The erg-3 (sterol Δ14,15-reductase) gene of Neurospora crassa: Generation of null mutants by repeat-induced point mutation and complementation by proteins chimeric for human lamin B receptor sequences

Microbiology

Volumn 145, Issue 6, 1999, Pages 1443-1451

  Prakash, A ^a   Sengupta, Saswati ^a   Aparna, K ^a   Kasbekar, Durgadas P ^a  

^a CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY   (India)

Author keywords

Biochanin A; Isoflavonoids; Lamin B receptor; Pisatin; Tomatine

Indexed keywords

BIOCHANIN A; LAMIN B; PHYTOALEXIN;

AMINO ACID SEQUENCE; ARTICLE; FUNGAL GENETICS; FUNGUS MUTATION; GENE SILENCING; GENETIC COMPLEMENTATION; GENETIC TRANSFORMATION; NEUROSPORA CRASSA; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL; RECOMBINANT GENE;

FUNGI; NEUROSPORA CRASSA;

EID: 0033019343     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/13500872-145-6-1443     Document Type: Article

References (35)

1. Akins, R. A. & Lambowitz, A. M. (1985). General method for cloning Neurospora crassa nuclear genes by complementation of mutants. Mol Cell Biol 5, 2272-2278.
2. Aparna, K., Sandrock, R. W. & Kasbekar, D. P. (1998). Cloning of the sterol C-14 reductase gene of the tomato pathogenic fungus Septoria lycopersici and its complementation of the erg-3 mutation of Neurospora crassa. J Genet 77, 71-75.
3. Baloch, R. I., Mercer, E. I., Wiggins, T. E. & Baldwin, B. C. (1984). Inhibition of ergosterol biosynthesis in Saccharomyces cerevisiae and Ustilago maydis by tridemorph, fenpropimorph and fenpropidin. Phytochemistry 23, 2219-2226.
4. Crowley, J. H., Smith, S. J., Leak, F. W. & Parks, L. W. (1996). Aerobic isolation of an ERG24 null mutant of Saccharomyces cerevisiae. J Bacteriol 178, 2991-2993.
5. Davis, R. H. & De Serres, J. F. (1970). Genetic and microbiological research techniques for Neurospora crassa. Methods Enzymol A17, 79-143.
6. Defago, G. & Kern, H. (1983). Induction of Fusarium salani mutants insensitive to tomatine, their pathogenicity and aggressiveness to tomato fruits and pea plants. Physiol Plant Pathol 22, 29-37.
7. Defago, G., Kern, H. & Sedlar, L. (1983). Genetic analysis of tomatine insensitivity, sterol content and pathogenicity for green tomato fruits in mutants of Fusarium solani. Physiol Plant Pathol 22, 39-43.
8. 14,15-reductase activity. J Gen Microbiol 137, 2627-2630.
9. Foss, E. J., Garret P. W., Kinsey, J. A. & Selker, E. U. (1991). Specificity of repeat induced point mutation (RIP) in Neurospora: sensitivity of non-Neurospora sequences, a natural diverged tandem duplication, and unique DNA adjacent to a duplicated region. Genetics 127, 711-717.
10. Grindle, M. (1973). Sterol mutants of Neurospora crassa: their isolation, growth characteristics and resistance to polyene antibiotics. Mol Gen Genet 120, 283-290.
11. Grindle, M. (1974). The efficacy of various mutagens and polyene antibiotics for the induction and isolation of sterol mutants of Neurospora crassa. Mol Gen Genet 130, 81-90.
12. Grotelueschen, J. & Metzenberg, R. W. (1995). Some property of the nucleus determines the competence of Neurospora crassa for transformation. Genetics 139, 1545-1551.
13. Holmer, L., Pezhman, A. & Worman, H. J. (1998). The human lamin B receptor/sterol reductase multigene family. Genomics 54, 469-476.
14. Irelan, J. T. & Selker, E. U. (1996). Gene silencing in filamentous fungi: RIP, MIP and quelling. J Genet 75, 313-324.
15. Irelan, J. T. & Selker, E. U. (1997). Cytosine methylation associated with repeat-induced point mutation causes epigenetic gene silencing in Neurospora crassa. Genetics 146, 509-523.
