A gram-scale separation of glucosinolates from an oil-pressed residue of rapeseeds using slow rotary countercurrent chromatography

Separation and Purification Technology

Volumn 59, Issue 3, 2008, Pages 294-298

  Du, Qizhen ^a   Fang, Jie ^a   Gao, Shijun ^a   Zeng, Qiaoyun ^b   Mo, Cehui ^c  

^a ZHEJIANG GONGSHANG UNIVERSITY   (China)

^b SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

^c JINAN UNIVERSITY   (China)

Author keywords

Glucosinolates; Rapeseeds; Separation; Slow rotary countercurrent chromatography

Indexed keywords

ACETONITRILE; BUTENES; CHROMATOGRAPHIC ANALYSIS; MASS SPECTROMETRY; SEPARATION; SOLVENTS;

GLUCOSINOLATES; RAPESEEDS; SLOW ROTARY COUNTER CURRENT CHROMATOGRAPHY;

VEGETABLE OILS;

BRASSICA NAPUS;

EID: 38849145764     PISSN: 13835866     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.seppur.2007.06.021     Document Type: Article

References (12)

1. Fahey J.W., and Talalay P. Antioxidant functions of sulforaphane: a potent inducer of phase II detoxification enzymes. Food Chem. Toxicol. 37 (1999) 973
2. Fahey J.W., Haristoy X., Dolan P.M., Kensler T.W., Scholtus I., Stephenson K.K., Talalay P., and Lozniewski A. Sulforaphane inhibits extracellular, intracellular, and antibiotic-resistant strains of Helicobacter pylori and prevents benzo[a]pyrene-induced stomach tumors. Proc. Natl. Acad. Sci. 99 (2002) 7610-7615
3. Talalay P., and Fahey J.W. Phytochemicals from cruciferous plants protect against cancer by modulating carcinogen metabolism. J. Nutr. 131 (2001) 3027S
4. Shapiro T.A., Fahey J.W., Wade K.L., Stephenson K.K., and Talalay P. Disposition of chemoprotective glucosinolates and isothiocyanates of broccoli sprouts. Cancer Epidemiol. Biomark. Prevent. 10 (2001) 501
5. Halkier B.A. Glucosinolates. In: Ikan R. (Ed). Naturally Occurring Glycosides: Chemistry Distribution and Biological Properties (1999), John Wiley & Sons 193-223
6. Peterka S., and Fenwick G.R. The use of flash chromatography for the isolation and purification of glucosinolates (mustard oil glycosides). Fat Sci. Technol. 90 (1988) 61
7. Thies W. Isolation of sinigrin and glucotropaeolin from cruciferous seeds. Fat Sci. Technol. 90 (1988) 311
8. Fahey J.W., Wadea K.L., Stephensona K.K., and Chou F.E. Separation and purification of glucosinolates from crude plant homogenates by high-speed countercurrent chromatography. J. Chromatogr. A 996 (2003) 85
9. Du Q., Wu P., and Ito Y. Low-speed rotary countercurrent chromatography using converted multilayer helical tube for industrial separation. Anal. Chem. 72 (2000) 3363
10. Du Q., Jerz G., He Y., Li L., Xu Y., Zhang Q., Zheng Q., Winterhalter P., and Ito Y. Semi-industrial isolation of salicin and amygdalin from plant extracts using slow rotary countercurrent chromatography. J. Chromatogr. A 1074 (2005) 43
11. Prestera T., Fahey J.W., Holtzclaw W.D., Abeygunawardana C., Kachinski J.L., and Talalay P. Comprehensive chromatographic and spectroscopic methods for the separation and identification of intact glucosinolates. Anal. Biochem. 239 (1996) 168
12. Cox I.J., Hanley A.B., Belton P.S., and Fenwick G.R. NMR spectra (1H, 13C) of glucosinotates. Carbohydr. Res. 132 (1984) 323