Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity

Letters in Applied Microbiology

Volumn 46, Issue 3, 2008, Pages 371-376

  Oliveira, M D L ^a   Andrade, C A S ^a   Santos Magalhaes N S ^a   Coelho, L C B B ^a   Teixeira, J A ^b   Carneiro Da Cunha, M G ^a   Correia, M T S ^a  

^a FEDERAL UNIVERSITY OF PERNAMBUCO   (Brazil)

^b UNIVERSITY OF MINHO   (Portugal)

Author keywords

Antibacterial lectin; Bacteria; Eugenia uniflora; Eugenia uniflora seed; Lectin; Lectin purification

Indexed keywords

BACTERIOLOGY; BLOOD; CHROMATOGRAPHIC ANALYSIS; ELECTROPHORESIS; ESCHERICHIA COLI; ION CHROMATOGRAPHY; MICROWAVE INTEGRATED CIRCUITS; PROTEINS; PURIFICATION;

ANTI-BACTERIAL ACTIVITY; ANTI-MICROBIAL PROPERTIES; ANTIBACTERIAL LECTIN; ANTIBACTERIALS; EUGENIA UNIFLORA; EUGENIUM UNIFLORA SEED; LECTIN; LECTIN PURIFICATIONS; MINIMUM INHIBITORY CONCENTRATION;

ION EXCHANGE;

EUGENIA UNIFLORA EXTRACT; LECTIN;

BACTERIA; CARBOHYDRATES; DENATURATION; ELECTROPHORESIS; ION EXCHANGE; MOLECULAR MASS; PH EFFECTS; SEED;

ANTIMICROBIAL ACTIVITY; BACTERIUM; CARBOHYDRATE; ELECTROKINESIS; FRUIT; ION CHROMATOGRAPHY; ION EXCHANGE; PROTEIN; SEED; SMALL MAMMAL;

ANTIBACTERIAL ACTIVITY; ARTICLE; BACILLUS SUBTILIS; BACTERIAL GROWTH; DENATURATION; ELECTROPHORESIS; ESCHERICHIA COLI; GROWTH INHIBITION; HEAT TOLERANCE; HEMAGGLUTINATION; ION EXCHANGE CHROMATOGRAPHY; KLEBSIELLA; MICROBIOLOGY; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR WEIGHT; PROTEIN PURIFICATION; PSEUDOMONAS AERUGINOSA; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS;

BACILLUS SUBTILIS; ESCHERICHIA COLI; EUGENIA UNIFLORA; KLEBSIELLA SP.; ORYCTOLAGUS CUNICULUS; PSEUDOMONAS AERUGINOSA; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS SP.;

EID: 39149132154     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2007.02319.x     Document Type: Article

References (24)

