Construction of expression vector of HTERT- HIL18 fusion gene and induction of cytotoxic T lymphocyte response against hTERT

European Journal of Medical Research

Volumn 13, Issue 1, 2008, Pages 7-14

  Tong, X M ^a   Zheng, S E ^a   Bader, A ^b   Yao, H P ^a   Wu, N P ^a   Altmeyer, P ^b   Brockmeyer, N H ^b   Jin, Jie ^a  

^a ZHEJIANG UNIVERSITY SCHOOL OF MEDICINE   (China)

^b RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

Dendritic cells; Fusion gene; Interleukin 18; Telomerase reverse transcriptase

Indexed keywords

DENDRITIC CELL VACCINE; HYBRID PROTEIN; INTERLEUKIN 18; INTERLEUKIN 18 TELOMERASE REVERSE TRANSCRIPTASE FUSION PROTEIN; TELOMERASE REVERSE TRANSCRIPTASE; TUMOR VACCINE; UNCLASSIFIED DRUG;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL STRAIN 3T3; CELL TRANSFER; CONTROLLED STUDY; CYTOTOXIC T LYMPHOCYTE; DENDRITIC CELL; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSION VECTOR; FUSION GENE; GENE INSERTION; GENE SEQUENCE; GENE TRANSFER; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNE RESPONSE; IMMUNOFLUORESCENCE; IMMUNOSTIMULATION; NONHUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; T LYMPHOCYTE ACTIVATION; WESTERN BLOTTING;

3T3 CELLS; ANIMALS; APOPTOSIS; CANCER VACCINES; CELL LINE, TUMOR; COCULTURE TECHNIQUES; CULTURE MEDIA, CONDITIONED; CYTOTOXICITY TESTS, IMMUNOLOGIC; DENDRITIC CELLS; GENETIC VECTORS; HUMANS; INTERFERON TYPE II; INTERLEUKIN-18; K562 CELLS; LEUKOCYTES, MONONUCLEAR; METHOTREXATE; MICE; RECOMBINANT FUSION PROTEINS; T-LYMPHOCYTES, CYTOTOXIC; TELOMERASE; TRANSFECTION;

