Enzymatic ligation assisted by nucleases: Simultaneous ligation and digestion promote the ordered assembly of DNA

Nature Protocols

Volumn 2, Issue 9, 2007, Pages 2198-2202

  Cost, Gregory J ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b SANGAMO BIOSCIENCES INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; NUCLEASE; DNA; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; RESTRICTION ENDONUCLEASE;

ARTICLE; DNA PURIFICATION; DNA RESTRICTION; ENZYMATIC LIGATION ASSISTED BY NUCLEASES; ENZYME MECHANISM; GENE CONSTRUCT; GENETIC ENGINEERING; METHODOLOGY; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DEGRADATION; QUANTITATIVE GENETICS; RESTRICTION FRAGMENT; BIOSYNTHESIS; CHEMISTRY; GENETIC PROCEDURES;

DNA; DNA LIGASES; DNA RESTRICTION ENZYMES; GENETIC TECHNIQUES;

EID: 38449122613     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.325     Document Type: Article

References (3)

1. Cost, G.J. & Cozzarelli, N.R. Directed assembly of DNA molecules via simultaneous ligation and digestion. Biotechniques 42, 84-89 (2007).
2. Bustamante, C., Bryant, Z. & Smith, S.B. Ten years of tension: single-molecule DNA mechanics. Nature 421, 423-427 (2003).
3. Kodumal, S.J. et al. Total synthesis of long DNA sequences: synthesis of a contiguous 32-kb polyketide synthase gene cluster. Proc. Natl. Acad. Sci. USA 101, 15573-15578 (2004).