Insights into the structural basis of the pH-dependent dimer-tetramer equilibrium through crystallographic analysis of recombinant Diocleinae lectins

Biochemical Journal

Volumn 409, Issue 2, 2008, Pages 417-428

  Nagano, Celso S ^a   Calvete, Juan J ^a   Barettino, Domingo ^a   Pérez, Alicia ^a   Cavada, Benildo S ^b   Sanz, Libia ^a  

^a INSTITUTO DE BIOMEDICINA DE VALENCIA   (Spain)

^b FEDERAL UNIVERSITY OF CEARÁ   (Brazil)

Author keywords

Dimer tetramer equilibrium; Diocleinae lectin; Recombinant lectin; Site directed mutagenesis; Synthetic gene; X ray crystal structure

Indexed keywords

CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; ESCHERICHIA COLI; GENE ENCODING; MUTAGENESIS; PH EFFECTS;

DIMER-TETRAMER EQUILIBRIUM; DIOCLEINAE LECTIN; RECOMBINANT LECTIN; SYNTHETIC GENES; X-RAY CRYSTAL STRUCTURE;

DIMERS;

CARBOHYDRATE BINDING PROTEIN; DIMER; LECTIN; RECOMBINANT PROTEIN; SYNTHETIC DNA; TETRAMER;

ALPHA CHAIN; ARTICLE; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; DIOCLEA; DIOCLEA GRANDIFLORA; DIOCLEA GUIANENSIS; ESCHERICHIA COLI; GENE MUTATION; HYPOTHESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; DIOCLEA; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; INTRONS; MODELS, GENETIC; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PLANT LECTINS; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP;

DIOCLEA GRANDIFLORA; DIOCLEA GUIANENSIS; ESCHERICHIA COLI;

EID: 38749151328     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20070942     Document Type: Article

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