Optimal lens epithelial cell proliferation is dependent on the connexin isoform providing gap junctional coupling

Investigative Ophthalmology and Visual Science

Volumn 48, Issue 12, 2007, Pages 5630-5637

  White, Thomas W ^a   Gao, Yang ^b   Li, Leping ^a   Sellitto, Caterina ^a   Srinivas, Miduturu ^b  

^a STONY BROOK UNIVERSITY   (United States)

^b STATE UNIVERSITY OF NEW YORK   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; BROXURIDINE; CONNEXIN 43; CONNEXIN 46; CONNEXIN 50; GAP JUNCTION PROTEIN; ISOPROTEIN; QUININE; UNCLASSIFIED DRUG; EYE PROTEIN;

AGING; ANIMAL CELL; ANTIBODY SPECIFICITY; ARTICLE; BIOPHYSICS; CELL ASSAY; CELL DENSITY; CELL ENERGY; CELL ISOLATION; CELL LABELING; CELL NUCLEUS; CELL PROLIFERATION; CONTROLLED STUDY; CORRELATION ANALYSIS; DEVELOPMENTAL STAGE; ELECTRIC CONDUCTIVITY; EPITHELIUM CELL; GAP JUNCTION; IMMUNOCYTOCHEMISTRY; IMMUNOLOCALIZATION; LENS EPITHELIUM; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; REGULATORY MECHANISM; VOLTAGE CLAMP; WHOLE CELL; WILD TYPE; ANIMAL; CELL COMMUNICATION; CELL CULTURE; CELL DIVISION; CELL JUNCTION; CYTOLOGY; ELECTROPHYSIOLOGY; FLUORESCENT ANTIBODY TECHNIQUE; LENS; METABOLISM; MOUSE MUTANT; PATCH CLAMP; PHYSIOLOGY;

ANIMALS; BROMODEOXYURIDINE; CELL COMMUNICATION; CELL DIVISION; CELL PROLIFERATION; CELLS, CULTURED; CONNEXINS; ELECTROPHYSIOLOGY; EPITHELIAL CELLS; EYE PROTEINS; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GAP JUNCTIONS; LENS, CRYSTALLINE; MICE; MICE, KNOCKOUT; PATCH-CLAMP TECHNIQUES; PROTEIN ISOFORMS;

EID: 38549125531     PISSN: 01460404     EISSN: None     Source Type: Journal    
DOI: 10.1167/iovs.06-1540     Document Type: Article

References (42)

