135 1985 2687 2694
5. G.B. Klaus, and J.H. Humphrey A re-evaluation of the role of C3 in B-cell activation Immunol. Today 7 1986 163 165
6. B. Heyman, E.J. Wiersma, and T. Kinoshita In vivo inhibition of the antibody response by a complement receptor-specific monoclonal antibody J. Expt. Med. 172 1990 665 668
7. G. Thyphronitis, T. Kinoshita, K. Inoue, J.E. Schweinle, G.S. Tsokos, and E.S. Metcalf Modulation of complement receptors I and 2 suppress antibody response in vivo J. Immunol. 147 1991 224 230
8. P. Björck, C. Elenstrom-Magnusson, A. Rosen, E. Severinson, and S. Paulie CD23 and CD21 function as adhesion molecules in homotypic aggregation of human B lymphocytes Eur. J. Immunol. 23 1993 1771 1775
9. E. Levy, J. Ambrus, L. Kahl, H. Molina, K. Tung, and V.M. Holers T lymphocyte expression of complement receptor 2 (CR2/CD21): a role in adhesive cell-cell interactions and dysregulation in a patient with systemic lupus erythematosus (SLE) Clin. Expt. lmmunol. 90 1992 235 244
10. J.-P. Aubry, S. Pochon, P. Graber, K.V. Jansen, and J.-Y. Bonnefoy CD21 is a ligand for CD23 and regulates lgE production Nature 358 1992 505 507
11. J.-P. Aubry, S. Pochon, J.-F. Gauchat, A. Nueda-Marin, V.M. Holers, and P. Graber CD23 interacts with a new functional extracytoplasmic domain involving N-linked oligosaccharides on CD21 J. Immunol. 152 1994 5806 5813
12. B. Nagar, R.G. Jones, R.J. Diefenbach, D.E. Isenman, and J.M. Rini X-ray crystal structure of C3d: a C3 fragment and ligand for complement receptor 2 Science 280 1998 1277 1281
13. G. Szakonyi, J.M. Guthridge, D. Li, K.A. Young, V.M. Holers, and X.S. Chen Structure of complement receptor 2 in complex with its C3d ligand Science 1725 2001 1728
14. A.E. Prota, D.R. Sage, T. Stehle, and J.D. Fingeroth The crystal structure of human CD21: Implications for Epstein-Barr virus and C3d binding Proc. Natl Acad. Sci. USA 99 2002 10641 10646
15. J.M. Guthridge, J.K. Rakstang, K.A. Young, J. Hinshelwood, M. Aslam, and A. Robertson Structural studies in solution of the recombinant N-terminal pair of short consensus/complement repeat domains of complement receptor type 2 (CR2/CD21) and its interaction with its ligand C3dg Biochemistry 40 2001 5931 5941
16. L. Clemenza, and D.E. Isenman Structure-guided identification of C3d residues essential for its binding to complement receptor 2 (CD21) J. Immunol. 165 2000 3839 3848
17. J.P. Hannan, K.A. Young, J.M. Guthridge, R. Asokan, G. Szakonyi, X.S. Chen, and V.M. Holers Mutational analysis of the complement receptor type 2 (CR2/CD21)-C3d interaction reveals a putative charged SCR 1 binding site for C3d J. Mol. Biol. 346 2005 845 858
18. H.E. Gilbert, J.T. Eaton, J.P. Hannan, V.M. Holers, and S.J. Perkins Solution structure of the complex between CR2 SCR 1-2 and C3d of human complement: an X-ray scattering and sedimentation modelling study J. Mol. Biol. 346 2005 859 873
19. M.D. Moore, R.G. DiScipio, N.R. Cooper, and G.R. Nemerow Hydrodynamic, electron microscopic and ligand-binding analysis of the Epstein-Barr virus/C3dg receptor (CR2) J. Biol. Chem. 264 1989 20576 20582
20. S.J. Perkins, A.W. Ashton, M.K. Boehm, and D. Chamberlain Molecular structures from low angle X-ray and neutron scattering strudies Int. J. Biol. Macromol. 22 1998 1 16
21. M. Aslam, J.M. Guthridge, B.K. Hack, R.J. Quigg, V.M. Holers, and S.J. Perkins The extended multidomain solution structures of the complement protein Crry and its chimaeric conjugate Crry-Ig by scattering, analytical ultracentrifugation and constrained modelling: implications for function and therapy J. Mol. Biol. 329 2003 525 550
22. T. Hebell, J.M. Ahearn, and D.T. Fearon Suppression of the immune response by a soluble complement receptor of B lymphocytes Science 254 1991 102 105
23. H. Song, C. He, C. Knaak, J.M. Guthridge, V.M. Holers, and S. Tomlinson Complement receptor 2-mediated targeting of complement inhibitors to sites of complement activation J Clin Invest. 111 2003 1875 1885
24. S.J. Perkins X-ray and neutron solution scattering New Compr. Biochem. 11B 1988 143 265
25. M. Aslam, and S.J. Perkins Folded-back solution structure of monomeric Factor H of human complement by synchrotron X-ray and neutron scattering, analytical ultracentrifugation and constrained molecular modelling J. Mol. Biol. 309 2001 1117 1138
26. M.K. Boehm, J.M. Woof, M.A. Kerr, and S.J. Perkins The Fab and Fc fragments of IgA1 exhibit a different arrangement from that in IgG: a study by X-ray and neutron solution scattering and homology modelling J. Mol. Biol. 286 1999 1421 1447
27. P.B. Furtado, P.W. Whitty, A. Robertson, J.T. Eaton, A. Almogren, and M.A. Kerr Solution structure determination of monomeric human IgA2 by X-ray and neutron scattering, analytical ultracentrifugation and constrained modelling: a comparison with monomeric human IgA1 J. Mol. Biol. 338 2004 921 941
