Improved pulsed-field gel electrophoresis procedure for the analysis of Flavobacterium columnare isolates previously affected by DNA degradation

Veterinary Microbiology

Volumn 128, Issue 1-2, 2008, Pages 207-212

  Soto, Esteban ^a,b   Mauel, Michael ^a,b   Lawrence, Mark ^b  

^a Thad Cochran National Warmwater Aquaculture Center   (United States)

^b MISSISSIPPI STATE UNIVERSITY   (United States)

Author keywords

DNA degradation; Flavobacterium columnare; Formaldehyde fixation; PFGE

Indexed keywords

DEOXYRIBONUCLEASE; FORMALDEHYDE;

ARTICLE; BACTERIUM IDENTIFICATION; CELL ISOLATION; DNA DEGRADATION; FISH; FLAVOBACTERIUM; FLAVOBACTERIUM COLUMNARE; HOST; ISOLATION PROCEDURE; NONHUMAN; PULSED FIELD GEL ELECTROPHORESIS;

ANIMALS; BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA, BACTERIAL; ELECTROPHORESIS, GEL, PULSED-FIELD; FISH DISEASES; FISHES; FLAVOBACTERIACEAE INFECTIONS; FLAVOBACTERIUM; FORMALDEHYDE; PHYLOGENY; VARIATION (GENETICS);

BACTERIA (MICROORGANISMS); FLAVOBACTERIUM COLUMNARE;

EID: 38849136512     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2007.10.001     Document Type: Article

References (21)

1. Arias C.R., Welker T.L., Shoemaker C.A., Abernathy J.W., and Klesius P.H. Genetic fingerprinting of Flavobacterium columnare isolates from cultured fish. J. Appl. Microbiol. 97 (2004) 421-428
2. Barrett T.J., Gerner-Smidt P., and Swaminathan B. Interpretation of pulsed-field gel electrophoresis patterns in foodborne disease investigations and surveillance. Foodborne Pathog. Dis. 3 (2006) 20-31
3. Darwish A.M., and Ismaiel A.A. Genetic diversity of Flavobacterium columnare examined by restriction fragment polymorphism and sequencing of the 16S ribosomal RNA gene and the 16S-23S rDNA spacer. Mol. Cell. Probes 19 (2005) 267-274
4. Farmer, B., 2004. Improved methods for the isolation and characterization of Flavobacterium columnare. Thesis. Louisiana State University, Baton Rouge, LA.
5. Figueiredo H.C.P., Klesius P.H., Arias C.R., Evans J., Shoemaker C.A., Pereira D.J., and Peixoto M.T. Isolation and characterization of strains of Flavobacterium columnare from Brazil. J. Fish Dis. 28 (2005) 199-204
6. Hielm S., Bjorkroth J., Hyytia E., and Korkeala H. Genomic analysis of Clostridium botulinum Group II by pulsed-field gel electrophoresis. J. Appl. Environ. Microbiol. 64 (1998) 703-708
7. Hughes V.M., Stevenson K., and Sharp J.M. Improved preparation of high molecular weight DNA for pulsed-field gel electrophoresis from Mycobacteria. J. Microbiol. Methods 44 (2001) 209-215
8. Koort J.M.K., Lukinmaa S., Rantala M., Unkila E., and Siitonen A. Technical improvement to prevent DNA degradation of enteric pathogens in pulsed-field gel electrophoresis. J. Clin. Microbiol. 40 (2002) 3497-3498
9. Leclair D., Pagotto F., Farber J.M., Cadieux B., and Austin J.W. Comparison of DNA fingerprinting methods for use in investigation of type E botulism outbreaks in the Canadian Arctic. J. Clin. Microbiol. 44 (2006) 1635-1644
10. Matsheka M.I., Elisha B.G., Lastovica A.L., and On S.L.W. Genetic heterogeneity of Campylobacter concisus determined by pulsed field gel electrophoresis-based macrorestriction profiling. FEMS Microbiol. Lett. 211 (2002) 17-22
11. Olivares-Fuster O., Shoemaker C.A., Klesius P.H., and Arias C.R. Molecular typing of isolates of the fish pathogen, Flavobacterium columnare, by single-strand conformation polymorphism analysis. FEMS Microbiol. Lett. 269 1 (2007) 63-69 (Epub 2007 Jan 11)
12. Plumb J.A. Health Maintenance and Principal Microbial Diseases of Cultured Fishes (1999), Iowa State University Press, Ames, Iowa
13. Schneck J.L., and Caslake L.F. Genetic diversity of Flavobacterium columnare isolated from fish collected from warm and coldwater. J. Fish Dis. 29 (2006) 245-248
14. Shoemaker C.A., Shelby R.A., and Klesius P.H. Development of an indirect ELISA to detect humoral response to Flavobacterium columnare infection of channel catfish, Ictalurus punctatus. J. Appl. Aquacult. 14 (2003) 42-52
15. Soto E., Lawrence, M., Karsi, A., Mauel, M., in press. Genetic and virulence characterization of Flavobacterium columnare from channel catfish (Ictalurus punctatus). J. Appl. Microbiol.
16. Spigaglia P., Cardines R., Rossi S., Menozzi M.G., and Mastrantonio P. Molecular typing and long term comparison of Clostridium difficile strains by pulsed-field gel electrophoresis and PCR-ribotyping. J. Med. Microbiol. 50 (2001) 407-414
17. Thomas-Jinu S., and Goodwin A.E. Morphological and genetic characteristics of Flavobacterium columnare isolates: correlation with virulence in fish. J. Fish Dis. 27 (2004) 29-35
18. Triyanto, and Wakabayashi H. Genotypic diversity of strains of Flavobacterium columnare from diseased fishes. Fish Pathol. 34 (1999) 65-71
19. Welker T.L., Shoemaker C.A., Arias C.R., and Klesius P.H. Transmission and detection of Flavobacterium columnare in channel catfish Ictalurus punctatus. Dis. Aquat. Organ. 63 (2005) 129-138
20. Wong H.-C., and Kuo C.-J. An improved procedure for the pulsed-field gel electrophoresis analysis of Vibrio vulnificus. J. Food Drug Anal. 14 (2006) 215-217
21. Zhang Y., Yakrus M.A., Graviss E.A., Williams-Bouyer N., Turenne C., Kabani A., and Wallace R.J. Pulsed-field gel electrophoresis study of Mycobacterium abscessus isolates previously affected by DNA degradation. J. Clin. Microbiol. 42 (2004) 5582-5587