Electrospray ionization mass spectrometry of soybean lipoxygenases: N-terminal acetylation, chemical modification, and solution conformation

Proteins: Structure, Function and Genetics

Volumn 70, Issue 3, 2008, Pages 650-658

  Peariso, Amber M ^a   Nicholson, Kellie M ^a   Jones, R Benjamin ^b   Green Church, Kari B ^b   Funk Jr , Max O ^a  

^a UNIVERSITY OF TOLEDO   (United States)

^b OHIO STATE UNIVERSITY   (United States)

Author keywords

Acetylation; Conformation; Electrospray; Lipoxygenase; Mass; Modification; N terminal; Post translational; Unfolding

Indexed keywords

ACETIC ACID; ACYLTRANSFERASE; APOPROTEIN; CUMENE HYDROPEROXIDE; LIPOXYGENASE; OXYGEN; POLYPEPTIDE; TRYPSIN;

ACETYLATION; AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CHEMICAL MODIFICATION; CIRCULAR DICHROISM; COVALENT BOND; ELECTROSPRAY MASS SPECTROMETRY; ENZYME CONFORMATION; ENZYME DENATURATION; HYDRODYNAMICS; LIGHT SCATTERING; MEASUREMENT; MOLECULAR WEIGHT; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN FOLDING; PROTEIN PROCESSING; SEQUENCE HOMOLOGY; SOYBEAN;

ACETYLATION; HYDROGEN-ION CONCENTRATION; LIPOXYGENASE; MODELS, MOLECULAR; MOLECULAR WEIGHT; PLANT PROTEINS; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STRUCTURE, SECONDARY; SOYBEANS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

GLYCINE MAX;

EID: 38549136593     PISSN: 08873585     EISSN: 10970134     Source Type: Journal    
DOI: 10.1002/prot.21543     Document Type: Article

References (33)

