The effects of pulsed electromagnetic fields on the cellular activity of SaOS-2 cells

Bioelectromagnetics

Volumn 29, Issue 2, 2008, Pages 125-132

  Martino, Carlos F ^a   Belchenko, Dmitry ^a   Ferguson, Virginia ^a   Nielsen Preiss, Sheila ^b   Qi, H Jerry ^a  

^a UCB 450   (United States)

^b UNIVERSITY OF COLORADO   (United States)

Author keywords

Electromagnetic stimulation; Mineralization; PEMFs; SaOS 2

Indexed keywords

BONE; ELECTROMAGNETIC FIELDS; METABOLISM; MINERALOGY; PHOSPHATASES;

BONE FRACTURE; CELLULAR ACTIVITIES; ELECTROMAGNETIC FIELDS STIMULATIONS; ELECTROMAGNETIC STIMULATION; MINERALISATION; PULSED ELECTROMAGNETIC FIELDS; REPETITIVE PULSE; SAOS-2; SAOS-2 CELLS; WAVEFORMS;

CELL PROLIFERATION;

ALIZARIN RED S; ALKALINE PHOSPHATASE;

ARTICLE; BONE MINERALIZATION; CELL ACTIVITY; CELL COUNT; CELL METABOLISM; CELL PROLIFERATION; CONTROLLED STUDY; ELECTROMAGNETIC FIELD; FRACTURE HEALING; FRACTURE NONUNION; HUMAN; HUMAN CELL; OSSIFICATION; OSTEOBLAST; PRIORITY JOURNAL; PROTEIN EXPRESSION; WAVEFORM; WESTERN BLOTTING;

EID: 38949086548     PISSN: 01978462     EISSN: 1521186X     Source Type: Journal    
DOI: 10.1002/bem.20372     Document Type: Article

References (25)

1. Ayers R, Nielsen-Preiss S, Ferguson V, Gotolli G, Moore JJ, Kleebe H-J. 2006. Osteoblast-like cell mineralization induced by multiphasic calcium phosphate ceramic. Mater Sci Eng C26 6(8):1333-1337.
2. Bassett CA, Pawluk RJ, Pilla AA. 1974. Acceleration of fracture repair by electromagnetic fields. A surgically noninvasive method. Ann N Y Acad Sci 238:242-262.
3. Bassett CA, Pilla AA, Pawluk RJ. 1977. A non-operative salvage of surgically-resistant pseudarthroses and non-unions by pulsing electromagnetic fields. A preliminary report. Clin Orthop Relat Res 124:128-143.
4. Bassett CA, Mitchell SN, Norton L, Pilla A. 1978. Repair of non-unions by pulsing electromagnetic fields. Acta Orthop Belg 44(5):706-724.
5. Baumert U, Golan I, Becker B, Hrala BP, Redlich M, Roos HA, Palmon A, Reichenberg E, Mussig D. 2004. Pressure simulation of orthodontic force in osteoblasts: A pilot study. Orthod Craniofac Res 7(1):3-9.
6. Becker RO, Spadaro JA, Marino AA. 1977. Clinical experiences with low intensity direct current stimulation of bone growth. Clin Orthop Relat Res 124:75-83.
7. Brighton CT, Friedenberg ZB, Zemsky LM, Pollis PR. 1975. Direct-current stimulation of non-union and congenital pseudarthrosis. Exploration of its clinical application. J Bone Joint Surg Am 57(3):368-377.
8. Brighton CT, Friedenberg ZB, Mitchell EI, Booth RE. 1977. Treatment of nonunion with constant direct current. Clin Orthop Relat Res 124:106-123.
9. Chang WH, Chen LT, Sun JS, Lin FH. 2004. Effect of pulse-burst electromagnetic field stimulation on osteoblast cell activities. Bioelectromagnetics 25(6):457-465.
10. Gillette JM, Nielsen-Preiss SM. 2004. The role of annexin 2 in osteoblastic mineralization. J Cell Sci 117(Pt 3):441-449.
11. Granet C, Boutahar N, Vico L, Alexandre C, Lafage-Proust MH. 2001. MAPK and SRC-kinases control EGR-1 and NF-kappa B inductions by changes in mechanical environment in osteoblasts. Biochem Biophys Res Commun 284(3):622-631.
12. Granet C, Vico AG, Alexandre C, Lafage-Proust MH. 2002. MAP and src kinases control the induction of AP-1 members in response to changes in mechanical environment in osteoblastic cells. Cell Signal 14(8):679-688.
13. Gregory CA, Gunn WG, Peister A, Prockop DJ. 2004. An Alizarin red-based assay of mineralization by adherent cells in culture: Comparison with cetylpyridinium chloride extraction. Anal Biochem 329(1):77-84.
14. Hartig M, Joos U, Wiesmann HP. 2000. Capacitively coupled electric fields accelerate proliferation of osteoblast-like primary cells and increase bone extracellular matrix formation in vitro. Eur Biophys J 29(7):499-506.
15. Heermeier K, Spanner M, Trager J, Gradinger R, Strauss PG, Kraus W, Schmidt J. 1998. Effects of extremely low frequency electromagnetic field (EMF) on collagen type I mRNA expression and extracellular matrix synthesis of human osteoblastic cells. Bioelectromagnetics 19(4):222-231.
16. Jensh RP, Brent RL. 1966. Rapid schedules for KOH clearing and alizarin red S staining of fetal rat bone. Stain Technol 41(3): 179-183.
17. Liu X, Zhang X, Luo ZP. 2005. Strain-related collagen gene expression in human osteoblast-like cells. Cell Tissue Res 322(2):331-334.
18. Palmer AE, Tsien RY. 2006. Measuring calcium signaling using genetically targetable fluorescent indicators. Nat Protoc 1(3):1057-1065.
19. Pilla AA. 2006. Mechanisms and therapeutic applications of time varying and static magnetic fields. In: Barnes F, Greenebaum B, editors. Biological and medical aspects of electromagnetic fields. Boca Raton, FL: CRC Press. pp. 351-411.
20. Pilla AA, Sechaud P, McLeod BR. 1983. Electrochemical and electrical aspects of low-frequency electromagnetic current induction in biological systems. J Biol Phys 11(2):51-58.
21. Puchtler H, Meloan SN, Terry MS. 1969. On the history and mechanism of alizarin and alizarin red S stains for calcium. J Histochem Cytochem 17(2):110-124.
22. Spadaro JA. 1997. Mechanical and electrical interactions in bone remodeling. Bioelectromagnetics 18(3):193-202.
23. Spadaro JA, Mino DE, Chase SE, Werner FW, Murray DG. 1986. Mechanical factors in electrode-induced osteogenesis. J Orthop Res 4(1):37-44.
24. Stanford CM, Jacobson PA, Eanes ED, Lembke LA, Midura RJ. 1995. Rapidly forming apatitic mineral in an osteoblastic cell line (UMR106-01 BSP). J Biol Chem 270(16):9420-9428.
25. Wiesmann H, Hartig M, Stratmann U, Meyer U, Joos U. 2001. Electrical stimulation influences mineral formation of osteoblast-like cells in vitro. Biochim Biophys Acta 1538(1):28-37.