Astrocytes shed extracellular vesicles that contain fibroblast growth factor-2 and vascular endothelial growth factor

International Journal of Molecular Medicine

Volumn 21, Issue 1, 2008, Pages 63-67

  Proia, Patrizia ^a   Schiera, Gabriella ^a   Mineo, Marco ^a   Ingrassia, Antonia Maria Rita ^a   Santoro, Graziella ^a   Savettieri, Giovanni ^a   Di Liegro, Italia ^a  

^a UNIVERSITY OF PALERMO   (Italy)

Author keywords

Astrocytes; Extracellular vesicle shedding; Fibroblast growth factor 2; Vascular endothelial growth factor

Indexed keywords

ANGIOGENIC FACTOR; BETA1 INTEGRIN; FIBROBLAST GROWTH FACTOR 2; VASCULOTROPIN;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; ASTROCYTE; CELL VACUOLE; CONTROLLED STUDY; CULTURE MEDIUM; IMMUNOFLUORESCENCE; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; RAT; SCANNING ELECTRON MICROSCOPY; WESTERN BLOTTING;

EID: 38949152658     PISSN: 11073756     EISSN: None     Source Type: Journal    
DOI: 10.3892/ijmm.21.1.63     Document Type: Article

References (22)

1. Schiera G, Bono E, Raffa MP, Gallo A, Pitarresi GL, Di Liegro I and Savettieri G: Synergistic effects of neurons and astrocytes on the differentiation of brain capillary endothelial cells in culture. J Cell Mol Med 7: 165-170, 2003.
2. Schiera G, Sala S, Gallo A, Raffa MP, Pitarresi GL, Savettieri G and Di Liegro I: Permeability properties of a three-cell type in vitro model of blood-brain barrier. J Cell Mol Med 9: 373-379, 2005.
3. Schiera G and Proia P: In vitro models of blood-brain barrier formation and functioning. In: Molecular Bases of Neuro-degeneration. Di Liegro I and Savettieri G (eds). Research Signpost, Kerala, pp 183-197, 2005.
4. Nakagawa T and Schwartz JP: Gene expression patterns in vivo normal adult astrocytes compared with cultured neonatal and normal adult astrocytes. Neurochem Int 45: 203-242, 2004.
5. Taverna S, Ghersi G, Ginestra A, Rigoglioso S, Pecorella S, Alaimo G, Saladino F, Dolo V, Dell'Era P, Pavan A, Pizzolanti G, Mignatti P, Presta M and Vittorelli ML: Shedding of membrane vesicles mediates fibroblast growth factor-2 release from cells. J Biol Chem 278: 51911-51919, 2003.
6. Basilico C and Moscatelli D: The FGF family of growth factors and oncogenes. Adv Cancer Res 59: 115-165, 1992.
7. Bikfalvi A, Klein S, Pintucci G and Rifkin DB: Biological roles of fibroblast growth factor-2. Endocr Rev 18: 26-45, 1997.
8. Taraboletti G, D'Ascenzo S, Giusti I, Marchetti D, Borsotti P, Millimaggi D, Giavazzi R, Pavan A and Dolo V: Bioavailability of VEGF in tumor-shed vesicles depends on vesicle burst induced by acidic pH. Neoplasia 8: 96-103, 2006.
9. Ferrara N and Davis-Smith T: The biology of vascular endothelial growth factor. Endocr Rev 18: 4-25, 1997.
10. Huez I, Bornes S, Bresson D, Créancier L and Prats H: New vascular endothelial isoform generated by internal ribosomal entry site-driven CUG translation initiation. Mol Endocrinol 15: 2197-2210, 2001.
11. Tee MK and Jaffe RB: A precursor form of vascular endothelial growth factor arises by initiation from an upstream in-frame CUG codon. Biochem J 359:219-226,2001.
12. D'Agostino S, Salomone M, Di Liegro I and Vittorelli ML: Membrane vesicles shed by oligodendrogliorna cells induce neuronal apoptosis. Int J Oncol 29: 1075-1085, 2006.
13. Schiera G, Proia P, Alberti C, Mineo M, Savettieri G and Di Liegro I: Neurons produce FGF2 and VEGF and secrete them at least in part by shedding extracellular vesicles. J Cell Mot Med (In press).
14. Dolo V, Ginestra A, Cassara D, Violini S, Lucania G, Torrisi MR, Nagase H, Canevari S, Pavan A and Vittorelli ML: Selective localization of matrix metalloproteinase 9, beta1 integrins, and human lymphocyte antigen class I molecules on membrane vesicles shed by 8701-BC breast carcinoma cells. Cancer Res 58: 4468-4474, 1998.
15. Cole R and de Vellis J: Preparation of astrocytes and oligodendrocyte cultures from primary rat glial cultures. In: A Dissection and Tissue Culture Manual of the Nervous System. Alan R Liss Inc, New York, pp 121-133, 1989.
16. Cestelli A, Savettieri G, Ferraro D and Vitale F: Formulation of a novel synthetic medium for selectively culturing rat CNS neurons. Dev Brain Res 22: 219-227, 1985.
17. Dolo V, Ginestra A, Ghersi G, Nagase H and Vittorelli ML: Human breast carcinoma cells cultured in the presence of serum shed membrane vesicles rich in gelatinolytic activities. J Submicrosc Cytol Pathol 26: 173-180, 1994.
18. Savettieri G, Di Liegro I, Catania C, Licata L, Pitarresi GL, D'Agostino S, Schiera G, De Caro V, Giandalia G, Giannola LI and Cestelli A: Neurons and ECM regulate occludin localization in brain endothelial cells. Neuroreport 11: 1081-1084, 2000.
19. Cestelli A, Catania C, D'Agostino S, Di Liegro I, Licata L, Schiera G, Pitarresi GL, Savettieri G, De Caro V, Giandalia G and Giannola LI: Functional feature of a novel model of blood brain barrier: studies on permeation of test compounds. J Control Release 76: 139-147, 2001.
20. Vittorelli ML: Shed membrane vesicles and clustering of membrane-bound proteolytic enzymes. Curr Top Dev Biol 54: 411-432, 2003.
21. Pilzer D, Gasser O, Moskovich O, Schifferli JA and Fishelson Z: Emission of membrane vesicles: roles in complement resistance, immunity and cancer. Springer Semin Immunopathol 27: 375-387, 2005.
22. Whiteside TL: Tumour-derived exosomes or microvesicles: another mechanism of tumour escape from the host immune system? Br J Cancer 92: 209-211, 2005.