28. Z. Sun, A. Almogren, P.B. Furtado, B. Chowdhury, M.A. Kerr, and S.J. Perkins Semi-extended solution structure of human myeloma immunoglobulin D determined by constrained X-ray scattering J. Mol. Biol. 353 2005 155 173
29. S.J. Perkins X-ray and neutron scattering analyses of hydration shells: a molecular interpretation based on sequence predictions and modelling fits Biophys. Chem. 93 2001 129 139
30. L.J. Harris, E. Skaletsky, and A. McPherson Crystallographic structure of an intact IgG1 monoclonal antibody J. Mol. Biol. 275 1998 861 872
31. M.D. Moore, M.J. Cannon, A. Sewall, M. Finlayson, M. Okimoto, and G.R. Nemerow Inhibition of Epstein-Barr virus infection in vitro and in vivo by soluble CR2 (CD21) containing two short consensus repeats J. Virol. 65 1991 3559 3565
32. R.J. Quigg, Y. Kozono, D. Berthiaume, A. Lim, D.J. Salant, and A. Weinfeld Blockade of antibody-induced glomerulonephritis with Crry-Ig, a soluble murine complement inhibitor J. Immunol. 160 1998 4553 4560
33. M. Basta, and C.H. Hammer A rapid FPLC method for purification of the third component of human and guinea pig complement J. Immunol. Methods 142 1991 39 44
34. K.J. Micklem, R.B. Sim, and E. Sim Analysis of C3-receptor activity on human B-lymphocytes and isolation of the complement receptor type 2 (CR2) Biochem. J. 224 1984 75 86
35. S.J. Perkins Protein volumes and hydration effects: the calculation of partial specific volumes, neutron scattering matchpoints and 280 nm absorption coefficients for proteins and glycoproteins from amino acid sequences Eur. J. Biochem. 157 1986 169 180
36. E. Towns-Andrews, A. Berry, J. Bordas, G.R. Mant, P.K. Murray, and K. Roberts Time-resolved X-ray diffraction station: X-ray optics, detectors and data acquisition Rev. Sci. Instrum. 60 1989 2346 2349
37. J.S. Worgan, R. Lewis, N.S. Fore, I.L. Sumner, A. Berry, and B. Parker The application of multiwire X-ray detectors to experiments using synchrotron radiation Nucl. Instrum. Methods Phys. Res. A 291 1990 447 454
38. C. Boulin, R. Kempf, M.H.J. Koch, and S.M. McLaughlin Data appraisal, evaluation and display for synchrotron radiation experiments: hardware and software Nucl. Instrum. Meth. A249 1986 399 407
39. P. Lindner, R.P. May, and P.A. Timmins Upgrading of the SANS instrument D11 at the ILL Phys. B 180 1992 967 972
40. Ghosh, R. E. (1989). A Computing Guide for Small Angle Scattering Experiments. ILL Internal publication 89GH02T.
41. Heenan, R. K. & King, S. M. (1993). Development of the small-angle diffractometer LOQ at the ISIS pulsed neutron source. In Proceedings of an International Seminar on Structural Investigations at Pulsed Neutron Sources, Dubna, 1st-4th September 1992. Report E3-93-65, Joint Institute for Nuclear Research, Dubna.
42. G.D. Wignall, and F.S. Bates Absolute calibration of small angle neutron scattering data J. Appl. Crystallog. 20 1987 28 40
43. Heenan, R. K., King, S. M., Osborn, R. & Stanley, H. B. (1989). COLETTE Users Guide. Internal publication RAL-89-128, Rutherford Appleton Laboratory, Didcot, UK.
44. O. Glatter, and O. Kratky Small-angle X-ray Scattering 1982 Academic Press New York
45. O. Kratky X-ray small angle scattering with substances of biological interest in diluted solutions Progr. Biophys. Chem. 13 1963 105 173
46. A.V. Semenyuk, and D.I. Svergun GNOM-a program package for small-angle scattering data-processing J. Appl. Crystallog. 24 1991 537 540
47. D.I. Svergun Determination of the regularization parameter in indirect transform methods using perceptual criteria J. Appl. Crystallog. 25 1992 495 503
48. J. Philo A method for directly fitting the time derivative of sedimentation velocity data and an alternative algorithm for calculating sedimentation coefficient distribution functions Anal. Biochem. 279 2000 151 163
49. A.W. Ashton, M.K. Boehm, J.R. Gallimore, M.B. Pepys, and S.J. Perkins Pentameric and decameric structures in solution of the serum amyloid P component by X-ray and neutron scattering and molecular modelling analyses J. Mol. Biol. 272 1997 408 422
50. S.J. Perkins, and H. Weiss Low resolution structural studies of mitochondrial ubiquinol-cytochrome c reductase in detergent solutions by neutron scattering J. Mol. Biol. 168 1983 847 866
51. A.J. Beavil, R.J. Young, B.J. Sutton, and S.J. Perkins Bent domain structure of recombinant human IgE-Fc in solution by X-ray and neutron scattering in conjunction with an automated curve fitting procedure Biochemistry 34 1995 14449 14461
52. J. Garcia de la Torre, S. Navarro, M.C. Lopez Martinez, F.G. Diaz, and J. Lopez Cascales HYDRO. A computer software for the prediction of hydrodynamic properties of macromolecules Biophys. J. 67 1994 530 531
53. J. Garcia de la Torre, M.L. Huertas, and B. Carrasco Calculation of hydrodynamic properties of globular proteins from their atomic-level structure Biophys. J. 78 2000 719 730