1. Funk CD. Prostaglandins and leukotrienes: advances in eicosanoid biology. Science 2001;294:1871-1875.
2. Feussner I, Wasternack C. The lipoxygenase pathway. Annu Rev Plant Biol 2002;53:275-297.
3. Funk MO, Carroll RT, Thompson JF, Sands RH, Dunham WR. Role of iron in lipoxygenase catalysis, J Am Chem Soc 1990;112:5375-5376.
4. Liang ZX, Klinman JP. Structural bases of hydrogen tunneling in enzymes: progress and puzzles. Curr Opin Struct Biol 2004;14:648-655.
5. Boyington JC, Gaffney BJ, Amzel LM. The three dimensional structure of soybean lipoxygenase-1: an arachidonic acid 15-lipoxygenase. Science 1993;260:1482-1486.
6. Minor W, Steczko J, Stec B, Otwinowski Z, Bolin JT, Walter R, Axelrod B. Crystal structure of soybean lipoxgyenase L-1 at 1.4 angstrom resolution. Biochemistry 1996;35:10687-10701.
7. Skrzypczak-Jankun E, Amzel LM, Kroa BA, Funk MO. Structure of soybean lipoxygenase L3 and a comparison with its L1 isoenzyme. Prot Struct Func Genet 1997;29:15-31.
8. Oldham ML, Brash AR, Newcomer ME. Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via a C2-like domain and a structural basis for product chirality. J Biol Chem 2005;280:39545-39552.
9. Youn B, Sellhorn GE, Mirchel RJ, Gaffney BJ, Howard D, Kang CH. Crystal structures of vegetative soybean lipoxygenaseVLX-B and VLX-D, and comparisons with seed isoforms LOX-1 and LOX-3. Prot Struct Funct Bioinformat 2006;65:1008-1020.
10. Tatulian SA, Steczko J, Minor W. Uncovering a calcium-regulated membrane binding mechanism for soybean lipoxygenase-1. Biochemistry 1998;44:15481-15490.
11. Hammel M, Walther M, Prassi R, Kuhn H. Structural flexibility of the N-terminal β-barrel domain of 15-lipoxygenase-1 probed by small angle X-ray scattering. Functional consequences for activity regulation and membrane binding. J Mol Biol 2004;343:917-929.
12. Dainese E, Sabatucci A, Van Zadelhoff G, Angelucci CB, Vachette P, Veldink GA, Finazzi-Agro A, Maccarrone M. Structural stability of soybean lipoxygenase-1 in solution as probed by small angle x-ray scattering. J Mol Biol 2005;349:143-152.
13. Konermann L, Rosell FI, Mauk AG, Douglas DJ. Acid-induced denaturation of myoglobin studied by time resolved electrospray ionization mass spectrometry. Biochemistry 1997;36:6448-6454.
14. Konermann L, Douglas DJ. Acid induced unfolding of cytochrome c at different methanol concentrations: electrospray ionization mass spectrometry specifically monitors changes in the tertiary structure. Biochemistry 1997;36:12296-12302.
15. Gumerov DR, Mason AB, Kaltashov IA. Interlobe communication in human serum transferrin: metal binding and conformational dynamics investigated by electrospray ionization mass spectrometry. Biochemistry 2003;42:5421-5428.
16. Funk MO Carroll RT, Thompson JF, Dunham WR. The lipoxygenases in developing seeds, their characterization and synthesis in vitro. Plant Physiol 1986;82:1139-1144.
17. Vahedi-Faridi A, Brault PA, Shah P, Kim YW, Dunham WR, Funk MO. Interaction between non heme iron of lipoxygenases and cumene hydroperoxide: basis for enzyme activation, inactivation, and inhibition. J Am Chem Soc 2004;126:2066-2015.
18. Stone KL, Williams KR. Enzymatic digestion of proteins and HPLC peptide isolation. In: Matsudaira PT, editor. A practical guide to protein and peptide purification for microsequencing, 2nd ed. New York: Academic Press; 1993. pp 43-69.
19. Axelrod B, Cheesebrough TM, Laasko S. Lipoxygenase from soybeans. Methods Enzymol 1981;71:441-451.
20. Sudharshan E, Srinivasulu S, Appu Rao AG. pH induced domain interaction and conformational transititions of lipoxygenase-1. Biochim Biophys Acta 2000;1480:13-22.
21. Brault PA, Kariapper MST, Pham CV, Flowers RA, Gunning WT, Shah P, Funk MO. Protein micelles from lipoxygenase-3. Biomacromolecules 2002;3:649-654.
22. Shibata D, Steczko J, Dixon JE, Hermodson M, Yazdanparast R, Axelrod B. Primary structure of soybean lipoxygenase-1. J Biol Chem 1987;262:10080-10085.
23. Tercero JC, Dinman JD, Wickner RB. Yeast MAK3 N-acetyltransferase recognizes the N-terminal four amino acids of the major coat protein (gag) of the L-A double stranded RNA virus. J Bacteriol 1993;135:3192-3194.
24. Polevoda B, Sherman F. N-terminal acetyltransferases and sequence requirements for N-terminal acetylation of eukaryotic proteins. J Mol Biol 2003;325:595-622.
25. Pesaresi P, Gardner NA, Masiero S, Dietzmann A, Eichacker L, Wickner R, Salamini F, Leister D. Cytoplasmic N-terminal protein acetylation is required for efficient photosynthesis in Arabidopsis. Plant Cell 2003;15:1817-1832.
26. Setty SRG, Strochlic TI, Tong AHY, Boone C, Burd CG. Golgi targeting of ARF like GTPase Arl3p requires its N-acetylation and the integral membrane protein Sys1p. Nat Cell Biol 2004;6:414-420.
27. Pande AH, Qin ,S, Tatulian SA. Membrane fluidity is a key modulator of membrane binding, insertion and activity of 5-lipoxygenase. Biophys J 2005;88:4084-4094.
28. Clauser K. Mutation Mass Shifts. Protein Prospector. San Francisco: University of California; 2002. Available at http://prospector.ucsf.edu/ prospector/4.0.8/html/misc/mutation.htm.
29. Kramer JA, Johnson KR, Dunham WR, Sands RH, Funk MO. Position 713 is critical for catalysis but not iron binding in soybean lipoxygenase-3. Biochemistry 1994;33:15017-15022.
30. Malvezzi-Campeggi F, Rosato N, Finazzi-Agro A, Maccarrone M. Effects of denaturants on the structural properties of soybean lipoxygenase-1. Biochem Biophys Res Commun 2001;289:1295-1300.
31. Ramachandran S, Richards-Sucheck TJ, Skrzypczak-Jankun E, Wheelock MJ, Funk MO. Catalysis sensitive conformational changes in soybean lipoxygenases revealed by limited proteolysis and monoclonal antibody experiments. Biochemistry 1995;34:14868-14873.
32. Pan XM, Sheng XR, Yang SM, Zhou JM. Probing subtle acid-induced conformational changes of ribonuclease A by electrospray mass spectrometry. FEBS Lett 1997;402:25-27.
33. Bloomfield VA. Quasi-elastic light-scattering applications in biochemistry and biology. Annu Rev Biophys Bioeng 1981;10:421-450.