Quantitative detection of Monosporascus cannonballus in infected melon roots using real-time PCR

European Journal of Plant Pathology

Volumn 120, Issue 2, 2008, Pages 147-156

  Picó, Belén ^a,b   Roig, Cristina ^a   Fita, Ana ^a   Nuez, Fernando ^a  

^a Institute for the Conservation and Breeding of Agricultural Biodiversity (COMAV)   (Spain)

^b UNIVERSITAT POLITÈCNICA DE VALÈNCIA   (Spain)

Author keywords

Ascomycetes; Cucumis melo; Disease assessment; ITS regions; Root rot vine decline; SYBR Green

Indexed keywords

CULTIVAR; DETECTION METHOD; DISEASE SEVERITY; DYE; FRUIT; FUNGUS; GROWTH RATE; LESION; POLYMERASE CHAIN REACTION; ROOT ROT; SENSITIVITY ANALYSIS;

ASCOMYCOTA; CUCUMIS; CUCUMIS MELO; FUNGI; MONOSPORASCUS CANNONBALLUS;

EID: 38149139487     PISSN: 09291873     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10658-007-9203-z     Document Type: Article

References (20)

1. Atkins, S. D., & Clark, I. M. (2004). Fungal molecular diagnostics: a mini review. Journal of Applied Genetics, 45, 3-15.
2. Atkins, S. D., Clark, I. M., Hirsch, P. R., & Kerry, B. R. (2005). The use of real-time PCR and species-specific primers for the identification and monitoring of Paecilomyces lilacinus. FEMS Microbiology Ecology, 51, 257-264.
3. Bruton, B. D., García-Jimémez, J., Armengol, J., & Popham, T. W. (2000). Assessment of virulence of Acremonium cucurbitacearum and Monosporascus cannonballus on Cucumis melo. Plant Disease, 84, 907-913.
4. Cohen, R., Pivonia, S., Burger, Y., Edelstein, M., Gamliel, A., & Katan, J. (2000). Toward integrated management of Monosporascus wilt of melons in Israel. Plant Disease, 84, 496-505.
5. Collado, J., González, A., Platas, G., Stechiguel, A. M., Guarro, J., & Pelaez, F. (2002). Monosporascus ibericus sp. nov., an entophytic ascomycete from plants on saline soils, with observations on the position of the genus based on sequence analysis of the 18 S rDNA. Mycological Research, 106, 118-127.
6. Crosby, K., Wolff, D., & Miller, M. (2000). Comparisons of root morphology in susceptible and tolerant melon cultivars before and after infection by Monosporascus cannonballus. HortScience, 35, 681-683.
7. Cullen, D. W., Lees, A. K., Toth, I. K., & Duncan, J. K. (2001). Conventional PCR and real-time quantitative PCR detection of Helminthosporium solani in soil and on potato tubers. European Journal of Plant Pathology, 107, 387-398.
8. Dias, R. C., Picó, B., Espinós, A., & Nuez, F. (2004). Resistance to melon vine decline derived from C. melo subsp. agrestis: genetic analysis of root structure and root response to the disease. Plant Breeding, 123, 1-7.
9. Dias, R. C., Picó, B., Herraiz, J., Espinós, A., & Nuez, F. (2002). Modifying root structure on cultivated muskmelon to improve vine decline resistance. HortScience, 37, 1092-1097.
10. Filion, M., St-Arnaud, M., & Jabaji-Hare, S. H. (2003). Direct quantification of fungal DNA from soil substrate using real-time PCR. Journal of Microbiological Methods, 53, 67-76.
11. Fita, A., Picó, B., & Nuez, F. (2006). Análisis de imagen como herramienta en la mejora del sistema radicular de melón. Actas de Horticultura, 45, 119-120.
12. Gao, X., Jackson, T. A., Lambert, K. N., Li, S., Hartman, G. L., & Niblack, T. L. (2004). Detection and quantification of Fusarium solani f. sp. glycines in soyabean roots with quantitative polymerase chain reaction. Plant Disease, 88, 1372-1380.
13. Iglesias, A., Picó, B., & Nuez, F. (2000). A temporal genetic analysis of disease resistance genes: resistance to melon vine decline derived from C. melo var agrestis. Plant Breeding, 119, 329-334.
14. Ippolito, A., Schena, L., Nigro, F., Ligorio, V., & Yassen, T. (2004). Real-time detection of Phytophtora nicotianae and P. citrophtora in citrus roots and soils. European Journal of Plant Pathology, 110, 833-843.
15. Lovic, B. R., Martyn, R. D., & Miller, R. D. (1995). Sequence analysis of the ITS regions of rDNA in Monosporascus spp. to evaluate its potential for PCR-mediated detection. Phytopathology, 85, 655-661.
16. Martyn, R. D., & Miller, M. E. (1996). Monosporascus root rot and vine decline. An emerging disease of melons worldwide. Plant Disease, 80, 716-725.
17. McCartney, H. A., Foster, S. J., Fraaije, B. A., & Ward, E. (2003). Molecular diagnostics for fungal plant pathogens. Pest Management Science, 59, 129-142.
18. Mertely, J. C., Martyn, R. D., Miller, M. E., & Bruton, B. D. (1993). An expanded host range for the muskmelon pathogen Monosporascus cannonballus. Plant Disease, 77, 667-673.
19. Stanghellini, M. E., Kim, D. H., Waugh, M. M., Ferrin, D. M., Alcantara, T., & Rasmussen, S. L. (2004). Infection and colonization of melon roots by Monosporascus cannonballus in two cropping seasons in Arizona and California. Plant Pathology, 53, 54-57.
20. van de Graaf, P., Lees, A. K., Cullen, D. W., & Duncan, J. M. (2003). Detection and quantification of Spongospora subterranea in soil, water and plant tissue samples using real-time PCR. European Journal of Plant Pathology, 109, 589-597.