16. Lai, M. H., Bard, M., Pierson, C. A., Alexander, J. F., Goebl, M., Carter, G. T. & Kirsch, D. R. (1994). The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway. Gene 140, 41-49.
17. Lorenz, R. T. & Parks, L. W. (1992). Cloning, sequencing and disruption of the gene encoding sterol C-14 reductase in Saccharomyces cerevisiae. DNA Cell Biol 11, 685-692.
18. Marcireau, C., Guyonnet, D. & Karst, F. (1992). Construction and growth properties of a yeast strain defective in sterol 14-reductase. Curr Genet 22, 267-272.
19. Miao, V. P. W., Rountree, M. R. & Selker, E. U. (1995). Ectopic integration of transforming DNA is rare among Neurospora transformants selected for gene replacement. Genetics 139, 1533-1544.
20. cdc2. J Biol Chem 272, 6208-6213.
21. Pandit, N. N. & Russo, V. E. A. (1992). Reversible inactivation of a foreign gene, hph, during the asexual cycle in Neurospora crassa transformants. Mol Gen Genet 234, 412-422.
22. Papavinasasundaram, K. G. & Kasbekar, D.P. (1993). Pisatin resistance in Dictyostelium discoideum and Neurospora crassa: comparison of mutant phenotypes. J Gen Microbiol 139, 3035-3041.
23. Papavinasasundaram, K. G. & Kasbekar, D. P. (1994). The Neurospora crassa erg3 gene encodes a protein with sequence homology to both yeast sterol C-14 reductase and chicken lamin B receptor. J Genet 73, 33-41.
24. Perkins, D. D., Radford, A., Newmeyer, D. & Bjorkman, M. (1982). Chromosomal loci of Neurospora crassa. Microbiol Rev 46, 426-570.
25. Schuler, E., Lin, F. & Worman, H. J. (1994). Characterization of the human gene encoding LBR, an integral protein of the nuclear envelope inner membrane. J Biol Chem 269, 11312-11317.
26. Selker, E. U. (1990). Premeiotic instability of repeated sequences in Neurospora crassa. Annu Rev Genet 24, 597-613.
27. Sengupta, S., Bhavani Prasanna, T. & Kasbekar, D. P. (1995). Sterol 14,15 reductase (erg-3) mutants switch the phenotype of Neurospora crassa from sensitivity to the tomato saponin α-tomatine to sensitivity to the pea phytoalexin pisatin. Fungal Genet Newsl 42, 71-72.
28. Silve, S., Dupuy, P.-H., Ferrara, P. & Loison, G. (1998). Human lamin B receptor exhibits sterol C14-reductase activity in Saccharomyces cerevisiae. Biochim Biophys Acta 1392, 233-244.
29. Staben, C., Jensen, B., Singer, J., Pollock, J., Schectman, M., Kinsey, J. & Selker, E. (1989). Use of bacterial hygromycin B resistance gene as a dominant selectable marker in Neurospora crassa transformation. Fungal Genet Newsl 36, 79-81.
30. Sweigard, J. & VanEtten, H. D. (1987). Reduction in pisatin sensitivity of Aphanomyces euteiches by polar lipid extracts. Phytopathology 77, 771-775.
31. Vollmer, S. J. & Yanofsky, C. (1986). Efficient cloning of genes of Neurospora crassa. Proc Natl Acad Sci USA 34, 573-577.
32. Watters, M. K. & Stadler, D. R. (1995). Spontaneous mutation during the sexual cycle of Neurospora crassa. Genetics 139, 137-145.
33. Worman, H. J., Evans, C. D. & Blobel, G. (1990). The lamin B receptor of the nuclear envelope inner membrane: a polytopic protein with eight potential transmembrane domains. J Cell Biol 111, 1535-1542.
34. Ye, Q. & Worman, H. J. (1994). Primary structure analysis and lamin B and DNA binding of human LBR, an integral protein of the nuclear envelope inner membrane. J Biol Chem 269, 11306-11311.
35. Ye, Q. & Worman, H. J. (1996). Interaction between an integral protein of the nuclear envelope inner membrane and human chromodomain proteins homologous to Drosophila HP1. J Biol Chem 271, 14653-14656.