Use of In Vitro Translation Extract Depleted in Specific Initiation Factors for the Investigation of Translational Regulation

Methods in Enzymology

Volumn 429, Issue , 2007, Pages 35-51

  Gallie, Daniel R ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INITIATION FACTOR; MESSENGER RNA; CAPPED RNA; CELL EXTRACT; LUCIFERASE; VIRUS RNA;

CELL LYSATE; HUMAN; IN VITRO SELECTION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; REVIEW; RNA SYNTHESIS; TRANSLATION INITIATION; TRANSLATION REGULATION; WESTERN BLOTTING; 5' UNTRANSLATED REGION; ARTICLE; GENETICS; METABOLISM; PHYSIOLOGY; RNA TRANSLATION; TOBACCO MOSAIC VIRUS; WHEAT;

EUKARYOTA; TRITICUM AESTIVUM;

5' UNTRANSLATED REGIONS; BLOTTING, WESTERN; CELL EXTRACTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; LUCIFERASES; PEPTIDE CHAIN INITIATION, TRANSLATIONAL; PEPTIDE INITIATION FACTORS; RNA CAPS; RNA, MESSENGER; RNA, VIRAL; TOBACCO MOSAIC VIRUS; TRITICUM;

EID: 38449083359     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(07)29003-2     Document Type: Chapter

References (31)

