Generation and application of new rat monoclonal antibodies against synthetic FLAG and OLLAS tags for improved immunodetection

Journal of Immunological Methods

Volumn 331, Issue 1-2, 2008, Pages 27-38

  Park, Sung Ho ^a   Cheong, Cheolho ^a   Idoyaga, Juliana ^a   Kim, Jae Y ^a   Choi, Jae Hoon ^a   Do, Yoonkyung ^a   Lee, Haekyung ^a   Jo, Jung Heon ^a   Oh, Yong Seok ^a   Im, Wonpil ^b   Steinman, Ralph M ^a   Park, Chae Gyu ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b UNIVERSITY OF KANSAS   (United States)

Author keywords

FLAG tag; Monoclonal antibody; OLLAS tag; Protein tagging

Indexed keywords

EPITOPE; IMMUNOGLOBULIN G; LANGERIN; MONOCLONAL ANTIBODY; OUTER MEMBRANE PROTEIN F;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CARBOXY TERMINAL SEQUENCE; FLOW CYTOMETRY; IMMUNODETECTION; IMMUNOHISTOCHEMISTRY; IMMUNOPRECIPITATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DETERMINATION; RAT; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGENS, SURFACE; CELL LINE; EPITOPES; GENETIC VECTORS; IMMUNOASSAY; LECTINS, C-TYPE; MANNOSE-BINDING LECTINS; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; PEPTIDES; PORINS; RATS; RATS, WISTAR; RECOMBINANT FUSION PROTEINS; SENSITIVITY AND SPECIFICITY;

RATTUS;

EID: 38949087918     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2007.10.012     Document Type: Article

References (16)

1. Arai R., Ueda H., Kitayama A., Kamiya N., and Nagamune T. Design of the linkers which effectively separate domains of a bifunctional fusion protein. Protein Eng. 14 (2001) 529
2. Bryson K., McGuffin L.J., Marsden R.L., Ward J.J., Sodhi J.S., and Jones D.T. Protein structure prediction servers at University College London. Nucleic Acids Res. 33 (2005) W36 (Web Server issue)
3. Cheong C., Idoyaga J., Do Y., Pack M., Park S.H., Lee H., Kang Y.S., Choi J.H., Kim J.Y., Bonito A., Inaba K., Yamazaki S., Steinman R.M., and Park C.G. Production of monoclonal antibodies that recognize the extracellular domain of mouse Langerin/CD207. J. Immunol. Methods 324 (2007) 48
4. Evan G.I., Lewis G.K., Ramsay G., and Bishop J.M. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5 (1985) 3610
5. Galustian C., Park C.G., Chai W., Kiso M., Bruening S.A., Kang Y.S., Steinman R.M., and Feizi T. High and low affinity carbohydrate ligands revealed for murine SIGN-R1 by carbohydrate array and cell binding approaches, and differing specificities for SIGN-R3 and langerin. Int. Immunol. 16 (2004) 853
6. Hawiger D., Inaba K., Dorsett Y., Guo M., Mahnke K., Rivera M., Ravetch J.V., Steinman R.M., and Nussenzweig M.C. Dendritic cells induce peripheral T cell unresponsiveness under steady state conditions in vivo. J. Exp. Med. 194 (2001) 769
7. Hopp T.P., Prickett K.S., Price V., Libby R.T., March C.J., Cerretti P., Urdal D.L., and Conlon P.J. Synthesis of protein with an identification peptide (proteins). Bio/Technology (N.Y. N.Y., 1983) 6 (1988) 1205
8. Im W., and Roux B. Ions and counterions in a biological channel: a molecular dynamics simulation of OmpF porin from Escherichia coli in an explicit membrane with 1 M KCl aqueous salt solution. J. Mol. Biol. 319 (2002) 1177
9. Jarvik J.W., and Telmer C.A. Epitope tagging. Annu. Rev. Genet. 32 (1998) 601
10. Kang Y.-S., Yamazaki S., Iyoda T., Pack M., Bruening S., Kim J.Y., Takahara K., Inaba K., Steinman R.M., and Park C.G. SIGN-R1, a novel C-type lectin expressed by marginal zone macrophages in spleen, mediates uptake of the polysaccharide dextran. Int. Immunol. 15 (2003) 177
11. Kang Y.-S., Kim J.Y., Bruening S.A., Pack M., Charalambous A., Pritsker A., Moran T.M., Loeffler J.M., Steinman R.M., and Park C.G. The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen. Proc. Natl. Acad. Sci. U. S. A. 101 (2004) 215
12. Lindner P., Bauer K., Krebber A., Nieba L., Kremmer E., Krebber C., Honegger A., Klinger B., Mocikat R., and Pluckthun A. Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. Biotechniques 22 (1997) 140
13. McGuffin L.J., Bryson K., and Jones D.T. The PSIPRED protein structure prediction server. Bioinformatics 16 (2000) 404
14. Nygren P.A., Stahl S., and Uhlen M. Engineering proteins to facilitate bioprocessing. Trends Biotechnol. 12 (1994) 184
15. Southern J.A., Young D.F., Heaney F., Baumgartner W.K., and Randall R.E. Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J. Gen. Virol. 72 (1991) 1551
16. Wilson I.A., Niman H.L., Houghten R.A., Cherenson A.R., Connolly M.L., and Lerner R.A. The structure of an antigenic determinant in a protein. Cell 37 (1984) 767