Performance of variations of carbolfuchsin staining of sputum smears for AFB under field conditions

International Journal of Tuberculosis and Lung Disease

Volumn 9, Issue 10, 2005, Pages 1127-1133

  Van Deun, Armand ^a,b   Salim, A Hamid ^c   Aung, K J M ^c   Hossain, Md A ^c   Chambugonj, N ^c   Hye, Md A ^c   Kawria, A ^c   Declercq, E ^d  

^a INSTITUTE OF TROPICAL MEDICINE   (Belgium)

^b INTERNATIONAL UNION AGAINST TUBERCULOSIS AND LUNG DISEASE   (France)

^c Damien Foundation Bangladesh   (Bangladesh)

^d Damien Foundation   (Belgium)

Author keywords

Carbolfuchsin; Microscopy; Staining; Ziehl Neelsen

Indexed keywords

CARBOLFUCHSIN; FUCHSINE; UNCLASSIFIED DRUG;

ACID FAST BACTERIUM; ARTICLE; BANGLADESH; DIAGNOSTIC ERROR; HEALTH CARE ORGANIZATION; HEALTH PROGRAM; HUMAN; LUNG DISEASE; MAJOR CLINICAL STUDY; MEDICAL EXAMINATION; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SPUTUM SMEAR; STAINING; TUBERCULOSIS; WORLD HEALTH ORGANIZATION;

BANGLADESH; CHI-SQUARE DISTRIBUTION; COLORING AGENTS; HUMANS; MICROSCOPY; ROSANILINE DYES; SENSITIVITY AND SPECIFICITY; SPUTUM; STAINING AND LABELING; TUBERCULOSIS, PULMONARY;

EID: 26444546716     PISSN: 10273719     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. World Health Organization. Treatment of tuberculosis: guidelines for national programmes. 3rd ed. WHO/CDS/TB/2003.313. Geneva, Switzerland: WHO, 2003.
2. World Health Organization. Laboratory services in tuberculosis control. Part II: Microscopy. 1st ed. WHO/TB/98.258. Geneva, Switzerland: WHO, 1998.
3. International Union Against Tuberculosis and Lung Disease. Technical guide. Sputum examination for tuberculosis by direct microscopy in low income countries. 5th ed. Paris, France: IUATLD, 2000.
4. Bishop P J, Neumann G. The history of the Ziehl-Neelsen stain. Tubercle 1970; 51: 196-206.
5. Rieder H L, Chonde T M, Myking H, et al. The public health service national tuberculosis reference laboratory and the national laboratory network. Minimum requirements, role and operation in a low-income country. Paris, France: International Union Against Tuberculosis and Lung Disease, 1998.
6. Sticht-Groh V, Bretzel G. Staining for acid-fast bacilli: hot or cold? [Correspondence]. Tubercle Lung Dis 1998; 75: 318.
7. Buzingo T, Sanders M, Masabo J P, Nyandwi S, Van Deun A. Systematic restaining of sputum smears for quality control is useful in Burundi. Int J Tuberc Lung Dis 2003; 7: 439-444.
8. Kinyoun J J. A note on Uhlenhuths method for sputum examination, for tubercle bacilli. Am J Public Health 1915; 5: 867-870.
9. Tan Thiam Hok. A simple and rapid cold-staining method for acid-fast bacilli. [Correspondence]. Am Rev Respir Dis 1962; 85: 753-754.
10. Allen J L. A modified Ziehl-Neelsen stain for mycobacteria. Med Lab Sciences 1992; 49: 99-102.
11. Devulder B. Coloration des bacilles acido-alcoolo-résitants par la technique de Tan Thiam Hok modifiée. Ann Inst Pasteur Lille 1963; 14: 229-231.
12. Engbaek H C, Bennedsen J, Olesen Larsen S. Comparison of various staining methods for demonstration of tubercle bacilli in sputum by direct microscopy. Bull Int Union Tuberc 1969; 42: 94-110.
13. Slosarek M, Mezenský L. Cold staining methods for mycobacteria. J Hyg Epidemiol Microbiol Immunol 1974; 18: 22-28.
14. Noack K. Die Leistungsfähigkeit von Kaltfärbeverfahren beim lichtmikroskopischen Nachweis säurefester Bakterien. Z Erkrank Atm Org 1979; 153: 132-138.
15. Mathew S, Alexander C, Thyagarajan K, Krishnamurthy P V, Paramasivan C N. Evaluation of a cold staining method for acid-fast bacilli in sputum. Ind J Chest Dis All Sci 1994; 36: 125-131.
16. Pathan A J, Arain A R. Comparative study of cold stain and Ziehl-Neelsen stain. Tubercle 1988; 69: 227-229.
17. Kochhar A. Evaluation of a two step AFB cold staining method and simplified concentration technique for diagnosis of pulmonary tuberculosis. J Commun Dis 2002; 34: 276-280.
18. Selvakumar N, Gomathi M, Rehman F, Narayanan P R. Evaluation of a two-reagent cold staining method for detection of acid-fast bacilli. Int J Tuberc Lung Dis 2002; 6: 728-731.
19. Vasanthakumari R, Jagannath K, Rajasekaran S. A cold staining method for acid-fast bacilli. Bull World Health Organ 1986; 64: 741-743.
20. Gruft H. Evaluation of mycobacteriology laboratories: the acid-fast smear. Health Lab Sci 1978; 15: 215-219.
21. Somoskövi Á, Hotaling J E, Fitzgerald M, O'Donnell D, Parsons L M, Salfinger M. Lessons from a proficiency testing event for acid-fast microscopy. Chest 2001; 120: 250-257.
22. Yegian D, Budd V. Ziehl-Neelsen technique. Staining properties by different preparations of basic fuchsin. Am Rev Respir Dis 1943; 48: 54-57.
23. Darrow M A. Staining the tubercle organism in sputum smears. Stain Technol 1949; 24: 93-94.
24. Selvakumar N, Rahman F, Rajasekaran S, Narayanan P R, Frieden T R. Inefficiency of 0.3% carbol fuchsin in Ziehl-Neelsen staining for detecting acid-fast bacilli. J Clin Microbiol 2002; 40: 3041-3043.
25. Angra P, Ridderhof J, Smithwick R. Comparison of two different strengths of carbol fuchsin in Ziehl-Neelsen staining for detecting acid-fast bacilli. [Correspondence]. J Clin Microbiol 2003; 41: 3459.
26. Selvakumar N, Sekar M G, Rahman F, et al. Comparison of variants of carbol-fuchsin solution in Ziehl-Neelsen for detection of acid-fast bacilli. Int J Tuberc Lung Dis 2005; 9: 226-229.
27. Aziz M A, Ba F, Becx-Bleumink M, et al. External quality assessment for AFB smear microscopy. Washington, DC: Association of Public Health Laboratories, 2002.
28. International Union Against Tuberculosis. Technical guide for sputum examination for tuberculosis by direct microscopy. Bull Int Union Tuberc Lung Dis 1978; 53 (Suppl 2): 4-16.
29. Slosarek M, Dondov C, Dabrowska L, et al. Comparison of microscopic positivity in smears from sputa stained according to Ziehl-Neelsen in different modifications. J Hyg Epidemiol Microbiol Immunol 1977; 21: 7-15.