Transgenic mice expressing a fully nontoxic diphtheria toxin mutant, not CRM197 mutant, acquire immune tolerance against diphtheria toxin

Journal of Biochemistry

Volumn 142, Issue 1, 2007, Pages 105-112

  Kimura, Yasuko ^a   Saito, Michiko ^a,b   Kimata, Yukio ^a   Kohno, Kenji ^a,b  

^a NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

^b Basic Research Activities for Innovative Biosciences   (Japan)

Author keywords

ADP ribosylation; Diphtheria toxin; Elongation factor 2; HB EGF; Immune tolerance

Indexed keywords

DIPHTHERIA TOXIN; DIPHTHERIA TOXOID CRM197; ELONGATION FACTOR 2; BACTERIAL PROTEIN; BACTERIUM ANTIBODY; CRM197 (NON TOXIC VARIANT OF DIPHTHERIA TOXIN); CRM197 (NON-TOXIC VARIANT OF DIPHTHERIA TOXIN); UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOTOXICITY; HUMAN; HUMAN CELL; IMMUNOLOGICAL TOLERANCE; MAMMAL CELL; MOUSE; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; YEAST CELL; ANIMAL; CELL STRAIN COS1; CERCOPITHECUS; GENETIC TRANSFECTION; GENETICS; HELA CELL; IMMUNOLOGY; METABOLISM; TRANSGENIC MOUSE; VERO CELL;

MAMMALIA; MUS MUSCULUS;

ANIMALS; ANTIBODIES, BACTERIAL; BACTERIAL PROTEINS; CERCOPITHECUS AETHIOPS; COS CELLS; DIPHTHERIA TOXIN; HELA CELLS; HUMANS; IMMUNE TOLERANCE; MICE; MICE, TRANSGENIC; MUTATION; TRANSFECTION; VERO CELLS;

EID: 38449103471     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvm115     Document Type: Article

References (34)

