Genetic transformation of Neurospora tetrasperma, demonstration of repeat-induced point mutation (RIP) in self-crosses and a screen for recessive RIP-defective mutants

Genetics

Volumn 167, Issue 3, 2004, Pages 1155-1164

  Bhat, Ashwin ^a   Tamuli, Ranjan ^a   Kasbekar, Durgadas P ^a  

^a CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; DNA; HYGROMYCIN; OXIDOREDUCTASE; STEROL;

ANTIBIOTIC RESISTANCE; ARTICLE; DNA SEQUENCE; ERG3 GENE; FUNGUS GROWTH; FUNGUS MUTANT; GENE; GENE MUTATION; GENE SILENCING; GENETIC CROSS; GENETIC TRANSFORMATION; GENOME; HOST RESISTANCE; MEIOSIS; NEUROSPORA; NEUROSPORA TETRASPERMA; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPEAT INDUCED POINT MUTATION; STRAIN DIFFERENCE; ULTRAVIOLET IRRADIATION;

BASE SEQUENCE; CINNAMATES; CROSSES, GENETIC; CULTURE MEDIA; DNA PRIMERS; GENES, FUNGAL; HYGROMYCIN B; MOLECULAR SEQUENCE DATA; MUTAGENESIS; NEUROSPORA; OXIDOREDUCTASES; POINT MUTATION; SEQUENCE ANALYSIS, DNA; TRANSFORMATION, GENETIC; TRANSGENES;

BACTERIA (MICROORGANISMS); FUNGI; NEUROSPORA; NEUROSPORA CRASSA; NEUROSPORA TETRASPERMA;

EID: 3843144002     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: 10.1534/genetics.103.025171     Document Type: Article

References (29)