1. Arai, I., Amagaya, S., Komatsu, Y., Okada, M., Hayashi, T., Kasai, M., Arisawa, M. Momose, Y. (1999) Improving effects of the extracts from Eugenia uniflora on hyperglycemia and hypertriglyceridemia in mice. J Ethnopharmacol 68, 307 314.
2. Ashford, D., Allen, K. Neuberger, A. (1982) The production and properties of an antiserum to potato (Solamum tuberosum) lectin. Biochem J 201, 641 645.
3. Basile, A., Vuotto, M.L., Violante, U., Sorbo, S., Martone, G. Castaldo-Cobianchi, R. (1997) Antibacterial activity in Actinidia chinensis, Feijoa sellowiana and Aberia caffra. Int J Antimicrob Agents 8, 199 203.
4. Calderon, A.M., Buck, G. Doyle, R.J. (1997) Lectin-microrganism complexes. In Lectins, Biology, Biochemistry, Clinical Biochemistry Vol. 12 ed. van Driessche, E., Beeckmans, S. Bøg-Hansen, T.C. Lemchesvej. Hellerup, Denmark: TEXTOP. http://plab.ku.dk/tcbh/Lectins12/Calderon/paper.htm.
5. Cole, M.D. (1994) Key antifungal, antibacterial and anti-insect assays - a critical review. Biochem Syst Ecol 22, 837 856.
6. Consolini, A.E., Baldini, O.A.N. Amat, A.G. (1999) Pharmacological basis for the empirical use of Eugenia uniflora L. (Myrtaceae) as antihypertensive. J Ethnopharmacol 66, 33 39.
7. Correia, M.T.S. Coelho, L.C.B.B. (1995) Purification of a glucose/mannose specific lectin, isoform 1, from seeds of Cratylia mollis Mart. (Camaratu bean). Appl Biochem Biotechnol 55, 261 273.
8. Courvalin, P., Goldstein, F., Philippon, A. Sirot, J. (1985) L'antibiogramme. Paris, Bruxelles : MPC-Videocom.
9. Doyle, R.J. (1994) Introduction to lectins and their interactions with microorganisms. In Lectin-microorganism Interactions ed. Doyle, R.J. Slifkin, M.V. pp. 1 65. New York : Marcel Dekker, Inc.
10. Goldstein, I.J. Poretz, R.D. (1986) Isolation, physicochemical characterization and carbohydrate-binding specificity of lectins. In The Lectins: Properties, Functions and Applications in Biology and Medicine ed. Liener, I.E., Sharon, N. Goldstein, I.J.V. pp. 33 247. Orlando, FL, USA : Academic Press.
11. Guzmán-Partida, A.M., Robles-Burgueño, M.R., Ortega-Nieblas, M. Vázquez-Moreno, I. (2004) Purification and characterization of complex carbohydrate specific isolectins from wild legume seeds: Acacia constricta is (vinorama) highly homologous to Phaseolus vulgaris lectins. Biochimie 86, 335 342.
12. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680 685.
13. Latha, V.L., Rao, R.N. Nadimpalli, S.K. (2006) Affinity purification, physicochemical and immunological characterization of a galactose-specific lectin from the seeds of Dolichos lablab (Indian lablab beans). Protein Expr Purif 45, 296 306.
14. Lee, M.H., Chiou, J.F., Yen, K.Y. Yang, L.L. (2000) EBV DNA polymerase inhibition of tannins from Eugenia uniflora. Cancer Lett 154, 131 136.
15. Lima, V.L.M., Correia, M.T.S., Cechinel, Y.M.N., Sampaio, C.A., Ownen, J.S. Coelho, L.C.B.B. (1997) Immobilized Cratylia mollis lectin as a potential matrix to isolate plasma glycoproteins including lecithin-cholesterol acyltransferase. Carbohydr Polym 33, 27 32.
16. Munoz-Crego, A., Alvarez, O., Alonso, B., Rogers, D.J. Lvovo, J. (1999). Lectin as diagnostic probes in clinical bacteriology-an overview. In Lectins, Biology, Biochemistry, Clinical Biochemistry Vol. 13 ed. van Driessche, E., Beeckmans, S. Bøg-Hansen, T.C. Lemchesvej. Hellerup, Denmark: TEXTOP. http://plab.ku.dk/tcbh/Lectins12/Calderon/paper.htm.
17. Ngai, P.H.K. Ng, T.B. (2004) A mushroom (Ganoderma capense) lectin with spectacular thermostability, potent mitogenic activity toward tumor cells. Biochem Biophys Res Commun 314, 988 993.
18. Ratanapo, S., Ngamjunyaporn, W. Chulavatnatol, M. (2001) Interaction of a mulberry leaf lectin with a phytopathogenic bacterium, P. syringae pv mori. Plant Sci 160, 739 744.
19. Sharon, N. Lis, H. (1990) Legum lectins, a large family of homologous proteins. FASEB J 4, 3198 3208.
20. Sharon, N. Lis, H. (1995) Lectins with a sweet tooth: functions in cell recognition. Essays Biochem 30, 59 75.
21. Slifkin, M. Doyle, R.J. (1990) Lectins and their application in clinical microbiology. Clin Microbiol Rev 3, 197 218.
22. Talas-Ogras, T., Ipekci, Z., Bajrovic, K. Gozukirmizi, N. (2005) Antibacterial activity of seed proteins of Robinia pseudoacacia. Fitoterapia 76, 67 72.
23. Wong, J.H., Wong, C.C.T. Ng, T.B. (2006) Purification and characterization of a galactose-specific lectin with mitogenic activity from pinto beans. Biochim Biophys Acta 1760, 808 813.
24. Zeng, X., Nakaaki, Y., Murata, T. Usui, T. (2000) Chemoenzymatic synthesis of glycopeptides carrying α-Neu5Ac-(2→3)-α-D-GalNAc, and related compounds and analysis of their specific interactions with lectins. Arch Biochem Biophys 383, 28 37.