Kinetic analysis of tRNA-directed transcription antitermination of the Bacillus subtilis glyQS gene in vitro

Journal of Bacteriology

Volumn 186, Issue 16, 2004, Pages 5392-5399

  Grundy, Frank J ^a   Henkin, Tina M ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELONGATION FACTOR; ELONGATION FACTOR NUSA; ELONGATION FACTOR NUSG; GLYCINE; SIGNAL PEPTIDE; TRANSFER RNA; UNCLASSIFIED DRUG;

ANTICODON; ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; BASE PAIRING; BIOASSAY; GLYQS GENE; IN VITRO STUDY; KINETICS; LEADER REGION TRANSCRIPTION TERMINATION SIGNAL; MODULATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN RNA BINDING; SIGNAL PROCESSING; TATA BOX; TRANSCRIPTION ANTITERMINATION; TRANSCRIPTION PAUSE SITE; TRANSCRIPTION PAUSING; TRANSCRIPTION TERMINATION;

5' UNTRANSLATED REGIONS; ANTICODON; BACILLUS SUBTILIS; BASE PAIRING; BASE SEQUENCE; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PEPTIDE ELONGATION FACTORS; REGULATORY SEQUENCES, NUCLEIC ACID; RNA, BACTERIAL; RNA, MESSENGER; RNA, TRANSFER, GLY; RNA-BINDING PROTEINS; TERMINATOR REGIONS (GENETICS); TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); POSIBACTERIA; PROKARYOTA;

EID: 3843118724     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.186.16.5392-5399.2004     Document Type: Article

References (25)

1. Artsimovitch, I. Control of transcription termination and antitermination. In N. P. Higgins (ed.), The bacterial chromosome, in press. ASM Press, Washington, D.C.
2. Artsimovitch, I., and R. Landick. 2000. Pausing by bacterial RNA polymerase is mediated by mechanistically distinct classes of signals. Proc. Natl. Acad. Sci. USA 97:7090-7095.
3. Artsimovitch, I., V. Svetlov, L. Anthony, R. R. Burgess, and R. Landick. 2000. RNA polymerases from Bacillus subtilis and Escherichia coli differ in recognition of regulatory signals in vitro. J. Bacteriol. 182:6027-6035.
4. d'Aubenton Carafa, Y., E. Brody, and C. Thermes. 1990. Prediction of Rho-independent Escherichia coli transcription terminators. A statistical analysis of their stem-loop structures. J. Mol. Biol. 216:835-858.
5. Gong, F., and C. Yanofsky. 2003. A transcriptional pause synchronizes translation with transcription in the tryptophanase operon leader region. J. Bacteriol. 185:6472-6476.
6. Grundy, F. J., and T. M. Henkin. 1993. tRNA as a positive regulator of transcription antitermination in B. subtilis. Cell 74:475-482.
7. Grundy, F. J., and T. M. Henkin. 2003. The T box and S box transcription termination control systems. Front. Biosci. 8:d20-d31.
8. Grundy, F. J., S. E. Hodil, S. M. Rollins, and T. M. Henkin. 1997. Specificity of tRNA-mRNA interactions in Bacillus subtilis tyrS antitermination. J. Bacteriol. 179:2587-2594.
9. Grundy, F. J., T. R. Moir, M. T. Haldeman, and T. M. Henkin. 2002. Sequence requirements for terminators and antiterminators in the T box transcription antitermination system: disparity between conservation and functional requirements. Nucleic Acids Res. 30:1646-1655.
10. Grundy, F. J., S. M. Rollins, and T. M. Henkin. 1994. Interaction between the acceptor end of tRNA and the T box stimulates antitermination in the Bacillus subtilis tyrS gene: a new role for the discriminator base. J. Bacteriol. 235:798-804.
11. Grundy, F. J., W. C. Winkler, and T. M. Henkin. 2002. tRNA-mediated transcription antitermination in vitro: codon-anticodon pairing independent of the ribosome. Proc. Natl. Acad. Sci. USA 99:11121-11126.
12. Hager, D. A., D. J. Jin, and R. R. Burgess. 1990. Use of Mono Q high-resolution ion-exchange chromatography to obtain highly pure and active Escherichia coli RNA polymerase. Biochemistry 29:7890-7894.
13. Henkin, T. M., and C. Yanofsky. 2002. Regulation by transcription attenuation in bacteria: how RNA provides instructions for transcription termination/antitermination decisions. Bioessays 24:700-707.
14. Landick, R., J. Carey, and C. Yanofsky. 1985. Translation, activates the paused transcription complex and restores transcription of the trp operon leader region. Proc. Natl. Acad. Sci. USA 82:4663-4667.
15. Landick, R., D. Wang, and C. L. Chan. 1996. Quantitative analysis of transcriptional pausing by Escherichia coli RNA polymerase: his leader pause site as paradigm. Methods Enzymol. 274:334-353.
16. McDowell, J. C., J. W. Roberts, D. J. Jin, and C. Gross. 1994. Determination of intrinsic transcription termination efficiency by RNA polymerase elongation rate. Science 266:822-825.
17. Mosteller, R. D., and C. Yanofsky. 1970. Transcription of the tryptophan operon in Escherichia coli: rifampicin as an inhibitor of initiation. J. Mol. Biol. 48:525-531.
18. Putzer, H., C. Condon, D. Brechemier-Baey, R. Brito, and M. Grunberg-Manago. 2002. Transfer RNA-mediated antitermination in vitro. Nucleic Acids Res. 30:3026-3033.
19. A holoenzyme are sufficient for transcription of Pho regulon promoters in Bacillus subtilis: PhoP-P activator sites within the coding region stimulate transcription in vitro. Mol. Microbiol. 28:1187-1197.
20. Rollins, S. M., F. J. Grundy, and T. M. Henkin. 1997. Analysis of cis-acting sequence and structural elements required for antitermination of the Bacillus subtilis tyrS gene. Mol. Microbiol. 25:411-421.
21. Schmidt, M. C., and M. J. Chamberlin. 1987. nusA protein of Escherichia coli is an efficient transcription termination factor for certain termination sites. J. Mol. Biol. 195:809-818.
22. Sha, Y., L. Lindahl, and J. M. Zengel. 1995. Role of NusA in L4-mediated attenuation control of the S10 r-protein operon of Escherichia coli. J. Mol. Biol. 245:474-485.
23. Vogel, U., and K. F. Jensen. 1994. The RNA chain elongation rate in Escherichia coli depends on the growth rate. J. Bacteriol. 176:2807-2813.
24. Yakhnin, A. V., and P. Babitzke. 2002. NusA-stimulated RNA polymerase pausing and termination participates in the Bacillus subtilis trp operon attenuation mechanism in vitro. Proc. Natl. Acad. Sci. USA 99:11067-11072.
25. Zhang, H., and R. L. Switzer. 2003. Transcriptional pausing in the Bacillus subtilis pyr operon in vitro: a role in transcriptional attenuation? J. Bacteriol. 185:4764-4771.