A new and simple method for rapid extraction and isolation of high-quality RNA from grape (Vitis vinifera) berries

Journal of the Science of Food and Agriculture

Volumn 88, Issue 2, 2008, Pages 179-184

  Fort, Francesca ^a   Hayoun, Loubna ^a   Valls, Josep ^a   Canals, Joan M ^a   Arola, Lluís ^a   Zamora, Fernando ^a  

^a UNIVERSITAT ROVIRA I VIRGILI   (Spain)

Author keywords

Extraction; Grape berries; Isolation; RNA; Vitis vinifera

Indexed keywords

VITACEAE; VITIS VINIFERA;

EID: 38349100796     PISSN: 00225142     EISSN: 10970010     Source Type: Journal    
DOI: 10.1002/jsfa.3066     Document Type: Article

References (22)

1. Considine JA and Knox RB, Development and histochemistry of the cells, cell walls, and cuticle of the dermal system of fruit of the grape, Vitis vinifera L. Protoplasma 26:347-365 (1979).
2. Loulakakis KA, Roubelakis-Angelakis KA and Kanellis AK, Isolation of functional RNA from grapevine tissues poor in nucleic acid content. Am J Enol Vitic 47:181-185 (1996).
3. Loomis J, Overcoming problems of phenolics and quinines in the isolation of plant enzymes and organelles. Meth Enzymol 31:528-544 (1974).
4. Xu Q, Wen X and Deng X, A simple protocol for isolating genomic DNA from chestnut rose (Rosa roxburghii Tratt) for RFLP and PRC analyses. Plant Mol Biol Rep 22:301a-301g (2004).
5. Salzman RA, Fujita T, Zhu-Salzman K, Hasegawa PM and Bressan RA, An improved RNA isolation method for plant tissues containing high levels of phenolic compounds or carbohydrates. Plant Mol Biol Rep 17:11-17 (1999).
6. Murray MG and Thompson WF, Rapid isolation for high molecular weight DNA. Nucleic Acids Res 8:4321-4325 (1980).
7. Merlo DJ and Kemp JD, Effect of polysaccharides on kinetics of DNA. Plant Physiol 58:1522-1526 (1976).
8. Do N and Adams RP, A simple technique for removing plant polysaccharide contaminant from DNA. Biotechniques 10:162-166 (1991).
9. Porebski S, Bailey LG and Baum BR, Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep 15:8-15 (1997).
10. Manning K, Isolation of nucleic acids from plants by differential solvent precipitation. Anal Biochem 195:45-50 (1991).
11. Tesniere C and Vayda ME, Method for the isolation of high-quality RNA from grape berry tissues without contaminating tannins or carbohydrates. Plant Mol Biol Rep 9:242-251 (1991).
12. Franke KE, Liu Y and Adams DO, Yield and quality of RNA from grape berries at different developmental stages. Am J Enol Vitic 46:315-318 (1995).
13. Baiges I and Mas A, Vitis RNA extraction method adapted from a DNA isolation protocol. J Int Sci Vigne Vin 37:59-61 (2003).
14. Moser C, Gatto P, Moser M, Pindo M and Velasco R, Isolation of functional RNA from small amounts of different grape and apple tissues. Mol Biotechnol 26:95-99 (2004).
15. Iandolino AB, Goes da Silva F, Lim H, Choi H, Williams LE and Cook DR, High-quality RNA, cDNA, and derived EST libraries from grapevine (Vitis vinifera L.). Plant Mol Biol Rep 22:269-278 (2004).
16. Del Aguila EM, Dutra MB, Silva JT and Paschoalin VMF, Comparing protocols for preparation of DNA-free total yeast RNA suitable for RT-PCR. BMC Mol Biol 6:9-14 (2005).
17. Maniaris T, Fritsch EF and Sambrook J, Molecular Cloning. A Laboratory Manual, ed. by Cold Spring Harbor Laboratory, Cold Springs Harbor, New York, 2nd ed., Vol 3. pp E.3-E. 12 (1989).
18. Tattersall EAR, Ergul A, AlKayal F, DeLuc L, Cushman JC and Cramer GR, Comparison of methods for isolating high-quality RNA from leaves of grapevine. Am J Enol Vitic 56:400-406 (2005).
19. Surzycki S, General aspects of RNA isolation and purification, in Basic Techniques in Molecular Biology, ed. by Stefan Surzycki, Springer-Verlag, Berlin, Heidelberg, New York, pp. 119-144 (2000).
20. Steenkamp J, Wild I, Lourens A and van Helden P, Improved method for DNA extraction from Vitis vinifera. Am J Enol Vitic 45:102-104 (1994).
21. King EE, Extraction of cotton leaf enzymes with borate. Phytochemistry 10:2337-2341 (1971).
22. Gauch HG and Dugger WM, The role of boron in the translocation of sucrose. Plant Physiol 28:457-466 (1953).