Ziehl-Neelsen staining: Theory and practice

International Journal of Tuberculosis and Lung Disease

Volumn 12, Issue 1, 2008, Pages 108-110

  Van Deun, Armand ^a,b   Hossain, M A ^c   Gumusboga, M ^a   Rieder, H L ^b  

^a INSTITUTE OF TROPICAL MEDICINE   (Belgium)

^b INTERNATIONAL UNION AGAINST TUBERCULOSIS AND LUNG DISEASE   (France)

^c Damien Foundation Bangladesh   (Bangladesh)

Author keywords

Basic fuchsin; Microscopy; Staining; Ziehl Neelsen

Indexed keywords

FUCHSINE;

ARTICLE; EXPERIMENTAL STUDY; MEDICAL INFORMATION; PRACTICE GUIDELINE; PRIORITY JOURNAL; STAINING; THEORY-PRACTICE RELATIONSHIP; TUBERCULOSIS; WORLD HEALTH ORGANIZATION;

BACTERIOLOGICAL TECHNIQUES; COLORING AGENTS; HUMANS; MYCOBACTERIUM TUBERCULOSIS; PRACTICE GUIDELINES AS TOPIC; QUALITY CONTROL; ROSANILINE DYES; STAINING AND LABELING; TIME FACTORS; TUBERCULOSIS; WORLD HEALTH ORGANIZATION;

EID: 38349041626     PISSN: 10273719     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (8)

1. World Health Organization. Laboratory services in tuberculosis control. Part II: microscopy. WHO/TB/98.258. Geneva, Switzerland: WHO, 1998.
2. International Union Against Tuberculosis and Lung Disease. Technical guide. Sputum examination for tuberculosis by direct microscopy in low income countries. Paris, France: IUATLD, 2000.
3. Harada K, Gidoh S, Tsutsumi S. Staining mycobacteria with carbolfuchsin: properties of solutions with different samples of basic fuchsin. Microscopica Acta 1976; 78: 21-27.
4. Yegian D, Budd V. Ziehl-Neelsen technique. Staining properties by different preparations of basic fuchsin. Am Rev Tuberc 1943; 48: 54-57.
5. Rieder H L, Van Deun A, Kam K M, et al. Priorities for tuberculosis bacteriology services in low-income countries. 2nd ed. Paris, France: International Union Against Tuberculosis and Lung Disease, 2007.
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7. Van Deun A, Roorda F A, Chambuganj N, Hye M A, Hossain M. Reproducibility of sputum smear examination for acid-fast bacilli: practical problems met during cross-checking. Int J Tuberc Lung Dis 1999; 3: 823-829.
8. Selvakumar N, Prabhakaran E, Rehman F, Frieden T R, Santha T. Washing of new microscopic glass slides in dichromate solution does not influence sputum AFB smear results. Int J Tuberc Lung Dis 2002; 6: 270-272.