An improved procedure for the pulsed-field gel electrophoresis analysis of Vibrio vulnificus

Journal of Food and Drug Analysis

Volumn 14, Issue 2, 2006, Pages 215-217

  Wong, Hin Chung ^a   Kuo, Cheng Ju ^a  

^a SOOCHOW UNIVERSITY   (Taiwan)

Author keywords

DNase; Pulsed field gel electrophoresis; Vibrio vulnificus

Indexed keywords

BACTERIAL DNA; BACTERIAL ENZYME; EDETIC ACID; PROTEINASE K; SUCROSE;

ARTICLE; BACTERIAL CELL; BACTERIAL CHROMOSOME; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CONTROLLED STUDY; CYTOPLASM; ENZYME MECHANISM; NONHUMAN; OUTER MEMBRANE; PULSED FIELD GEL ELECTROPHORESIS; VIBRIO VULNIFICUS;

BACTERIA (MICROORGANISMS); VIBRIO VULNIFICUS;

EID: 33747435514     PISSN: 10219498     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Linkous, D. A. and Oliver, J. D. 1999. Pathogenesis of Fibrio vulnificus. FEMS Microbiol. Lett. 174: 207-214.
2. Jackson, J. K., Murphree, R. L. and Tamplin, M. L. 1997. Evidence that mortality from Vibrio vulnificus infection results from single strains among heterogeneous populations in shellfish. J. Clin. Microbiol. 35: 2098-2101.
3. Hoi, L., Dalsgaard, L, DePaola, A., Siebeling, R. J. and Dalsgaard, A. 1998. Heterogeneity among isolates of Vibrio vulnificus recovered from Eels (Anguilla angulla) in Denmark. Appl. Environ. Microbiol. 64: 4676-4682.
4. Arias, C. R., Verdonck, L., Swings, J., Garay, E. and Aznar, R. 1997. Intraspecific differentiation of Vibrio vulnificus biotypes by amplified fragment length polymorphism and ribotyping. Appl. Environ. Microbiol. 63: 2600-2606.
5. Aznar, R., Ludwig, W. and Schleifer, K. H. 1993. Ribotyping and randomly amplified polymorphic DNA analysis of Vibrio vulnificus biotypes. Syst. Appl. Microbiol. 16: 303-309.
6. Hoi, L., Dalsgaard, A., Larsen, J. L., Warner, J. M. and Oliver, J. D. 1997. Comparison of ribotyping and randomly amplified polymorphic DNA PCR for characterization of Vibrio vulnificus. Appl. Environ. Microbiol. 63: 1674-1678.
7. Mahalingam, S., Cheong, Y. M., Kan, S., Yassin, R. M., Vadivelu, J. and Pang, T. 1994. Molecular epidemiologic analysis of Vibrio cholerae O1 isolates by pulsed-field gel electrophoresis. J. Clin. Microbiol. 32: 2975-2979.
8. Skov, M. N., Pedersen, K. and Larsen, J. L. 1995. Comparison of pulsed-field gel electrophoresis, ribotyping, and plasmid profiling for typing of Vibrio anguillarum serovar O1. Appl. Environ. Microbiol. 61: 1540-1545.
9. Wong, H., Lu, K. T., Pan, T. M., Lee, C. L. and Shih, D. Y. C. 1996. Subspecies typing of Vibrio parahae-molyticus by pulsed-field gel electrophoresis. J. Clin. Microbiol. 34: 1535-1539.
10. Wong, H. C., Chen, S. Y., Chen, M. Y., Oliver, J. D., Hor, L. I. and Tsai, W. C. 2004. Pulsed-field gel electrophoresis analysis of Vibrio vulnificus strains isolated from Taiwan and United States. Appl. Environ. Microbiol 70: 5153-5158.
11. Watanabe, H., Miyoshi, S., Kawase, T., Tomochika, K. and Shinoda, S. 2004. High growing ability of Vibrio vulnificus biotype 1 is essential for production of a toxic metalloprotease causing systemic diseases in humans. Microb. Pathog. 36: 117-123.
12. Testa, J., Daniel, L. W. and Kreger, A. S. 1984. Extracellular phospholipase A2 and lysophospholipase produced by Vibrio vulnificus. Infect. Immun. 45: 458-463.
13. Wright, A. C. and Morris, J. G., Jr. 1991. The extracellular cytolysin of Vibrio vulnificus: Inactivation and relationship to virulence in mice. Infect. Immun. 59: 192-197.
14. Biosca, E. G., Oliver, J. D. and Amaro, C. 1996. Phenotypic characterization of Vibrio vulnificus biotype 2, a lipopolysaccharide-based homogeneous O serogroup within Vibrio vulnificus. Appl. Environ, Microbiol. 62: 918-927.
15. Focareta, T. and Manning, P. A. 1991. Distinguishing between the extracellular DNases of Vibrio cholerae and development of a transformation system. Mol. Microbiol. 5: 2547-2555.
16. Moulard, M., Condemine, G. and Robert-Baudouy, J. 1993. Characterization of the nucM gene coding for a nuclease of the phytopathogenic bacteria Erwinia chrysanthemi. Mol. Microbiol. 8: 685-695.
17. Wu, S. I., Lo, S. K., Shao, C. P., Tsai, H. W. and Hor, L. I. 2001. Cloning and characterization of a periplasmic nuclease of Vibrio vulnificus and its role in preventing uptake of foreign DNA. Appl. Environ. Microbiol 67: 82-88.
18. Matsheka, M. I., Elisha, B. G., Lastovica, A. L. and On, S. L. 2002. Genetic heterogeneity of Campylobacter concisus determined by pulsed field gel electrophoresis-based macrorestriction profiling. FEMS Microbiol. Lett. 211: 17-22.
19. Murase, T., Nagato, M., Shirota, K., Katoh, H. and Otsuki, K. 2004. Pulsed-field gel electrophoresis-based subtyping of DNA degradation-sensitive Salmonella enterica subsp. enterica serovar Livingstone and serovar Cerro isolates obtained from a chicken layer farm. Vet. Microbiol. 99: 139-143.
20. Marshall, S., Clark, C. G., Wang, G., Mulvey, M., Kelly, M. T. and Johnson, W. M. 1999. Comparison of molecular methods for typing Vibrio parahaemolyticus. J. Clin. Microbiol. 37: 2473-2478.
21. Koort, J. M., Lukinmaa, S., Rantala, M., Unkila, E. and Siitonen, A. 2002. Technical improvement to prevent DNA degradation of enteric pathogens in pulsed-field gel electrophoresis. J. Clin. Microbiol. 40: 3497-3498.
22. Corkill, J. E., Graham, R., Hart, C. A. and Stubbs, S. 2000. Pulsed-field gel electrophoresis of degradation-sensitive DNAs from Clostridium difficile PCR ribotype 1 strains. J. Clin. Microbiol. 38: 2791-2792.
23. Hielm, S., Bjorkroth, J., Hyytia, E. and Korkeala, H. 1998. Genomic analysis of Clostridium botulinum group II by pulsed-field get electrophoresis. Appl. Environ. Microbiol. 64: 703-708.