Competitive pcr-elisa protocols for the quantitative and the standardized detection of viral genomes

Nature Protocols

Volumn 2, Issue 10, 2007, Pages 2511-2519

  Musiani, Monica ^a   Gallinella, Giorgio ^a   Venturoli, Simona ^a   Zerbini, Marialuisa ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

PRIMER DNA;

ARTICLE; CLASSIFICATION; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETICS; HYBRIDIZATION; ISOLATION AND PURIFICATION; METHODOLOGY; PARVOVIRUS; POLYMERASE CHAIN REACTION; STANDARD; VIRUS GENOME;

DNA PRIMERS; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENOME, VIRAL; HYBRIDIZATION, GENETIC; PARVOVIRUS B19, HUMAN; POLYMERASE CHAIN REACTION;

EID: 38449120344     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.312     Document Type: Article

References (18)

1. Clementi, M., Menzo, S., Manzin, A. & Bagnarelli, P. Quantitative molecular methods in virology. Arch. Virol. 140, 1523-1539 (1995).
2. Gallinella, G. et al. Quantitation of parvovirus B19 DNA sequences by competitive PCR: differential hybridization of the amplicons and immunoenzymatic detection on microplate. Mol. Cell. Probes 11, 127-133 (1997).
3. Zerbini, M. et al. Standardization of a PCR-ELISA assay in serum samples: diagnosis of active parvovirus B19 infection. J. Med. Virol. 59, 239-244 (1999).
4. Fini, F., Gallinella, G., Girotti, S., Zerbini, M. & Musiani, M.: Development of a chemiluminescence competitive PCR for the detection and quantification of Parvovirus B19 DNA using a microplate luminometer. Clin. Chem. 45, 1391-1396 (1999).
5. Musiani, M., Venturoli, S., Gallinella, G. & Zerbini, M.: Qualitative PCR-ELISA for the detection and typing of viral genomes. Nat. Protoc. 2, 2502-2510 (2007).
6. Bezold, G. et al. Quantitation of herpes simplex DNA in blood during aciclovir therapy with competitive PCR ELISA. Dermatology 201, 296-299 (2000).
7. Peter, J.B. & Sevall, J.S. Molecular-based methods for quantifying HIV viral load. AIDS Patient Care STDS 18, 75-79 (2004).
8. Koralnik, I.J., Boden, D., Mai, V.X., Lord, C.I. & Letvin, N.L. JC virus DNA load in patients with and without progressive multifocal leukoencephalopathy. Neurology 52, 253-260 (1999).
9. Jungkind, D. Automation of laboratory testing for infectious diseases using the polymerase chain reaction- our past, our present, our future. J. Clin. Virol. 20, 1-6 (2001).
10. Daly, P. & Doyle, S. The development of a competitive PCR-ELISA for the detection of equine herpesvirus-1. J. Virol. Methods 107, 237-244 (2003).
11. Gallinella, G. et al. Analysis of B19 virus contamination in plasma pools for manufacturing, by using a competitive polymerase chain reaction assay. Vox Sang. 83, 324-31 (2002).
12. Manaresi, E. et al. Diagnosis and quantitative evaluation of parvovirus B19 infections by real-time PCR in the clinical laboratory. J. Med. Virol. 67, 275-281 (2002).
13. Gallinella, G. et al. Calibrated real-time PCR for evaluation of parvovirus B19 viral load. Clin. Chem. 50, 759-762 (2004).
14. Watzinger, F., Ebner, K. & Lion, T.: Detection and monitoring of virus infections by real-time PCR. Mol. Aspects Med. 27, 254-298 (2006).
15. Zerbini, M. & Musiani, M. Human parvoviruses. in Manual of Clinical Microbiology, 8th Edn. (eds. Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C. & Yolken, R.H.) 1534-1543 (American Society for Microbiology, Washington, DC, 2003).
16. Gallinella, G. et al. Relevance of B19 markers in serum samples for a diagnosis of parvovirus B19-correlated diseases. J. Med. Virol. 71, 135-139 (2003).
17. Bonvicini, F. et al.: Molecular testing for detection of in vitro infectivity of plasma pools contaminated with B19 virus. J. Med. Virol. 74, 272-276 (2004).
18. Courtney, B.C., Smith, M.M. & Henchal, E.A. Development of internal controls for probe-based nucleic acid diagnostic assays. Anal. Biochem. 270, 249-256 (1999).