1. Browning K.S., Maia D.M., Lax S.R., and Ravel J.M. Identification of a new protein synthesis initiation factor from wheat germ. J. Biol. Chem. 262 (1987) 538-541
2. Browning K.S., Webster C., Roberts J.K., and Ravel J.M. Identification of an isozyme form of protein synthesis initiation factor 4F in plants. J. Biol. Chem. 267 (1992) 10096-10100
3. Bushell M., Wood W., Clemens M.J., and Morley S.J. Disruption of the interaction of mammalian protein synthesis eukaryotic initiation factor 4B with the poly(A)-binding protein by caspase- and viral protease-mediated cleavages. Eur. J. Biochem. 267 (2001) 1083-1091
4. Carrington J.C., and Freed D.D. Cap-independent enhancement of translation by a plant potyvirus 5′ nontranslated region. J. Virol. 64 (1990) 1590-1597
5. Gallie D.R. Cap-independent translation conferred by the 5′-leader of tobacco etch virus is eIF4G-dependent. J. Virol. 75 (2001) 12141-12152
6. Gallie D.R. Protein-protein interactions required during translation. Plant Mol. Biol. 50 (2002) 949-970
7. Gallie D.R. The 5′-leader of tobacco mosaic virus promotes translation through enhanced recruitment of eIF4F. Nucl. Acids Res. 30 (2002) 3401-3411
8. Gallie D.R., and Browning K.S. eIF4G functionally differs from eIFiso4G in promoting internal initiation, cap-independent translation, and translation of structured mRNAs. J. Biol. Chem. 276 (2001) 36951-36960
9. Gallie D.R., Feder J.N., Schimke R.T., and Walbot V. Post-transcriptional regulation in higher eukaryotes: The role of the reporter gene in controlling expression. Mol. Gen. Genet. 228 (1991) 258-264
10. Gallie D.R., Lucas W.J., and Walbot V. Visualizing mRNA expression in plant protoplasts: Factors influencing efficient mRNA uptake and translation. Plant Cell 1 (1989) 301-311
11. Gallie D.R., and Walbot V. RNA pseudoknot domain of tobacco mosaic virus can functionally substitute for a poly(A) tail in plant and animal cells. Genes Dev. 4 (1990) 1149-1157
12. Gallie D.R., and Walbot V. Identification of the motifs within the tobacco mosaic virus 5′ leader responsible for enhancing translation. Nucl. Acids Res. 20 (1992) 4631-4638
13. Gallie D.R., Ling J., Niepel M., Morley S.J., and Pain V.M. The role of 5′-leader length, secondary structure and PABP concentration on cap and poly(A) tail function during translation in Xenopus oocytes. Nucl. Acids Res. 28 (2000) 2943-2953
14. Gallie D.R., Sleat D.E., Watts J.W., Turner P.C., and Wilson T.M.A. The 5′-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo. Nucl. Acids Res. 15 (1987) 3257-3273
15. Gallie D.R., Sleat D.E., Watts J.W., Turner P.C., and Wilson T.M.A. Mutational analysis of the tobacco mosaic virus 5′-leader for altered ability to enhance translation. Nucl. Acids Res. 16 (1988) 883-893
16. Gallie D.R., Tanguay R.L., and Leathers V. The tobacco etch viral 5′ leader and poly(A) tail are synergistic regulators of translation. Gene 165 (1995) 233-238
17. Goyer C., Altmann M., Lee H.S., Blanc A., Deshmukh M., Woolford J.L., Trachsel H., and Sonenberg N. Tif4631 and Tif4632-Two yeast genes encoding the high-molecular-weight subunits of the cap-binding protein complex (eukaryotic initiation factor-4F) contain an RNA recognition motif-like sequence and carry out an essential function. Mol. Cell. Biol. 13 (1993) 4860-4874
18. Gradi A., Imataka H., Svitkin Y.V., Rom E., Raught B., Morino S., and Sonenberg N. A novel functional human eukaryotic translation initiation factor 4G. Mol. Cell. Biol. 18 (1998) 334-342
19. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
20. Lax S.R., Lauer S.J., Browning K.S., and Ravel J.M. Purification and properties of protein synthesis initiation and elongation factors from wheat germ. Methods Enzymol. 118 (1986) 109-128
21. Le H., Tanguay R.L., Balasta M.L., Wei C.-C., Browning K.S., Metz A.M., Goss D.J., and Gallie D.R. Translation initiation factors eIF-iso4G and eIF-4B interact with the poly(A)-binding protein and increase its RNA binding activity. J. Biol. Chem. 272 (1997) 16247-16255
22. Le H., Browning K.S., and Gallie D.R. The phosphorylation state of poly(A)-binding protein specifies its binding to poly(A) RNA and its interaction with eukaryotic initiation factor (eIF) 4F, eIFiso4F, and eIF4B. J. Biol. Chem. 275 (2000) 17452-17462
23. Leathers V., Tanguay R., Kobayashi M., and Gallie D.R. A phylogenetically conserved sequence within viral 3′ untranslated RNA pseudoknots regulates translation. Mol. Cell. Biol. 13 (1993) 5331-5347
24. Mundry K.W., Watkins P.A., Ashfield T., Plaskitt K.A., Eisele-Walter S., and Wilson T.M. Complete uncoating of the 5′ leader sequence of tobacco mosaic virus RNA occurs rapidly and is required to initiate cotranslational virus disassembly in vitro. J. Gen. Virol. 72 (1991) 769-777
25. Niepel M., and Gallie D.R. Identification and characterization of the functional elements within the tobacco etch viral 5′-leader required for cap-independent translation. J. Virol. 73 (1999) 9080-9088
26. Sambrook J., Fritsch E.F., and Maniatis T. "Molecular Cloning." (1989), Cold Spring Harbor Press, Cold Spring Harbor, NY
27. van Heerden A., and Browning K.S. Expression in Escherichia coli of the two subunits of the isozyme form of wheat germ protein synthesis initiation factor 4F. Purification of the subunits and formation of an enzymatically active complex. J. Biol. Chem. 269 (1994) 17454-17457
28. Wei CC., Balasta M.L., Ren J., and Goss D.J. Wheat germ poly(A) binding protein enhances the binding affinity of eukaryotic initiation factor 4F and (iso)4F for cap analogues. Biochemistry 37 (1998) 1910-1916
29. Wells D.R., Tanguay R.L., Le H., and Gallie D.R. HSP101 functions as a specific translational regulatory protein whose activity is regulated by nutrient status. Genes Devel. 12 (1998) 3236-3251
30. Wu X., Xu Z., and Shaw J.G. Uncoating of tobacco mosaic virus RNA in protoplasts. Virology 200 (1994) 256-262
31. Yisraeli J.K., and Melton D.A. Synthesis of long, capped transcripts in vitro by SP6 and T7 RNA polymerases. Methods Enzymol. 180 (1989) 42-50