1. BHAT, A., and D. P. KASBEKAR, 2001 Escape from repeat-induced point mutation of a gene-sized duplication in Neurospora crassa crosses that are heterozygous for a larger chromosome segment duplication. Genetics 157: 1581-1590.
2. BHAT, A., F. K. NOUBISSI, M. VYAS and D. P. KASBEKAR, 2003 Genetic analysis of wild-isolated Neurospora crassa strains identified as dominant suppressors of repeat-induced point mutation. Genetics 164: 947-961.
3. BISTIS, G. N., 1996 Trichogynes and fertilization in uni- and bimating type colonies of Neurospora tetrasperma. Fungal Genet. Biol. 20: 93-98.
4. CAMBARERI, E. B., B. C. JENSEN, E. SCHABTACH and E. U. SELKER, 1989 Repeat-induced G-C to A-T mutations in Neurospora. Science 244: 1571-1575.
5. DAVIS, R. H., and F. J. DE SERRES, 1970 Genetic and microbiological research techniques in Neurospora crassa. Methods Enzymol. A17: 79-143.
6. DODGE, B. O., 1927 Nuclear phenomena associated with heterothallism and homothallism in the ascomycete Neurospora. J. Agric. Res. 35: 289-305.
7. FREITAG, M., R. L. WILLIAMS, G. O. KOTHE and E. U. SELKER, 2002 A cytosine methyltransferase homologue is essential for repeat-induced point mutation in Neurospora crassa. Proc. Natl. Acad. Sci. USA 99: 8802-8807.
8. GALLEGOS, A., D. J. JACOBSON, N. R. RAJU, M. P. SKUPSKI and D. O. NATVIG, 2000 Suppressed recombination and a pairing anomaly on the mating type chromosome of Neurospora tetrasperma. Genetics 154: 623-633.
9. GRAIA, F., O. LESPINET, B. RIMBAULT, M. DEQUARD-CHABLAT, E. COPPIN et al., 2001 Genome quality control: RIP (repeat-induced point mutation) comes to Podospora. Mol. Microbiol. 40: 586-595.
10. HAMANN, A., F. FELLER and H. D. OSIEWACZ, 2000 The degenerate DNA transposon Pat and repeat-induced point mutation (RIP) in Podospora anserina. Mol. Gen. Genet. 263: 1061-1069.
11. MARGOLIN, B. S., M. FREITAG and E. U. SELKER, 1997 Improved plasmids for gene targeting at the his-3 locus of Neurospora crassa by electroporation. Fungal Genet. Newsl. 44: 34-36.
12. MERINO, S. T., M. A. NELSON, D. J. JACOBSON and D. O. NATVIG, 1996 Pseudohomothallism and evolution of the mating type chromosome in Neurospora tetrasperma. Genetics 143: 789-799.
13. METZENBERG, R. L., and S. K. AHLGREN, 1969 Hybrid strains useful in transferring genes from one species of Neurospora to another. Neurospora Newsl. 15: 9-10.
14. NOUBISSI, F. K., K. MCCLUSKEY and D. P. KASBEKAR, 2000 Repeat-induced point mutation (RIP) in crosses with wild-isolated strains of Neurospora crassa: evidence for dominant reduction of RIP. Fungal Genet. Biol. 31: 91-97.
15. PAGE, N., M. GERARD-VINCENT, P. MENARD, M. BEAULIEU, M. AZUMA et al., 2003 A Saccharomyces cerevisiae genome-wide mutant screen for altered sensitivity to K1 killer toxin. Genetics 163: 875-894.
16. PAPAVINASASUNDARAM, K. G., and D. P. KASBEKAR, 1994 The Neurospora crassa erg-3 gene encodes a protein with sequence homology to both yeast sterol C-14 reductase and chicken lamin B receptor. J. Genet. 73: 33-41.
17. PERKINS, D. D., 1991 Transfer of genes and translocations from Neurospora crassa to N. tetrasperma. Fungal Genet. Newsl. 38: 84.
18. PERKINS, D. D., A. RADFORD and M. S. SACHS, 2001 The Neurospora Compendium: Chromosomal Loci. Academic Press, San Diego.
19. PRAKASH, A., and D. P. KASBEKAR, 2002a The sterol C-14 reductase encoded by the Neurospora crassa erg-3 gene: essential charged and polar residues identified by site-specific mutagenesis. Mol. Genet. Genomics 266: 787-795.
20. PRAKASH, A., and D. P. KASBEKAR, 2002b Genes encoding chimeras of Neurospora crassa erg-3 and human TM7SF2 proteins fail to complement Neurospora and yeast sterol C-14 reductase mutants. J. Biosci. 27: 105-112.
21. PRAKASH, A., S. SENGUPTA, K, APARNA and D. P. KASBEKAR, 1999 The erg-3 (sterol delta14,15-reductase) gene of Neurospora crassa: generation of null mutants by repeat-induced point mutation and complementation by proteins chimeric for human lamin B receptor sequences. Microbiology 145: 1443-1451.
22. RAJU, N. B., 1992 Functional heterothallism resulting from homokaryotic conidia and ascospores in Neurospora tetrasperma. Mycol. Res. 96: 103-116.
23. RAJU, N. B., and D. D. PERKINS, 1994 Diverse programs of ascus development in pseudohomothallic species of Neurospora, Gelasinospora and Podospora. Dev. Genet. 15: 104-118.
24. SELKER, E. U., 1990 Premeiotic instability of repeated sequences in Neurospora crassa. Annu. Rev. Genet. 24: 597-613.
25. SENGUPTA, S., T. B. PRASANNA and D. P. KASBEKAR, 1995 Sterol 14, 15 reductase (erg-3) mutants switch the phenotype of Neurospora crassa from sensitivity to the tomato saponin α-tomatine to sensitivity to the pea phyoalexin pisatin. Fungal Genet. Newsl. 42: 71-72.
26. SHIU, P. K. T., and R. L. METZENBERG, 2002 Meiotic silencing by unpaired DNA: properties, regulation and suppression. Genetics 161: 1483-1495.
27. SHIU, P. K. T., N. B. RAJU, D. ZICKLER and R. L. METZENBERG, 2001 Meiotic silencing by unpaired DNA. Cell 107: 905-916.
28. SINGER, M. J., E. A. KOZMINOVA, A. THARP, B. S. MARGOLIN and E. U. SELKER, 1995 Different frequencies of RIP among early vs. late ascospores of Neurospora crassa. Fungal Genet. Newsl. 42: 74-75.
29. STABEN, C., P. JENSEN, J. SINGER, J. POLLOCK, M. SCHECTMAN et al., 1989 Use of bacterial hygromycin B resistance gene as a dominant selectable marker in Neurospora crassa transformation. Fungal Genet. Newsl. 36: 79-81.