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Experimental details and additional data on the adsorption of peroxidases to QDs, stoichiometric variations of HRP:QD ratios, formation of hydroxyl radicals by QD irradiation, and oxidation of various peroxidase substrates are available in the Supporting Information
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Depending on the species and pH of the solution, the lifetime of radicals can range from milliseconds to several hours. In the case of superoxide radicals, lifetimes can extend several hours at high pH values in the absence of metal ions. For example, see
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Depending on the species and pH of the solution, the lifetime of radicals can range from milliseconds to several hours. In the case of superoxide radicals, lifetimes can extend several hours at high pH values in the absence of metal ions. For example, see: L. Y. Zang, H. P. Misra, J Biol. Chem. 1992, 267, 23 601-23 608,
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[13] it was found that physical adsorption of enzyme to QDs is not mandatory for the combined photocatalytic action of the two components. Together with the observation that pre-irradiated QDs can also activate peroxidases, these results suggest that diffusional transport of ROS is sufficient for activating the enzymes.
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[13] it was found that physical adsorption of enzyme to QDs is not mandatory for the combined photocatalytic action of the two components. Together with the observation that pre-irradiated QDs can also activate peroxidases, these results suggest that diffusional transport of ROS is sufficient for activating the enzymes.
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-1 (CYP152A1).
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-1 (CYP152A1).
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