1. Wei CJ, Xu X, Lo CW. Connexins and cell signaling in development and disease. Annu Rev Cell Dev Biol. 2004;20:811-838.
2. Plum A, Winterhager E, Pesch J, et al. Connexin31-deficiency in mice causes transient placental dysmorphogenesis but does not impair hearing and skin differentiation. Dev Biol. 2001;231:334-347.
3. Sellitto C, Li L, White TW. Connexin50 is essential for normal postnatal lens cell proliferation. Invest Ophthalmol Vis Sci. 2004;45:3196-3202.
4. Azarnia R, Loewenstein WR. Parallel correction of cancerous growth and of a genetic defect of cell-to-cell communication. Nature. 1973;241:455-457.
5. Loewenstein WR, Rose B. The cell-cell-channel in the control of growth. Semin Cell Biol. 1992;3:59-79.
6. Naus CC. Gap junctions and tumour progression. Can J Physiol Pharmacol. 2002;80:136-141.
7. Mesnil M. Connexins and cancer. Biol Cell. 2002;94:493-500.
8. White TW, Goodenough DA, Paul DL. Targeted ablation of connexin50 in mice results in microphthalmia and zonular pulverulent cataracts. J Cell Biol. 1998;143:815-825.
9. Rong P, Wang X, Niesman I, et al. Disruption of Gja8 (alphas connexin) in mice leads to microphthalmia associated with retardation of lens growth and lens fiber maturation. Development. 2002;129:167-174.
10. White TW. Unique and redundant connexin contributions to lens development. Science. 2002;295:319-320.
11. Menko AS. Lens epithelial cell differentiation. Exp Eye Res. 2002;75:485-490.
12. Piatigorsky J. Lens differentiation in vertebrates: a review of cellular and molecular features. Differentiation. 1981;19:134-153.
13. McAvoy JW, Chamberlain CG, de Iongh RU, Hales AM, Lovicu FJ. Lens development. Eye. 1999;13(pt 3b):425-437.
14. Brewitt B, Clark JI. Growth and transparency in the lens, an epithelial tissue, stimulated by pulses of PDGF. Science. 1988;242:777-779.
15. Musil LS, Beyer EC, Goodenough DA. Expression of the gap junction protein connexin43 in embryonic chick lens: molecular cloning, ultrastructural localization, and post-translational phosphorylation. J Membr Biol. 1990;116:163-175.
16. Paul DL, Ebihara L, Takemoto LJ, Swenson KI, Goodenough DA. Connexin46, a novel lens gap junction protein, induces voltage-gated currents in nonjunctional plasma membrane of Xenopus oocytes. J Cell Biol. 1991;115:1077-1089.
17. White TW, Bruzzone R, Goodenough DA, Paul DL. Mouse Cx50, a functional member of the connexin family of gap junction proteins, is the lens fiber protein MP70. Mol Biol Cell. 1992;3:711-720.
18. Harris AL. Emerging issues of connexin-channels: biophysics fills the gap. Q Rev Biophys. 2001;34:325-472.
19. Srinivas M, Costa M, Gao Y, Fort A, Fishman GI, Spray DC. Voltage dependence of macroscopic and unitary currents of gap junction-channels formed by mouse connexin50 expressed in rat neuroblastoma cells. J Physiol. 1999;517(pt 3):673-689.
20. Srinivas M, Hopperstad MG, Spray DC. Quinine blocks specific gap junction-channel subtypes. Proc Natl Acad Sci USA. 2001;98:10942-10947.
21. White TW, Bruzzone R. Intercellular communication in the eye: clarifying the need for connexin diversity. Brain Res Brain Res Rev. 2000;32:130-137.
22. Donaldson PJ, Roos M, Evans C, Beyer E, Kistler J. Electrical properties of mammalian lens epithelial gap junction-channels. Invest Ophthalmol Vis Sci. 1994;35:3422-3428.
23. Donaldson PJ, Dong Y, Roos M, Green C, Goodenough DA, Kistler J. Changes in lens connexin expression lead to increased gap junctional voltage dependence and conductance. Am J Physiol. 1995;269:C590-C600.
24. Martinez-Wittinghan FJ, Sellitto C, White TW, Mathias RT, Paul D, Goodenough DA. Lens gap junctional coupling is modulated by connexin identity and the locus of gene expression. Invest Ophthalmol Vis Sci. 2004;45:3629-3637.
25. Cruikshank SJ, Hopperstad M, Younger M, Connors BW, Spray DC, Srinivas M. Potent block of Cx36 and Cx50 gap junction-channels by mefloquine. Proc Natl Acad Sci USA. 2004;101:12364-12369.
26. Bai D, Del Corsso C, Srinivas M, Spray DC. Block of specific gap junction-channel subtypes by 2-aminoethoxydiphenyl borate (2-APB). J Pharmacol Exp Ther. 2006;319:1452-1458.
27. Dahm R, van Marle J, Prescott AR, Quinlan RA. Gap junctions containing alpha8-connexin (MP70) in the adult mammalian lens epithelium suggests a re-evaluation of its role in the lens. Exp Eye Res. 1999;69:45-56.
28. Jiang JX, White TW, Goodenough DA, Paul DL. Molecular cloning and functional characterization of chick lens fiber connexin 45.6. Mol Biol Cell. 1994;5:363-373.
29. Hopperstad MG, Srinivas M, Spray DC. Properties of gap junction-channels formed by Cx46 alone and in combination with Cx50. Biophys J. 2000;79:1954-1966.
30. Fishman GI, Moreno AP, Spray DC, Leinwand LA. Functional analysis of human cardiac gap junction-channel mutants. Proc Natl Acad Sci USA. 1991;88:3525-3529.
31. White TW, Bruzzone R, Wolfram S, Paul DL, Goodenough DA. Selective interactions among the multiple connexin proteins expressed in the vertebrate lens: the second extracellular domain is a determinant of compatibility between connexins. J Cell Biol. 1994;125:879-892.
32. Brink PR, Cronin K, Banach K, et al. Evidence for heteromeric gap junction-channels formed from rat connexin43 and human connexin37. Am J Physiol. 1997;273:C1386-C1396.
33. Zhou M, Leiberman J, Xu J, Lavker RM. A hierarchy of proliferative cells exists in mouse lens epithelium: implications for lens maintenance. Invest Ophthalmol Vis Sci. 2006;47:2997-3003.
34. Reddan JR, Kuck JF, Dziedzic DC, Kuck KD, Reddan PR, Wasielewski P. Establishment of lens epithelial cell lines from Emory and cataract resistant mice and their response to hydrogen peroxide. Lens Eye Toxic Res. 1989;6:687-701.
35. McAvoy JW, Chamberlain CG. Fibroblast growth factor (FGF) induces different responses in lens epithelial cells depending on its concentration. Development. 1989;107:221-228.
36. Hyatt GA, Beebe DC. Regulation of lens cell growth and polarity by an embryo-specific growth factor and by inhibitors of lens cell proliferation and differentiation. Development. 1993;117:701-709.
37. Le AC, Musil LS. A novel role for FGF and extracellular signal-regulated kinase in gap junction-mediated intercellular communication in the lens. J Cell Biol. 2001;154:197-216.
38. Zatechka SD Jr, Lou MF. Studies of the mitogen-activated protein kinases and phosphatidylinositol-3 kinase in the lens, 1: the mitogenic and stress responses. Exp Eye Res. 2002;74:703-717.
39. Goldberg GS, Valiunas V, Brink PR. Selective permeability of gap junction-channels. Biochim Biophys Acta. 2004;1662:96-101.
40. Chung J, Berthoud VM, Novak L, et al. Transgenic overexpression of connexin50 induces cataracts. Exp Eye Res. 2007;84:513-528.
41. Martinez-Wittinghan FJ, Sellitto C, Li L, et al. Dominant cataracts result from incongruous mixing of wild-type lens connexins. J Cell Biol. 2003;161:969-978.
42. Cooper K, Rae JL, Gates P. Membrane and junctional properties of dissociated frog lens epithelial cells. J Membr Biol. 1989;111:215-227.