EID: 38949185310     PISSN: 09492321     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Vonderheide RH. Telomerase as a universal tumor-associated antigen for cancer immunotherapy. Oncogene. 2002 Jan 21;21(4):674-9
2. Cao Y, Li H, Deb S, Liu JP. TERT regulates cell survival independent of telomerase enzymatic activity. Oncogene. 2002 May 9;21(20):3130-8
3. Hess PR, Boczkowski D, Nair SK, Snyder D, Gilboa. Vaccination with mRNAs encoding tumor-associated antigens and granulocyte-macrophage colony-stimulating factor efficiently primes CTL responses, but is insufficient to overcome tolerance to a model tumor/self antigen. Cancer Immunol Immunother. 2006 Jun;55(6):672-83.
4. Sheng KC, Pietersz GA, Wright MD, Apostolopoulos V. Dendritic cells: activation and maturation - applications for cancer immunotherapy. Curr Med Chem. 2005;12(15): 1783-800.
5. Heiser A, Maurice MA, Yancey DR, Coleman DM, Dahm P, Vieweg J.Human dendritic cells transfected with renal tumor RNA stimulate polyclonal T-cell responses against antigens expressed by primary and metastatic tumors. Cancer Res. 2001 Apr 15;61(8):3388-93
6. Hashimoto W, Tanaka F, Robbins PD, Taniguchi M, Okamura H, Lotze MT, Tahara H. Natural killer, but not natural killer T, cells play a necessary role in the promotion of an innate antitumor response induced by IL-18. Int J Cancer. 2003 Feb 10;103(4):508-13.
7. Tatsumi T, Gambotto A, Robbins PD, Storkus WJ. Interleukin 18 gene transfer expands the repertoire of antitumor Th1-type immunity elicited by dendritic cell-based vaccines in association with enhanced therapeutic efficacy. Cancer Res. 2002 Oct 15;62(20):5853-8
8. Vonderheide RH, Domchek SM, Schultze JL, et al. Vaccination of cancer patients against telomerase induces functional antitumor CD8+ T lymphocytes. Clin Cancer Res. 2004 Feb 1;10(3):828-39.
9. Kedde M, le Sage C, Duursma A,Telomerase-independent regulation of ATR by human telomerase RNA. J Biol Chem. 2006 281 (52):40503-14
10. Iczkowski KA, Pantazis CG, McGregor DH, Wu Y, Tawfik OW. Telomerase reverse transcriptase subunit immunoreactivity: a marker for high-grade prostate carcinoma. Cancer. 2002 Dec 15;95(12):2487-93
11. Saeboe-Larssen S, Fossberg E, Gaudernack G Characterization of novel alternative splicing sites in human telomerase reverse transcriptase (hTERT): analysis of expression and mutual correlation in mRNA isoforms from normal and tumour tissues. BMC Mol Biol. 2006 Aug 29;7:26
12. Carpenter EL, Vonderheide RH telomerase-based immunotherapy of cancer. Expert Opin Biol Ther. 2006 Oct; 6(10):1031-9
13. Nair SK, Heiser A, Boczkowski D, Majumdar A, Naoe M, Lebkowski JS, Vieweg J, Gilboa E. Induction of cytotoxic T cell responses and tumor immunity against unrelated tumors using telomerase reverse transcriptase RNA transfected dendritic cells. Nat Med. 2000 Sep;6(9):1011-7.
14. Liebau C, Merk H, Roesel C, Schmidt S, Karreman C, Prisack JB, Bojar H, Baltzer AW rIL-18 triggered gene therapy based on a transduction with the IL-12 plasmid: a new option as immuno-therapy for osteosarcoma. Anti-cancer Res. 2002 Sep;22(5):2559-65
15. Yamanaka R, Yajima N, Tsuchiya N, Honma J, Tanaka R, Ramsey J, Blaese M, Xanthopoulos KG. Administration of interleukin-12 and -18 enhancing the antitumor immunity of genetically modified dendritic cells that had been pulsed with Semliki forest virus-mediated tumor complementary DNA. J Neurosurg. 2002 Nov;97(5):1184-90
16. Xia D, Li F, Xiang J. Engineered fusion hybrid vaccine of IL-18 gene-modified tumor cells and dendritic cells induces enhanced antitumor immunity. Cancer Biother Radiopharm. 2004 Jun;19(3):322-30
17. Yamanaka R, Honma J, Tsuchiya N, Yajima N, Kobayashi T, Tanaka R. Tumor lysate and IL-18 loaded dendritic cells elicits Th1 response, tumor-specific CD8+ cytotoxic T cells in patients with malignant glioma. J Neurooncol. 2005 Apr;72(2):107-13.
18. Yamanaka R, Xanthopoulos KG. Induction of antigen-specific immune responses against malignant brain tumors by intramuscular injection of sindbis DNA encoding gp100 and IL-18. DNA Cell Biol. 2005 May;24(5): 317-24
19. Culshaw S, Leung BP, Gracie JA, Campbell CC, Thomson D, Gemmell C, Liew FY, McInnes IB.Prior elevation of IL-18 promotes rapid early IFN-gamma production during staphylococcal infection. Eur J Immunol. 2005 May;35(5):1438-44
20. Zhang P, Chan SL, Fu W, Mendoza M, Mattson MP. TERT suppresses apoptotis at a premitochondrial step by a mechanism requiring reverse transcriptase activity and 14-3-3 protein-binding ability. FASEB J. 2003 Apr;17(6): 767-9.
21. Xia D, Zheng S, Zhang W, He L, Wang Q, Pan J, Zhang L, Wang J, Cao X. Effective induction of therapeutic anti-tumor immunity by dendritic cells coexpressing interleukin-18 and tumor antigen. J Mol Med. 2003 Sep; 81(9): 585-96.
22. Lu JX, Peng Y, Meng ZF, Jin LQ, Lu YS, Guan MX. Rational design of an EGF-IL18 fusion protein: implication for developing tumor therapeutics. Biochem Biophys Res Commun. 2005 Aug 19; 334(1):157-61
23. Ghosh U, Bhattacharyya NP Benzamide and 4-amino 1,8 naphthalimide treatment inhibit telomerase activity by down-regulating the expression of telomerase associated protein and inhibiting the poly(ADP-ribosyl)ation of telomerase reverse transcriptase in cultured cells. FEBS J. 2005 Aug;272(16):4237-48.
24. Culshaw S, Leung BP, Gracie JA. Prior elevation of IL-18 promotes rapid early IFN-gamma production during staphylococcal infection. Eur J Immunol. 2005 May;35(5): 1438-44