Development of a chemiluminescence competitive PCR for the detection and quantification of parvovirus B19 DNA using a microplate luminometer

Clinical Chemistry

Volumn 45, Issue 9, 1999, Pages 1391-1396

  Fini, Fabiana ^a   Gallinella, Giorgio ^a   Girotti, Stefano ^a   Zerbini, Marialuisa ^a   Musiani, Monica ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS DNA;

ARTICLE; CHEMOLUMINESCENCE; CLINICAL ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; DNA SEQUENCE; ERYTHEMA INFECTIOSUM; GENE AMPLIFICATION; HUMAN; NONHUMAN; PARVOVIRUS; PATIENT MONITORING; POLYMERASE CHAIN REACTION; RISK ASSESSMENT; VIRUS GENE; VIRUS INFECTION;

DNA VIRUSES; PARVOVIRUS; RNA VIRUSES;

EID: 0032843166     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/45.9.1391     Document Type: Article

References (25)

1. Simmonds P, McOmish F, Yap PL, Chan SW, Lin CK, Dusheiko G, et al. Sequence variability in the 5' non-coding region of hepatitis C virus: identification of a new virus type and restrictions on sequence diversity. J Gen Virol 1993;74:661-8.
2. Whitby K, Garson JA. Optimisation and evaluation of a quantitative chemiluminescent polymerase chain reaction assay for hepatitis C virus RNA. J Virol Methods 1995;51:75-88.
3. Caballero OL, Villa LL, Simpson AJ. Low stringency-PCR (LS-PCR) allows entirely internally standardized DNA quantitation. Nucleic Acids Res 1995;23:192-3.
4. Clementi M, Menzo S, Manzin A, Bagnarelli P. Quantitative molecular methods in virology. Arch Virol 1995;140:1523-39.
5. Haberhausen G, Pinsl J, Kuhn CC, Markert-Hahn C. Comparative study of different standardization concepts in quantitative competitive reverse transcription-PCR assays. J Clin Microbiol 1998;36:628-33.
6. Mokkapatti R, Conn RA, Carcillo JA, Romkes M, Jackson EK. Quantification of G protein mRNA using reverse transcription and competitive PCR with a colorimetric microplate assay. Mol Cell Probes 1998;12:15-25.
7. Dassi C, Signorini S, Gerthoux P, Cazzaniga M, Brambilla P. Cytochrome P450 1B1 mRNA measured in blood mononuclear cells by quantitative reverse transcription-PCR. Clin Chem 1998;44:2416-21.
8. Jensen LE, Whitehead AS. Competitive reverse transcription polymerase chain reaction for quantifying pre-mRNA and mRNA of major acute phase proteins. J Immunol Methods 1998;215:45-58.
9. Gallinella G, Zerbini M, Musiani M, Venturoli S, Gentilomi G, Manaresi E. Quantitation of parvovirus B19 DNA sequences by competitive PCR: differential hybridization of the amplicons and immunoenzymatic detection on microplate. Mol Cell Probes 1997;11:127-33.
10. Zerbini M, Musiani M. Human parvoviruses. In; Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, eds. Manual of clinical microbiology. 7th ed. Washington, DC: American Society for Microbiology, 1999:1089-98.
11. Erhardt A, Schaefer S, Athanassiou N, Kann M, Gerlich WH. Quantitative assay of PCR-amplified hepatitis B virus DNA using a peroxidase-labelled DNA probe and enhanced chemiluminescence. J Clin Microbiol 1996;34:1885-91.
12. O'Connell CD, Juhasz A, Kuo C, Reeder DJ, Hoon DS. Detection of tyrosinase mRNA in melanoma by reverse transcription-PCR and electrochemiluminescence. Clin Chem 1998;44:1161-9.
13. Martin CS, Butler L, Bronstein I. Quantitation of PCR products with chemiluminescence. Biotechniques 1995;18:908-13.
14. Girotti S, Musiani M, Pasini P, Ferri E, Gallinella G, Zerbini M, et al. Application of a low-light imaging device and chemiluminescent substrates for quantitative detection of viral DNA in hybridization reactions. Clin Chem 1995;41:1693-7.
15. Girotti S, Musiani M, Ferri E, Gallinella G, Zerbini M, Roda A, et al. Chemiluminescent immunoperoxidase assay for the dot blot hybridization detection of parvovirus B19 DNA using a low light imaging device. Anal Biochem 1996;236: 290-5.
16. Shawar RM, Humble DJ, Van Dalfsen JM, Stover CK, Hickey MJ, Steele S, et al. Rapid screening of natural products for antimycobacterial activity by using luciferase-expressing strains of Mycobacterium bovis BCG and Mycobacterium intracellulare. Antimicrob Agents Chemother 1997;41:570-4.
17. Gibellini D, Zerbini M, Musiani M, Venturoli S, Gentilomi G. La Placa M. Microplate capture hybridization of amplified parvovirus B19 DNA fragment labelled with digoxigenin. Mol Cell Probes 1993;7:453-8.
18. Kricka LJ. Chemiluminescence and bioluminescence. Anal Chem 1995;67:499R-500R.
19. Mattson DL, Bellehumeur TG. Comparison of three chemiluminescent horseradish peroxidase substrates for immunoblotting. Anal Biochem 1996;240:306-8.
20. Arhaven-Tafti H, Sugiaka K, Arghvani Z, DeSilva R, Handley RS, Sugioka Y, et al. Chemiluminescent detection of horseradish peroxidase by enzymatic generation of acridinium esters. Clin Chem 1995;41:1368-9.
21. Gallinella G, Musiani M, Zerbini M, Gentilomi G, Gibellini D, Venturoli S, La Placa M. Efficient parvovirus B19 DNA purification and molecular cloning. J Virol Methods 1993;41:203-11.
22. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual, 2nd ed. Plainview, NY: Cold Spring Harbor Laboratory, 1989.
23. Musiani M, Zerbini M, Gentilomi G, Rodorigo G, De Rosa V, Gibellini D, et al. Persistent B19 parvovirus infections in haemophilic HIV-1 infected patients J Med Virol 1995;46:103-8.
24. Musiani M, Zerbini M, Gentilomi G, Plazzi M, Gallinella G, Venturoli S. Parvovirus B19 clearance from peripheral blood after acute infection. J Infect Dis 1995;172:1360-3.
25. Musiani M, Azzi A, Zerbini M, Gibellini D, Venturoli S, Zakrzewska K, et al. Nested polymerase chain reaction assay for the detection of B19 parvovirus DNA in human immunodeficiency virus patients. J Med Virol 1993;40:157-60.