1. Collier, R.-J. (1982) in ADP-ribosylation Reactions: Biology and Medicine (Hayaishi, O. and Ueda, K., eds.) pp. 575-592. Academic Press, New York
2. Collier, R.-J. (1968) Effect of diphtheria toxin on protein synthesis: inactivation of one of the transfer factors. J. Mol. Biol. 25, 83-98
3. Honjo, T., Nishizuka, Y., Hayaishi, O., and Kato, I. (1968) Diphtheria toxin-dependent adenosine diphosphate ribosylation of aminoacyl transferase II and inhibition of protein synthesis. J. Biol. Chem. 243, 3553-3555
4. Van Ness, B.-G., Howard, J.-B., and Bodley, J.-W. (1980) ADP-ribosylation of elongation factor 2 by diphtheria toxin: NMR spectra and proposed structures of ribosyldiphthamide and its hydrolysis products. J. Biol. Chem. 255, 10710-10716
5. Kohno, K., Uchida, T., Ohkubo, H., Nakanishi, S., Nakanishi, T., Fukui, T., Ohtsuka, E., Ikehara, M., and Okada, Y. (1986) Amino acid sequence of mammalian elongation factor 2 deduced from the cDNA sequence: homology with GTP-binding proteins. Proc. Natl Acad. Sci. USA 83, 4978-4982
6. Naglich, J.-G., Metherall, J.-E., Russell, D.-M., and Eidels, L. (1992) Expression cloning of a diphtheria toxin receptor: identity with a heparin-binding EGF-like growth factor precursor. Cell 69, 1051-1061
7. Saito, M., Iwawaki, T., Taya, C., Yonekawa, H., Noda, M., Inui, Y., Mekada, E., Kimata, Y., Tsuru, A., and Kohno, K. (2001) Diphtheria toxin receptor-mediated conditional and targeted cell ablation in transgenic mice. Nat. Biotechnol. 19, 746-750
8. Jung, S., Unutmaz, D., Wong, P., Sano, G., De los Santos, K., Sparwasser, T., Wu, S., Vuthoori, S., Ko, K., and Zavala, F. (2002) In vivo depletion of CD11c(+) dendritic cells abrogates priming of CD8(+) T cells by exogenous cell-associated antigens. Immunity 17, 211-220
9. Buch, T., Heppner, F.-L., Tertilt, C., Heinen, T.-J., Kremer, M., Wunderlich, F.-T., Jung, S., and Waisman, A. (2005) A Cre-inducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration. Nat. Methods 2, 419-426
10. Wharram, B.-L., Goyal, M., Wiggins, J.-E., Sanden, S.-K., Hussain, S., Filipiak, W.-E., Saunders, T.-L., Dysko, R.-C., Kohno, K., and Holzman, L.-B. (2005) Podocyte depletion causes glomerulosclerosis: diphtheria toxin-induced podocyte depletion in rats expressing human diphtheria toxin receptor transgene. J. Am. Soc. Nephrol. 16, 2941-2952
11. Chen, H., Kohno, K., and Gong, Q. (2005) Conditional ablation of mature olfactory sensory neurons mediated by diphtheria toxin receptor. J. Neurocytol. 34, 37-47
12. Luquet, S., Perez, F.-A., Hnasko, T.-S., and Palmiter, R.-D. (2005) NPY/AgRP neurons are essential for feeding in adult mice but can be ablated in neonates. Science 310, 683-685
13. Miyake, Y., Kaise, H., Isono, K., Koseki, H., Kohno, K., and Tanaka, M. (2007) Protective role of alveolar macrophages in non-inflammatory lung injury caused by selective ablation of alveolar epithelial type II cells. J. Immunol. 15, 5001-5009
14. Furukawa, N., Saito, M., Hakoshima, T., and Kohno, K. (2006) A diphtheria toxin receptor deficient in epidermal growth factor-like biological activity. J. Biochem. (Tokyo) 140, 831-841
15. Miller, J.-F., Morahan, G., and Allison, J. (1989) Immunological tolerance: new approaches using transgenic mice. Immunol. Today 10, 53-57
16. Adams, T.E. (1990) Tolerance to self-antigens in transgenic mice. Mol. Biol, Med. 7, 341-57
17. Lai, J.-C., Fukushima, A., Wawrousek, E.-F., Lobanoff, M.-C., Charukamnoetkanok, P., Smith-Gill, S.-J., Vistica, B.-P., Lee, R.-S., Egwuagu, C.-E., and Whitcup, S.-M. (1998) Immunotolerance against a foreign antigen transgenically expressed in the lens. Invest. Ophthalmol. Vis. Sci. 39, 2049-2057
18. Sly, W.S., Vogler, C., Grubb, J.H., Zhou, M., Jiang, J., Zhou, X.Y., Tomatsu, S., Bi, Y., and Snella, E.M. (2001) Active site mutant transgene confers tolerance to human beta-glucuronidase without affecting the phenotype of MPS VII mice. Proc. Natl Acad. Sci. USA 98, 2205-2210
19. Uchida, T., Pappenheimer, A.-M. Jr, and Greany, R. (1973) Diphtheria toxin and related proteins. I. Isolation and properties of mutant proteins serologically related to diphtheria toxin. J. Biol. Chem. 248, 3838-3844
20. Uchida, T., Pappenheimer, A.-M.Jr, and Harper, A.-A. (1973) Diphtheria toxin and related proteins. III. Reconstitution of hybrid "diphtheria toxin" from nontoxic mutant proteins. J. Biol. Chem. 248, 3851-3854
21. Leong, D., Coleman, K.-D., and Murphy, J.-R. (1983) Cloned DTA of diphtheria toxin is expressed and secreted into the periplasmic space of Escherichia coli K12. Science 220, 515-517
22. Niwa, H., Yamamura, K., and Miyazaki, J. (1991) Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108, 193-199
23. Iha, H. and Tsurugi, K. (1998) Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques 25, 936-938
24. Kohno, K. and Uchida, T. (1987) Highly frequent single amino acid substitution in mammalian elongation factor 2 (EF-2) results in expression of resistance to EF-2-ADP-ribosylating toxins. J. Biol. Chem. 262, 12298-12305
25. Kohno, K., Uchida, T., Mekada, E., and Okada, Y. (1985) Characterization of diphtheria-toxin-resistant mutants lacking receptor function or containing nonribosylatable elongation factor 2. Somat. Cell Mol. Genet. 11, 421-431
26. Kimata, Y. and Kohno, K. (1994) Elongation factor 2 mutants deficient in diphthamide formation show temperature-sensitive cell growth. J. Biol. Chem. 269, 13497-13501
27. Kimata, Y., Harashima, S., and Kohno, K. (1993) Expression of non-ADP-ribosylatable, diphtheria toxin-resistant elongation factor 2 in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 191, 1145-1151
28. Fu, H., Blanke, S.-R., Mattheakis, L.-C., and Collier, R.-J. (1997) Selection of diphtheria toxin active-site mutants in yeast. Rediscovery of glutamic acid-148 as a key residue. Adv. Exp. Med. Biol. 419, 45-52
29. Zhao, L. and Haslam, D.-B. (2005) A quantitative and highly sensitive luciferase-based assay for bacterial toxins that inhibit protein synthesis. J. Med. Microbiol. 54, 1023-1130
30. Carroll, S.-F., McCloskey, J.-A., Crain, P.-F., Oppenheimer, N.-J., Marschner, T.-M., and Collier, R.-J. (1985) Photoaffinity labeling of diphtheria toxin DTA with NAD: structure of the photoproduct at position 148. Proc. Natl Acad. Sci. USA 82, 7237-7241
31. Tweten, R.-K., Barbieri, J.-T., and Collier, R.-J. (1985) Diphtheria toxin. Effect of substituting aspartic acid for glutamic acid 148 on ADP-ribosyltransferase activity. J. Biol. Chem. 260, 10392-10394
32. Yates, S.-P., Jorgensen, R., Andersen, G.-R., and Merrill, A.-R. (2006) Stealth and mimicry by deadly bacterial toxins. Trends Biochem. Sci. 31, 123-133
33. Buzzi, S., Rubboli, D., Buzzi, G., Buzzi, A.-M., Morisi, C., and Pironi, F. (2004) CRM197 (nontoxic diphtheria toxin): effects on advanced cancer patients. Cancer Immunol. Immunother. 53, 1041-1048
34. Rappuoli, R., Douce, G., Dougan, G., and Pizza, M. (1995) Genetic detoxification of bacterial toxins: a new approach to vaccine development. Int. Arch. Allergy Immunol. 108, 327-333